LOCUS       62056_19350        7189 bp DNA     circular SYN 16-MAY-2020
DEFINITION  Cloning vector pSC101-Bio-phaC-pct-GP44-AAV, complete sequence.
ACCESSION   MN623115
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7189)
  AUTHORS   Guo L.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2019) Jiangnan University, State Key Laboratory of
            Food Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122,
            China
REFERENCE   2  (bases 1 to 7189)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (28-OCT-2019) Jiangnan University, State Key Laboratory of Food 
            Science and Technology, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, 
            China"
FEATURES             Location/Qualifiers
     source          1..7189
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(100..129)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     promoter        complement(247..281)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119)"
     terminator      298..369
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     gene            464..2143
                     /gene="phaC"
                     /label=phaC
     CDS             464..2143
                     /codon_start=1
                     /transl_table=11
                     /gene="phaC"
                     /product="polyhydroxyalkanoate synthase"
                     /label=phaC
                     /protein_id="QJR97802.1"
                     /translation="MSNKNSDDLNRQASENTLGLNPVIGLRGKDLLTSARMVLTQAIKQ
                     PIHSVKHVAHFGIELKNVMFGKSKLQPESDDRRFNDPAWSQNPLYKRYLQTYLAWRKEL
                     HDWIGNSKLSEQDINRAHFVITLMTEAMAPTNSAANPAAVKRFFETGGKSLLDGLTHLA
                     KDLVNNGGMPSQVDMGAFEVGKSLGTTEGAVVFRNDVLELIQYRPTTEQVHERPLLVVP
                     PQINKFYVFDLSPDKSLARFCLSNNQQTFIVSWRNPTKAQREWGLSTYIDALKEAVDVV
                     SAITGSKDINMLGACSGGITCTALLGHYAALGEKKVNALTLLVTVLDTTLDSQVALFVD
                     EKTLEAAKRHSYQAGVLEGRDMAKVFAWMRPNDLIWNYWVNNYLLGNEPPVFDILFWNN
                     DTTRLPAAFHGDLIEMFKNNPLVRANALEVSGTPIDLKQVTADIYSLAGTNDHITPWKS
                     CYKSAQLFGGKVEFVLSSSGHIKSILNPPGNPKSRYMTSTDMPATANEWQENSTKHTDS
                     WWLHWQAWQAERSGKLKKSPTSLGNKAYPSGEAAPGTYVHER"
     gene            2171..3724
                     /gene="pct"
                     /label=pct
     CDS             2171..3724
                     /codon_start=1
                     /transl_table=11
                     /gene="pct"
                     /product="propionyl-CoA transferase"
                     /label=pct
                     /note="PCT"
                     /protein_id="QJR97803.1"
                     /translation="MRKVEIITAEQAAQLVKDNDTITSIGFVSSAHPEALTKALEKRFL
                     DTNTPQNLTYIYAGSQDKRDGRAAEHLAHTGLLKRAIIGHWQTVPAIGKLAVENKIEAY
                     NFSQGTLVHWFRALAGHKLGVFTDIGLETFLDPRQLGGKLNDVTKEDLVKLIEVDGHEQ
                     LFYPTFPVNVAFLRGTYADESGNITMDEEIGPFESTSVAQAVHNCGGKVVVQVKDVVAH
                     GSLDPRMVKIPGIYVDYVVVAAPEDHQQTYDCEYDPSLSGEHRAPEGATDAALPMSAKK
                     IIGRRGALELTENAVVNLGVGAPEYVASVAGEEGIADTITLTVEGGAIGGVPQGGARFG
                     SSRNADAIIDHTYQFDFYDGGGLDIAYLGLAQCDGSGNINVSKFGTNVAGCGGFPNISQ
                     QTPNVYFCGTFTAGGLKIAVEDGKVKILQEGKAKKFIKAVDQITFNGSYAARNGKHVLY
                     ITERCVFELTKEGLKLIEVAPGIDIEKDILAHMDFKPIIDNPKLMDARLFQDGPMGLKK
