LOCUS       62056_19365        5348 bp DNA     circular SYN 16-MAY-2020
DEFINITION  Cloning vector pSC101-mKate2-AattB-AattP-BFP-RBS8-GP44-AAV, complete
            sequence.
ACCESSION   MN623113
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5348)
  AUTHORS   Guo L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2019) Jiangnan University, State Key Laboratory of
            Food Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122,
            China
REFERENCE   2  (bases 1 to 5348)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-OCT-2019) Jiangnan University, State Key Laboratory of Food 
            Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, 
            China"
FEATURES             Location/Qualifiers
     source          1..5348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(100..129)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             complement(185..880)
                     /label=mKate2
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
     promoter        complement(967..1001)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119)"
     terminator      1018..1089
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             1183..1881
                     /label=TagBFP
                     /note="monomeric blue fluorescent protein"
     gene            1908..2123
                     /gene="gp44"
                     /label=gp44
     CDS             1908..2123
                     /codon_start=1
                     /transl_table=11
                     /gene="gp44"
                     /product="recombination directionality factor"
                     /label=gp44
                     /note="Gp44"
                     /protein_id="QJR97795.1"
                     /translation="MSEVLVSASYEGYESNSINFTEINNIVKERFKKIDEVERKKRAET
                     FNKKYEVTKELVNGHLREIIIPRRLIK"
     terminator      2207..2238
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     terminator      2363..2457
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2949..3171
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             3219..4166
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     CDS             4458..5315
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      5329..5348
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg tttgccacct
       61 gacgtctaag aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc ccacccgtga
      121 aggtgagcca gtgagttgat tgctacgtaa ttagttagtt agcccttagt gactcgaatt
      181 ctcaacgatg tcctaatttc gacggaaggt cacaataacg ggctacggct acttcatgtt
      241 gttcaacgta ggtttcttta tcagcttctt taatacgttc taagcgacga tcaacatagt
      301 atactccagg cattttaagg tttttagcgg gttttttgga acgataggtt gttttcaggt
      361 tgcagattaa atgaccgcct ccaactaatt tcagtgccat gtctgcgcgt ccttctaagc
      421 caccgtccgc aggatataag gtttctgttg atgcttccca accaagagtt tttttttgca
      481 ttacgggtcc gttactcggg aaatttacgc cacggatttt aacattgtaa atcagacagc
      541 catcttgtaa actcgtatct tgagttgctg taagtacacc gccatcttca taagttgtta
      601 cacgttccca ggtaaagcct tcaggaaagg actgtttaaa aaagtcagga atgccttggg
      661 tgtgattgat aaaagtttta gacccgtaca taaagctcgt tgcgagaatg tcaaacgcaa
      721 agggcagggg tccaccttca actgctttga tgcgcattgt ttgtgtgcct tcatatggtt
      781 taccttcacc ttcggatgtg catttgaaat gatgattgtt gacagtacct tccatatata
      841 atttcatgtg catattttct ttaattaatt ctgagagcat gatatctttc tcctctttaa
      901 tgcttaactt ttcggatcaa gctatgaagg acgcaaagag ggaactaaac acttggatcc
      961 cccggggcta gcattatacc taggactgag ctagctgtca atactagagc caggcatcaa
     1021 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1081 aacgctctct actagagagg tcatatgttt agtttctcgt ttcttcttct tccaacgaga
     1141 gaaaacgagg aactaaggta ccttaaagag gagaaagtcg acatgagcga gctgattaag
     1201 gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc
     1261 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc
     1321 gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc
     1381 aagaccttca tcgaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag
     1441 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag
     1501 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
     1561 acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgagacg
     1621 ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc
     1681 gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc cgctaagaac
     1741 ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac
     1801 aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc
     1861 aaactggggc acaagctcaa ttaagatcta aagaggagaa actcgagatg agcgaagttt
     1921 tagtatcagc tagctatgaa ggctatgagt caaatagtat taatttcaca gaaataaaca
     1981 acattgtaaa agagcgattt aaaaagattg atgaagttga gcgcaaaaaa agagctgaaa
     2041 catttaataa aaagtatgaa gtcacaaaag agcttgtaaa tggacattta cgcgaaatta
     2101 ttatcccaag gaggctaata aaatctagag cagcaaacga cgaaaactac gctgctgctg
     2161 tttaactgca ggagtcacta agggttagtt agttagatta gcagaaagtc aaaagcctcc
     2221 gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca ctcaaaggcg
     2281 gtaatcagat aaaaaaaatc cttagctttc gctgaggatg atttctgcta gagctgtcag
     2341 accaagttta cgagctcgct tggactcctg ttgatagatc cagtaatgac ctcagaactc
     2401 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca
     2461 agcactaggg acagtaagac gggtaagcct gttgatgata ccgctgcctt actgggtgca
     2521 ttagccagtc tgaatgacct gtcacggggt aatccgaagt ggccagactg gaaaatcaga
     2581 gggcaggaac tgctgaacag caaaaagtca gatagcaccg catagcagac ccgccataaa
     2641 acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat
     2701 ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg tgagtgcagc
     2761 gaactgaatg tcacgaaaaa gacggcgact caggtgcctg atggtcggag acaaaaggaa
     2821 tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc
     2881 tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc ggaactgact
     2941 aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt tattctttct
     3001 ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg
     3061 aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag cagaatttac
     3121 agatacccac aactcaaagg aaaaggacat gtaattatca ttgactagcc catctcaatt
     3181 ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta gttgttttca
     3241 aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc aagctaattt
     3301 tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg
     3361 tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa aatgcttatg
     3421 gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc aggaatcctt
     3481 tggttaaagg ctttgagatt ttccagtgga caaactgtgc caagttctca agcgaaaaat
     3541 tagaattagt ttttagtgaa gggatattgc cttatctttt ccagttaaaa aaattcataa
     3601 aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg atttatgagt
     3661 ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag attagccttg
     3721 atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa aggcttaacc
     3781 aatgggtttt gaaaccaata agtaaagatt tgaacactta cagcaatatg aaattggtgg
     3841 ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta gatagacaaa
     3901 tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac aaaataccaa
     3961 caaccattac atcagattcc tacctacata acggactaag aaaaacacta cacgatgctt
     4021 taactgcaaa aattcagttc accagttttg aggcaaaatt tttgggtgac atgcaaagta
     4081 agtatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc acactagaga
     4141 acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc tatcagtgaa
     4201 gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc aaagggaaaa
     4261 ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga gcttttacga
     4321 gtttttggtg cattcaaagc tgttcaccat gaacagatcg acaatgtaac tactagagct
     4381 aaatacattc aaatatctat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4441 attgaaaaag gaagaatatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4501 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgccg
     4561 aagatcagtt gggcgcacgt gtgggttaca tcgaactgga cctcaacagc ggtaagattc
     4621 ttgagagttt tcgccccgaa gaacgtttcc caatgatgag cacttttaaa gttctgctct
     4681 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     4741 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggacggca
     4801 tgacagtacg cgaattatgc agcgctgcca taaccacgag tgataacacg gcggccaact
     4861 tacttctgac aacgatcggg ggaccgaagg agcttaccgc ttttttgcac aacatgggtg
     4921 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4981 agcgtgacac cacgatgcct gtagctatgg caacaacgtt gcgcaaactc ttaactggcg
     5041 aacttcttac tctcgcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5101 cgggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5161 ccggtgagcg tgggtcccgc ggtattattg cagccctggg gccagatggt aagccctccc
     5221 gtatcgtagt tatctacacg acggggagcc aggcaactat ggacgaacgt aatcgccaga
     5281 tcgctgagat aggtgcctcc ctgattaagc attggtaata atactagctc cggcaaaaaa
     5341 acgggcaa
//
