LOCUS       V016318                 7392 bp    DNA     circular SYN 16-MAY-2020
DEFINITION  Exported.
ACCESSION   V016318
VERSION     V016318
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Guo L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2019) Jiangnan University, State Key Laboratory of
            Food Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122,
            China
REFERENCE   2  (bases 1 to 7392)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (28-OCT-2019) Jiangnan University, State Key Laboratory of Food
            Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122,
            China"
FEATURES             Location/Qualifiers
     source          1..7392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(100..129)
                     /label="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     CDS             complement(185..367)
                     /gene="csrA"
                     /label="Carbon storage regulator"
                     /note="Carbon storage regulator from Escherichia coli
                     (strain K12). Accession#: P69913"
     promoter        complement(450..484)
                     /label="J23119 promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119)"
     terminator      501..572
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     gene            667..2346
                     /gene="phaC"
                     /label="phaC"
     CDS             667..2346
                     /codon_start=1
                     /transl_table=11
                     /gene="phaC"
                     /product="polyhydroxyalkanoate synthase"
                     /label="phaC"
                     /protein_id="QJR97798.1"
                     /translation="MSNKNSDDLNRQASENTLGLNPVIGLRGKDLLTSARMVLTQAIKQ
                     PIHSVKHVAHFGIELKNVMFGKSKLQPESDDRRFNDPAWSQNPLYKRYLQTYLAWRKEL
                     HDWIGNSKLSEQDINRAHFVITLMTEAMAPTNSAANPAAVKRFFETGGKSLLDGLTHLA
                     KDLVNNGGMPSQVDMGAFEVGKSLGTTEGAVVFRNDVLELIQYRPTTEQVHERPLLVVP
                     PQINKFYVFDLSPDKSLARFCLSNNQQTFIVSWRNPTKAQREWGLSTYIDALKEAVDVV
                     SAITGSKDINMLGACSGGITCTALLGHYAALGEKKVNALTLLVTVLDTTLDSQVALFVD
                     EKTLEAAKRHSYQAGVLEGRDMAKVFAWMRPNDLIWNYWVNNYLLGNEPPVFDILFWNN
                     DTTRLPAAFHGDLIEMFKNNPLVRANALEVSGTPIDLKQVTADIYSLAGTNDHITPWKS
                     CYKSAQLFGGKVEFVLSSSGHIKSILNPPGNPKSRYMTSTDMPATANEWQENSTKHTDS
                     WWLHWQAWQAERSGKLKKSPTSLGNKAYPSGEAAPGTYVHER"
     gene            2374..3927
                     /gene="pct"
                     /label="pct"
     CDS             2374..3927
                     /codon_start=1
                     /transl_table=11
                     /gene="pct"
                     /product="propionyl-CoA transferase"
                     /label="pct"
                     /note="PCT"
                     /protein_id="QJR97799.1"
                     /translation="MRKVEIITAEQAAQLVKDNDTITSIGFVSSAHPEALTKALEKRFL
                     DTNTPQNLTYIYAGSQDKRDGRAAEHLAHTGLLKRAIIGHWQTVPAIGKLAVENKIEAY
                     NFSQGTLVHWFRALAGHKLGVFTDIGLETFLDPRQLGGKLNDVTKEDLVKLIEVDGHEQ
                     LFYPTFPVNVAFLRGTYADESGNITMDEEIGPFESTSVAQAVHNCGGKVVVQVKDVVAH
                     GSLDPRMVKIPGIYVDYVVVAAPEDHQQTYDCEYDPSLSGEHRAPEGATDAALPMSAKK
                     IIGRRGALELTENAVVNLGVGAPEYVASVAGEEGIADTITLTVEGGAIGGVPQGGARFG
                     SSRNADAIIDHTYQFDFYDGGGLDIAYLGLAQCDGSGNINVSKFGTNVAGCGGFPNISQ