                     "
     gene            3749..3964
                     /gene="gp44"
                     /label=gp44
     CDS             3749..3964
                     /codon_start=1
                     /transl_table=11
                     /gene="gp44"
                     /product="recombination directionality factor"
                     /label=gp44
                     /note="Gp44"
                     /protein_id="QJR97804.1"
                     /translation="MSEVLVSASYEGYESNSINFTEINNIVKERFKKIDEVERKKRAET
                     FNKKYEVTKELVNGHLREIIIPRRLIK"
     terminator      4048..4079
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     terminator      4204..4298
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      4790..5012
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             5060..6007
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     CDS             6299..7156
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      7170..7189
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
ORIGIN
        1 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg tttgccacct
       61 gacgtctaag aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc ccacccgtga
      121 aggtgagcca gtgagttgat tgctacgtaa ttagttagtt agcccttagt gactcgaatt
      181 cgcttaactt ttcggatcaa gctatgaagg acgcaaagag ggaactaaac acttggatcc
      241 cccggggcta gcattatacc taggactgag ctagctgtca atactagagc caggcatcaa
      301 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      361 aacgctctct actagagagg tcatatgttt agtttctcgt ttcttcttct tccaacgaga
      421 gaaaacgagg aactaaggta ccattaaaga ggagaaaacg cgtatgagca acaaaaacag
      481 cgacgacctg aaccgtcagg cttccgagaa cacgctgggt ctgaaccctg taatcggtct
      541 gcgtggcaag gatctgctga cttctgcacg tatggtcctg actcaagcaa tcaagcagcc
      601 aatccactct gtcaagcacg tcgcacactt cggtatcgag ctgaagaacg tcatgttcgg
      661 taaaagcaaa ctgcaaccgg agtccgatga ccgtcgtttc aacgacccgg catggtctca
      721 gaaccctctg tacaaacgtt acctgcaaac ctacctggcc tggcgtaaag agctgcacga
      781 ctggatcggt aactccaaac tgtccgagca ggacatcaac cgtgcacact tcgtcatcac
      841 gctgatgacc gaagctatgg cacctactaa ctccgctgct aacccagcag cagtgaaacg
      901 cttcttcgaa accggtggca aaagcctgct ggacggtctg actcatctgg caaaggacct
      961 ggttaacaac ggtggtatgc caagccaggt tgacatgggt gcattcgaag tgggcaaatc
     1021 tctgggtact accgaaggtg cagtggtgtt ccgtaacgac gtgctggaac tgatccagta
     1081 ccgtccaact actgaacagg tgcacgaacg tccactgctg gtagtgccgc cgcaaattaa
     1141 caaattctac gtgttcgacc tgtccccgga caaatccctg gctcgtttct gtctgtctaa
     1201 caaccagcag accttcatcg tgtcctggcg taacccgact aaagcgcagc gtgaatgggg
     1261 tctgtctact tacatcgacg ctctgaaaga agccgttgac gttgtttccg ccatcacggg
     1321 tagcaaagac atcaacatgc tgggcgcttg ctccggtggt attacctgca ctgctctgct
     1381 gggtcattac gctgctctgg gtgagaagaa agtgaacgct ctgactctgc tggttacggt
     1441 tctggatacc accctggact ctcaggttgc tctgttcgtt gatgagaaaa cgctggaagc
     1501 cgctaaacgc cactcttatc aggcgggtgt actggaaggc cgtgatatgg cgaaagtttt
     1561 cgcgtggatg cgcccgaatg atctgatctg gaactactgg gttaacaact acctgctggg
     1621 caacgaaccg ccggtttttg atatcctgtt ttggaacaac gataccaccc gcctgccggc
     1681 ggcgtttcac ggcgatctga ttgaaatgtt taaaaacaac ccgctggtac gcgcgaacgc
     1741 gctggaagta agcggcaccc cgattgatct gaaacaggtt accgcggata tctatagcct
