LOCUS       62056_19370        5309 bp DNA     circular SYN 16-MAY-2020
DEFINITION  Cloning vector pSC101-mKate2-AattB-AattP-BFP-RBS8-GP44, complete 
            sequence.
ACCESSION   MN623111
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Guo L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2019) Jiangnan University, State Key Laboratory of
            Food Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122,
            China
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-OCT-2019) Jiangnan University, State Key Laboratory of Food 
            Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, 
            China"
FEATURES             Location/Qualifiers
     source          1..5309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(100..129)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             complement(185..880)
                     /label=mKate2
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     promoter        complement(967..1001)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119)"
     terminator      1018..1089
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             1183..1881
                     /label=TagBFP
                     /note="monomeric blue fluorescent protein"
     gene            1908..2126
                     /gene="gp44"
                     /label=gp44
     CDS             1908..2126
                     /codon_start=1
                     /transl_table=11
                     /gene="gp44"
                     /product="recombination directionality factor"
                     /label=gp44
                     /note="Gp44"
                     /protein_id="QJR97788.1"
                     /translation="MSEVLVSASYEGYESNSINFTEINNIVKERFKKIDEVERKKRAET
                     FNKKYEVTKELVNGHLREIIIPRRLIK"
     terminator      2168..2199
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     terminator      2324..2418
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2910..3132
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             3180..4127
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     CDS             4419..5276
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      5290..5309
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg tttgccacct
       61 gacgtctaag aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc ccacccgtga
      121 aggtgagcca gtgagttgat tgctacgtaa ttagttagtt agcccttagt gactcgaatt
      181 ctcaacgatg tcctaatttc gacggaaggt cacaataacg ggctacggct acttcatgtt
      241 gttcaacgta ggtttcttta tcagcttctt taatacgttc taagcgacga tcaacatagt
      301 atactccagg cattttaagg tttttagcgg gttttttgga acgataggtt gttttcaggt
      361 tgcagattaa atgaccgcct ccaactaatt tcagtgccat gtctgcgcgt ccttctaagc
      421 caccgtccgc aggatataag gtttctgttg atgcttccca accaagagtt tttttttgca
      481 ttacgggtcc gttactcggg aaatttacgc cacggatttt aacattgtaa atcagacagc
      541 catcttgtaa actcgtatct tgagttgctg taagtacacc gccatcttca taagttgtta
      601 cacgttccca ggtaaagcct tcaggaaagg actgtttaaa aaagtcagga atgccttggg
      661 tgtgattgat aaaagtttta gacccgtaca taaagctcgt tgcgagaatg tcaaacgcaa
      721 agggcagggg tccaccttca actgctttga tgcgcattgt ttgtgtgcct tcatatggtt
      781 taccttcacc ttcggatgtg catttgaaat gatgattgtt gacagtacct tccatatata
      841 atttcatgtg catattttct ttaattaatt ctgagagcat gatatctttc tcctctttaa
      901 tgcttaactt ttcggatcaa gctatgaagg acgcaaagag ggaactaaac acttggatcc
      961 cccggggcta gcattatacc taggactgag ctagctgtca atactagagc caggcatcaa
     1021 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1081 aacgctctct actagagagg tcatatgttt agtttctcgt ttcttcttct tccaacgaga
     1141 gaaaacgagg aactaaggta ccttaaagag gagaaagtcg acatgagcga gctgattaag
     1201 gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc
     1261 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc
     1321 gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc
     1381 aagaccttca tcgaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag
     1441 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag
     1501 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
     1561 acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgagacg
     1621 ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc
     1681 gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc cgctaagaac
     1741 ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac
     1801 aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc
     1861 aaactggggc acaagctcaa ttaagatcta aagaggagaa actcgagatg agcgaagttt
     1921 tagtatcagc tagctatgaa ggctatgagt caaatagtat taatttcaca gaaataaaca
     1981 acattgtaaa agagcgattt aaaaagattg atgaagttga gcgcaaaaaa agagctgaaa
     2041 catttaataa aaagtatgaa gtcacaaaag agcttgtaaa tggacattta cgcgaaatta
     2101 ttatcccaag gaggctaata aaataactgc aggagtcact aagggttagt tagttagatt
     2161 agcagaaagt caaaagcctc cgaccggagg cttttgacta aaacttccct tggggttatc
     2221 attggggctc actcaaaggc ggtaatcaga taaaaaaaat ccttagcttt cgctgaggat
     2281 gatttctgct agagctgtca gaccaagttt acgagctcgc ttggactcct gttgatagat
     2341 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg
     2401 ttttttattg gtgagaatcc aagcactagg gacagtaaga cgggtaagcc tgttgatgat
     2461 accgctgcct tactgggtgc attagccagt ctgaatgacc tgtcacgggg taatccgaag
     2521 tggccagact ggaaaatcag agggcaggaa ctgctgaaca gcaaaaagtc agatagcacc
     2581 gcatagcaga cccgccataa aacgccctga gaagcccgtg acgggctttt cttgtattat
     2641 gggtagtttc cttgcatgaa tccataaaag gcgcctgtag tgccatttac ccccattcac
     2701 tgccagagcc gtgagtgcag cgaactgaat gtcacgaaaa agacggcgac tcaggtgcct
     2761 gatggtcgga gacaaaagga atattcagcg atttgcccga gcttgcgagg gtgctactta
     2821 agcctttagg gttttaaggt ctgttttgta gaggagcaaa cagcgtttgc gacatccttt
     2881 tgtaatactg cggaactgac taaagtagtg agttatacac agggctggga tctattcttt
     2941 ttatcttttt ttattctttc tttattctat aaattataac cacttgaata taaacaaaaa
     3001 aaacacacaa aggtctagcg gaatttacag agggtctagc agaatttaca agttttccag
     3061 caaaggtcta gcagaattta cagataccca caactcaaag gaaaaggaca tgtaattatc
     3121 attgactagc ccatctcaat tggtatagtg attaaaatca cctagaccaa ttgagatgta
     3181 tgtctgaatt agttgttttc aaagcaaatg aactagcgat tagtcgctat gacttaacgg
     3241 agcatgaaac caagctaatt ttatgctgtg tggcactact caaccccacg attgaaaacc
     3301 ctacaaggaa agaacggacg gtatcgttca cttataacca atacgctcag atgatgaaca
     3361 tcagtaggga aaatgcttat ggtgtattag ctaaagcaac cagagagctg atgacgagaa
     3421 ctgtggaaat caggaatcct ttggttaaag gctttgagat tttccagtgg acaaactgtg
     3481 ccaagttctc aagcgaaaaa ttagaattag tttttagtga agggatattg ccttatcttt
     3541 tccagttaaa aaaattcata aaatataatc tggaacatgt taagtctttt gaaaacaaat
     3601 actctatgag gatttatgag tggttattaa aagaactaac acaaaagaaa actcacaagg
     3661 caaatataga gattagcctt gatgaattta agttcatgtt aatgcttgaa aataactacc
     3721 atgagtttaa aaggcttaac caatgggttt tgaaaccaat aagtaaagat ttgaacactt
     3781 acagcaatat gaaattggtg gttgataagc gaggccgccc gactgatacg ttgattttcc
     3841 aagttgaact agatagacaa atggatctcg taaccgaact tgagaacaac cagataaaaa
     3901 tgaatggtga caaaatacca acaaccatta catcagattc ctacctacat aacggactaa
     3961 gaaaaacact acacgatgct ttaactgcaa aaattcagtt caccagtttt gaggcaaaat
     4021 ttttgggtga catgcaaagt aagtatgatc tcaatggttc gttctcatgg ctcacgcaaa
     4081 aacaacgaac cacactagag aacatactgg ctaaatacgg aaggatctga ggttcttatg
     4141 gctcttgtat ctatcagtga agcatcaaga ctaacaaaca aaagtagaac aactgttcac
     4201 cgttacatat caaagggaaa actgtccata tgcacagatg aaaacggtgt aaaaaagata
     4261 gatacatcag agcttttacg agtttttggt gcattcaaag ctgttcacca tgaacagatc
     4321 gacaatgtaa ctactagagc taaatacatt caaatatcta tccgctcatg agacaataac
     4381 cctgataaat gcttcaataa tattgaaaaa ggaagaatat gagtattcaa catttccgtg
     4441 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4501 tggtgaaagt aaaagatgcc gaagatcagt tgggcgcacg tgtgggttac atcgaactgg
     4561 acctcaacag cggtaagatt cttgagagtt ttcgccccga agaacgtttc ccaatgatga
     4621 gcacttttaa agttctgctc tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4681 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4741 aaaagcatct tacggacggc atgacagtac gcgaattatg cagcgctgcc ataaccacga
     4801 gtgataacac ggcggccaac ttacttctga caacgatcgg gggaccgaag gagcttaccg
     4861 cttttttgca caacatgggt gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4921 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagctatg gcaacaacgt
     4981 tgcgcaaact cttaactggc gaacttctta ctctcgcttc ccggcaacaa ttaatagact
     5041 ggatggaggc ggataaagtt gcgggaccac ttctgcgctc ggcccttccg gctggctggt
     5101 ttattgctga taaatctgga gccggtgagc gtgggtcccg cggtattatt gcagccctgg
     5161 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagc caggcaacta
     5221 tggacgaacg taatcgccag atcgctgaga taggtgcctc cctgattaag cattggtaat
     5281 aatactagct ccggcaaaaa aacgggcaa
//