                     QTPNVYFCGTFTAGGLKIAVEDGKVKILQEGKAKKFIKAVDQITFNGSYAARNGKHVLY
                     ITERCVFELTKEGLKLIEVAPGIDIEKDILAHMDFKPIIDNPKLMDARLFQDGPMGLKK
                     "
     gene            3952..4167
                     /gene="gp44"
                     /label="gp44"
     CDS             3952..4167
                     /codon_start=1
                     /transl_table=11
                     /gene="gp44"
                     /product="recombination directionality factor"
                     /label="gp44"
                     /note="Gp44"
                     /protein_id="QJR97800.1"
                     /translation="MSEVLVSASYEGYESNSINFTEINNIVKERFKKIDEVERKKRAET
                     FNKKYEVTKELVNGHLREIIIPRRLIK"
     terminator      4251..4282
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     terminator      4407..4501
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     rep_origin      4993..5215
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             5263..6210
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     CDS             6502..7359
                     /label="AmpR"
                     /note="beta-lactamase"
     terminator      7373..7392
                     /label="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg tttgccacct
       61 gacgtctaag aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc ccacccgtga
      121 aggtgagcca gtgagttgat tgctacgtaa ttagttagtt agcccttagt gactcgaatt
      181 cttagtaact ggactgctgg gatttttcag cctggatacg ctggtagatc tcttcacggt
      241 gaacagaaac ttccttcggg gcatttacgc caatacgtac ctggttgccc tttaccccta
      301 aaactgtcac ggtgacctca tccccaatca tgagggtctc accaactcga cgagtcagaa
      361 tcagcatgat atcgtcctgt gtgagcttaa cttttcggat caagctatga aggacgcaaa
      421 gagggaacta aacacttgga tcccccgggg ctagcattat acctaggact gagctagctg
      481 tcaatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      541 gttttatctg ttgtttgtcg gtgaacgctc tctactagag aggtcatatg tttagtttct
      601 cgtttcttct tcttccaacg agagaaaacg aggaactaag gtaccattaa agaggagaaa
      661 acgcgtatga gcaacaaaaa cagcgacgac ctgaaccgtc aggcttccga gaacacgctg
      721 ggtctgaacc ctgtaatcgg tctgcgtggc aaggatctgc tgacttctgc acgtatggtc
      781 ctgactcaag caatcaagca gccaatccac tctgtcaagc acgtcgcaca cttcggtatc
      841 gagctgaaga acgtcatgtt cggtaaaagc aaactgcaac cggagtccga tgaccgtcgt
      901 ttcaacgacc cggcatggtc tcagaaccct ctgtacaaac gttacctgca aacctacctg
      961 gcctggcgta aagagctgca cgactggatc ggtaactcca aactgtccga gcaggacatc
     1021 aaccgtgcac acttcgtcat cacgctgatg accgaagcta tggcacctac taactccgct
     1081 gctaacccag cagcagtgaa acgcttcttc gaaaccggtg gcaaaagcct gctggacggt
     1141 ctgactcatc tggcaaagga cctggttaac aacggtggta tgccaagcca ggttgacatg
     1201 ggtgcattcg aagtgggcaa atctctgggt actaccgaag gtgcagtggt gttccgtaac
     1261 gacgtgctgg aactgatcca gtaccgtcca actactgaac aggtgcacga acgtccactg
     1321 ctggtagtgc cgccgcaaat taacaaattc tacgtgttcg acctgtcccc ggacaaatcc
     1381 ctggctcgtt tctgtctgtc taacaaccag cagaccttca tcgtgtcctg gcgtaacccg
     1441 actaaagcgc agcgtgaatg gggtctgtct acttacatcg acgctctgaa agaagccgtt
     1501 gacgttgttt ccgccatcac gggtagcaaa gacatcaaca tgctgggcgc ttgctccggt
     1561 ggtattacct gcactgctct gctgggtcat tacgctgctc tgggtgagaa gaaagtgaac
     1621 gctctgactc tgctggttac ggttctggat accaccctgg actctcaggt tgctctgttc
     1681 gttgatgaga aaacgctgga agccgctaaa cgccactctt atcaggcggg tgtactggaa
     1741 ggccgtgata tggcgaaagt tttcgcgtgg atgcgcccga atgatctgat ctggaactac