     1801 ggcgggcacc aatgatcaca ttaccccgtg gaaatcctgt tacaaaagcg cccagctgtt
     1861 tggcggcaaa gtagaatttg tactgagctc cagcggccac attaagagca ttctgaatcc
     1921 gccgggcaat ccgaaatccc gctatatgac ctctaccgat atgccggcga ccgcgaatga
     1981 atggcaggaa aattctacca aacataccga ttcttggtgg ctgcattggc aggcgtggca
     2041 ggccgaacgc tctggcaaac tgaagaaatc tccgacctct ctgggcaaca aagcctatcc
     2101 gtctggcgaa gcggcgccgg gcacctatgt tcatgaacgc taaaagcttt cacacaggaa
     2161 agggatcctc atgagaaaag tagaaatcat tactgctgaa caagcagctc agctcgtaaa
     2221 agacaacgac acgattacgt ctatcggctt tgtcagcagc gcccatccgg aagcactgac
     2281 caaagctttg gaaaaacggt tcctggacac gaacaccccg cagaacttga cctacatcta
     2341 tgcaggctct caggacaaac gcgatggccg tgccgctgaa catctggcac acacaggcct
     2401 tttgaaacgc gccatcatcg gtcactggca gactgtaccg gctatcggta aactggctgt
     2461 cgaaaacaag attgaagctt acaacttctc gcagggcacg ttggtccact ggttccgcgc
     2521 cttggcaggt cataagctcg gcgtcttcac cgacatcggt ctggaaactt tcctcgatcc
     2581 ccgtcagctc ggcggcaagc tcaatgacgt aaccaaagaa gacctcgtca aactgatcga
     2641 agtcgatggt catgaacagc ttttctaccc gaccttcccg gtcaacgtag ctttcctccg
     2701 cggtacgtat gctgatgaat ccggcaatat caccatggac gaagaaatcg ggcctttcga
     2761 aagcacttcc gtagcccagg ccgttcacaa ctgtggcggt aaagtcgtcg tccaggtcaa
     2821 agacgtcgtc gctcacggca gcctcgaccc gcgcatggtc aagatccctg gcatctatgt
     2881 cgactacgtc gtcgtagcag ctccggaaga ccatcagcag acgtatgact gcgaatacga
     2941 tccgtccctc agcggtgaac atcgtgctcc tgaaggcgct accgatgcag ctctccccat
     3001 gagcgctaag aaaatcatcg gccgccgcgg cgctttggaa ttgactgaaa acgctgtcgt
     3061 caacctcggc gtcggtgctc cggaatacgt tgcttctgtt gccggtgaag aaggtatcgc
     3121 cgataccatt accctgaccg tcgaaggtgg cgccatcggt ggcgtaccgc agggcggtgc
     3181 ccgcttcggt tcgtcccgca atgccgatgc catcatcgac cacacctatc agttcgactt
     3241 ctacgatggc ggcggtctgg acatcgctta cctcggcctg gcccagtgcg atggctcggg
     3301 caacatcaac gtcagcaagt tcggtactaa cgttgccggc tgcggcggtt tccccaacat
     3361 ttcccagcag acaccgaatg tttacttctg cggcaccttc acggctggcg gcttgaaaat
     3421 cgctgtcgaa gacggcaaag tcaagatcct ccaggaaggc aaagccaaga agttcatcaa
     3481 agctgtcgac cagatcactt tcaacggttc ctatgcagcc cgcaacggca aacacgttct
     3541 ctacatcaca gaacgctgcg tatttgaact gaccaaagaa ggcttgaaac tcatcgaagt
     3601 cgcaccgggc atcgatattg aaaaagatat cctcgctcac atggacttca agccgatcat
     3661 tgataatccg aaactcatgg atgcccgcct cttccaggac ggtcccatgg gactgaaaaa
     3721 ataaagatct aaagaggaga aactcgagat gagcgaagtt ttagtatcag ctagctatga
     3781 aggctatgag tcaaatagta ttaatttcac agaaataaac aacattgtaa aagagcgatt
     3841 taaaaagatt gatgaagttg agcgcaaaaa aagagctgaa acatttaata aaaagtatga
     3901 agtcacaaaa gagcttgtaa atggacattt acgcgaaatt attatcccaa ggaggctaat
     3961 aaaatctaga gcagcaaacg acgaaaacta cgctgctgct gtttaactgc aggagtcact
     4021 aagggttagt tagttagatt agcagaaagt caaaagcctc cgaccggagg cttttgacta
     4081 aaacttccct tggggttatc attggggctc actcaaaggc ggtaatcaga taaaaaaaat
     4141 ccttagcttt cgctgaggat gatttctgct agagctgtca gaccaagttt acgagctcgc
     4201 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
     4261 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aagcactagg gacagtaaga
     4321 cgggtaagcc tgttgatgat accgctgcct tactgggtgc attagccagt ctgaatgacc
     4381 tgtcacgggg taatccgaag tggccagact ggaaaatcag agggcaggaa ctgctgaaca
     4441 gcaaaaagtc agatagcacc gcatagcaga cccgccataa aacgccctga gaagcccgtg
     4501 acgggctttt cttgtattat gggtagtttc cttgcatgaa tccataaaag gcgcctgtag
     4561 tgccatttac ccccattcac tgccagagcc gtgagtgcag cgaactgaat gtcacgaaaa
     4621 agacggcgac tcaggtgcct gatggtcgga gacaaaagga atattcagcg atttgcccga
     4681 gcttgcgagg gtgctactta agcctttagg gttttaaggt ctgttttgta gaggagcaaa
     4741 cagcgtttgc gacatccttt tgtaatactg cggaactgac taaagtagtg agttatacac
     4801 agggctggga tctattcttt ttatcttttt ttattctttc tttattctat aaattataac
     4861 cacttgaata taaacaaaaa aaacacacaa aggtctagcg gaatttacag agggtctagc
     4921 agaatttaca agttttccag caaaggtcta gcagaattta cagataccca caactcaaag
     4981 gaaaaggaca tgtaattatc attgactagc ccatctcaat tggtatagtg attaaaatca
     5041 cctagaccaa ttgagatgta tgtctgaatt agttgttttc aaagcaaatg aactagcgat
     5101 tagtcgctat gacttaacgg agcatgaaac caagctaatt ttatgctgtg tggcactact
     5161 caaccccacg attgaaaacc ctacaaggaa agaacggacg gtatcgttca cttataacca
     5221 atacgctcag atgatgaaca tcagtaggga aaatgcttat ggtgtattag ctaaagcaac
     5281 cagagagctg atgacgagaa ctgtggaaat caggaatcct ttggttaaag gctttgagat
     5341 tttccagtgg acaaactgtg ccaagttctc aagcgaaaaa ttagaattag tttttagtga
     5401 agggatattg ccttatcttt tccagttaaa aaaattcata aaatataatc tggaacatgt
     5461 taagtctttt gaaaacaaat actctatgag gatttatgag tggttattaa aagaactaac
     5521 acaaaagaaa actcacaagg caaatataga gattagcctt gatgaattta agttcatgtt
     5581 aatgcttgaa aataactacc atgagtttaa aaggcttaac caatgggttt tgaaaccaat
     5641 aagtaaagat ttgaacactt acagcaatat gaaattggtg gttgataagc gaggccgccc
     5701 gactgatacg ttgattttcc aagttgaact agatagacaa atggatctcg taaccgaact
     5761 tgagaacaac cagataaaaa tgaatggtga caaaatacca acaaccatta catcagattc
     5821 ctacctacat aacggactaa gaaaaacact acacgatgct ttaactgcaa aaattcagtt
     5881 caccagtttt gaggcaaaat ttttgggtga catgcaaagt aagtatgatc tcaatggttc
     5941 gttctcatgg ctcacgcaaa aacaacgaac cacactagag aacatactgg ctaaatacgg
     6001 aaggatctga ggttcttatg gctcttgtat ctatcagtga agcatcaaga ctaacaaaca
     6061 aaagtagaac aactgttcac cgttacatat caaagggaaa actgtccata tgcacagatg
     6121 aaaacggtgt aaaaaagata gatacatcag agcttttacg agtttttggt gcattcaaag
     6181 ctgttcacca tgaacagatc gacaatgtaa ctactagagc taaatacatt caaatatcta
     6241 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagaatat
     6301 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6361 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgcc gaagatcagt tgggcgcacg
     6421 tgtgggttac atcgaactgg acctcaacag cggtaagatt cttgagagtt ttcgccccga
     6481 agaacgtttc ccaatgatga gcacttttaa agttctgctc tgtggcgcgg tattatcccg
     6541 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6601 tgagtactca ccagtcacag aaaagcatct tacggacggc atgacagtac gcgaattatg
     6661 cagcgctgcc ataaccacga gtgataacac ggcggccaac ttacttctga caacgatcgg
     6721 gggaccgaag gagcttaccg cttttttgca caacatgggt gatcatgtaa ctcgccttga
     6781 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6841 tgtagctatg gcaacaacgt tgcgcaaact cttaactggc gaacttctta ctctcgcttc
     6901 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcgggaccac ttctgcgctc
     6961 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtcccg
     7021 cggtattatt gcagccctgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7081 gacggggagc caggcaacta tggacgaacg taatcgccag atcgctgaga taggtgcctc
     7141 cctgattaag cattggtaat aatactagct ccggcaaaaa aacgggcaa
//