     1801 tgggttaaca actacctgct gggcaacgaa ccgccggttt ttgatatcct gttttggaac
     1861 aacgatacca cccgcctgcc ggcggcgttt cacggcgatc tgattgaaat gtttaaaaac
     1921 aacccgctgg tacgcgcgaa cgcgctggaa gtaagcggca ccccgattga tctgaaacag
     1981 gttaccgcgg atatctatag cctggcgggc accaatgatc acattacccc gtggaaatcc
     2041 tgttacaaaa gcgcccagct gtttggcggc aaagtagaat ttgtactgag ctccagcggc
     2101 cacattaaga gcattctgaa tccgccgggc aatccgaaat cccgctatat gacctctacc
     2161 gatatgccgg cgaccgcgaa tgaatggcag gaaaattcta ccaaacatac cgattcttgg
     2221 tggctgcatt ggcaggcgtg gcaggccgaa cgctctggca aactgaagaa atctccgacc
     2281 tctctgggca acaaagccta tccgtctggc gaagcggcgc cgggcaccta tgttcatgaa
     2341 cgctaaaagc tttcacacag gaaagggatc ctcatgagaa aagtagaaat cattactgct
     2401 gaacaagcag ctcagctcgt aaaagacaac gacacgatta cgtctatcgg ctttgtcagc
     2461 agcgcccatc cggaagcact gaccaaagct ttggaaaaac ggttcctgga cacgaacacc
     2521 ccgcagaact tgacctacat ctatgcaggc tctcaggaca aacgcgatgg ccgtgccgct
     2581 gaacatctgg cacacacagg ccttttgaaa cgcgccatca tcggtcactg gcagactgta
     2641 ccggctatcg gtaaactggc tgtcgaaaac aagattgaag cttacaactt ctcgcagggc
     2701 acgttggtcc actggttccg cgccttggca ggtcataagc tcggcgtctt caccgacatc
     2761 ggtctggaaa ctttcctcga tccccgtcag ctcggcggca agctcaatga cgtaaccaaa
     2821 gaagacctcg tcaaactgat cgaagtcgat ggtcatgaac agcttttcta cccgaccttc
     2881 ccggtcaacg tagctttcct ccgcggtacg tatgctgatg aatccggcaa tatcaccatg
     2941 gacgaagaaa tcgggccttt cgaaagcact tccgtagccc aggccgttca caactgtggc
     3001 ggtaaagtcg tcgtccaggt caaagacgtc gtcgctcacg gcagcctcga cccgcgcatg
     3061 gtcaagatcc ctggcatcta tgtcgactac gtcgtcgtag cagctccgga agaccatcag
     3121 cagacgtatg actgcgaata cgatccgtcc ctcagcggtg aacatcgtgc tcctgaaggc
     3181 gctaccgatg cagctctccc catgagcgct aagaaaatca tcggccgccg cggcgctttg
     3241 gaattgactg aaaacgctgt cgtcaacctc ggcgtcggtg ctccggaata cgttgcttct
     3301 gttgccggtg aagaaggtat cgccgatacc attaccctga ccgtcgaagg tggcgccatc
     3361 ggtggcgtac cgcagggcgg tgcccgcttc ggttcgtccc gcaatgccga tgccatcatc
     3421 gaccacacct atcagttcga cttctacgat ggcggcggtc tggacatcgc ttacctcggc
     3481 ctggcccagt gcgatggctc gggcaacatc aacgtcagca agttcggtac taacgttgcc
     3541 ggctgcggcg gtttccccaa catttcccag cagacaccga atgtttactt ctgcggcacc
     3601 ttcacggctg gcggcttgaa aatcgctgtc gaagacggca aagtcaagat cctccaggaa
     3661 ggcaaagcca agaagttcat caaagctgtc gaccagatca ctttcaacgg ttcctatgca
     3721 gcccgcaacg gcaaacacgt tctctacatc acagaacgct gcgtatttga actgaccaaa
     3781 gaaggcttga aactcatcga agtcgcaccg ggcatcgata ttgaaaaaga tatcctcgct
     3841 cacatggact tcaagccgat cattgataat ccgaaactca tggatgcccg cctcttccag
     3901 gacggtccca tgggactgaa aaaataaaga tctaaagagg agaaactcga gatgagcgaa
     3961 gttttagtat cagctagcta tgaaggctat gagtcaaata gtattaattt cacagaaata
     4021 aacaacattg taaaagagcg atttaaaaag attgatgaag ttgagcgcaa aaaaagagct
     4081 gaaacattta ataaaaagta tgaagtcaca aaagagcttg taaatggaca tttacgcgaa
     4141 attattatcc caaggaggct aataaaatct agagcagcaa acgacgaaaa ctacgctgct
     4201 gctgtttaac tgcaggagtc actaagggtt agttagttag attagcagaa agtcaaaagc
     4261 ctccgaccgg aggcttttga ctaaaacttc ccttggggtt atcattgggg ctcactcaaa
     4321 ggcggtaatc agataaaaaa aatccttagc tttcgctgag gatgatttct gctagagctg
     4381 tcagaccaag tttacgagct cgcttggact cctgttgata gatccagtaa tgacctcaga
     4441 actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa
     4501 tccaagcact agggacagta agacgggtaa gcctgttgat gataccgctg ccttactggg
     4561 tgcattagcc agtctgaatg acctgtcacg gggtaatccg aagtggccag actggaaaat
     4621 cagagggcag gaactgctga acagcaaaaa gtcagatagc accgcatagc agacccgcca
     4681 taaaacgccc tgagaagccc gtgacgggct tttcttgtat tatgggtagt ttccttgcat
     4741 gaatccataa aaggcgcctg tagtgccatt tacccccatt cactgccaga gccgtgagtg
     4801 cagcgaactg aatgtcacga aaaagacggc gactcaggtg cctgatggtc ggagacaaaa
     4861 ggaatattca gcgatttgcc cgagcttgcg agggtgctac ttaagccttt agggttttaa
     4921 ggtctgtttt gtagaggagc aaacagcgtt tgcgacatcc ttttgtaata ctgcggaact
     4981 gactaaagta gtgagttata cacagggctg ggatctattc tttttatctt tttttattct
     5041 ttctttattc tataaattat aaccacttga atataaacaa aaaaaacaca caaaggtcta
     5101 gcggaattta cagagggtct agcagaattt acaagttttc cagcaaaggt ctagcagaat
     5161 ttacagatac ccacaactca aaggaaaagg acatgtaatt atcattgact agcccatctc
     5221 aattggtata gtgattaaaa tcacctagac caattgagat gtatgtctga attagttgtt
     5281 ttcaaagcaa atgaactagc gattagtcgc tatgacttaa cggagcatga aaccaagcta
     5341 attttatgct gtgtggcact actcaacccc acgattgaaa accctacaag gaaagaacgg
     5401 acggtatcgt tcacttataa ccaatacgct cagatgatga acatcagtag ggaaaatgct
     5461 tatggtgtat tagctaaagc aaccagagag ctgatgacga gaactgtgga aatcaggaat
     5521 cctttggtta aaggctttga gattttccag tggacaaact gtgccaagtt ctcaagcgaa
     5581 aaattagaat tagtttttag tgaagggata ttgccttatc ttttccagtt aaaaaaattc
     5641 ataaaatata atctggaaca tgttaagtct tttgaaaaca aatactctat gaggatttat
     5701 gagtggttat taaaagaact aacacaaaag aaaactcaca aggcaaatat agagattagc
     5761 cttgatgaat ttaagttcat gttaatgctt gaaaataact accatgagtt taaaaggctt
     5821 aaccaatggg ttttgaaacc aataagtaaa gatttgaaca cttacagcaa tatgaaattg
     5881 gtggttgata agcgaggccg cccgactgat acgttgattt tccaagttga actagataga
     5941 caaatggatc tcgtaaccga acttgagaac aaccagataa aaatgaatgg tgacaaaata
     6001 ccaacaacca ttacatcaga ttcctaccta cataacggac taagaaaaac actacacgat
     6061 gctttaactg caaaaattca gttcaccagt tttgaggcaa aatttttggg tgacatgcaa
     6121 agtaagtatg atctcaatgg ttcgttctca tggctcacgc aaaaacaacg aaccacacta
     6181 gagaacatac tggctaaata cggaaggatc tgaggttctt atggctcttg tatctatcag
     6241 tgaagcatca agactaacaa acaaaagtag aacaactgtt caccgttaca tatcaaaggg
     6301 aaaactgtcc atatgcacag atgaaaacgg tgtaaaaaag atagatacat cagagctttt
     6361 acgagttttt ggtgcattca aagctgttca ccatgaacag atcgacaatg taactactag
     6421 agctaaatac attcaaatat ctatccgctc atgagacaat aaccctgata aatgcttcaa
     6481 taatattgaa aaaggaagaa tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6541 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6601 gccgaagatc agttgggcgc acgtgtgggt tacatcgaac tggacctcaa cagcggtaag
     6661 attcttgaga gttttcgccc cgaagaacgt ttcccaatga tgagcacttt taaagttctg
     6721 ctctgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     6781 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggac
     6841 ggcatgacag tacgcgaatt atgcagcgct gccataacca cgagtgataa cacggcggcc
     6901 aacttacttc tgacaacgat cgggggaccg aaggagctta ccgctttttt gcacaacatg
     6961 ggtgatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7021 gacgagcgtg acaccacgat gcctgtagct atggcaacaa cgttgcgcaa actcttaact
     7081 ggcgaacttc ttactctcgc ttcccggcaa caattaatag actggatgga ggcggataaa
     7141 gttgcgggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7201 ggagccggtg agcgtgggtc ccgcggtatt attgcagccc tggggccaga tggtaagccc
     7261 tcccgtatcg tagttatcta cacgacgggg agccaggcaa ctatggacga acgtaatcgc
     7321 cagatcgctg agataggtgc ctccctgatt aagcattggt aataatacta gctccggcaa
     7381 aaaaacgggc aa
//
