LOCUS       62056_19335        4081 bp DNA     circular SYN 07-DEC-2019
DEFINITION  Cloning vector pSBo-2, complete sequence.
ACCESSION   MN436822
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2019) Millennium Institute for Integrative Biology
            - iBio, Portugal 49, Santiago, RM 8331150, Chile
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2019) Millennium Institute for Integrative Biology - iBio, 
            Portugal 49, Santiago, RM 8331150, Chile"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..40
                     /label=UNS1
                     /note="UNS1"
     misc_feature    44..50
                     /label=SapI
                     /note="SapI"
     misc_feature    52..54
                     /label=Beta
                     /note="Beta"
     misc_feature    56..59
                     /label=A
                     /note="A"
     misc_feature    61..66
                     /label=BsaI
                     /note="BsaI"
     protein_bind    131..152
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        167..197
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    205..221
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     229..245
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(258..314)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(315..331)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(666..671)
                     /label=BsaI
                     /note="BsaI"
     misc_feature    673..676
                     /label=F
                     /note="F"
     misc_feature    678..680
                     /label=Gamma
                     /note="Gamma"
     misc_feature    complement(682..688)
                     /label=SapI
                     /note="SapI"
     misc_feature    692..731
                     /label=UNSX
                     /note="UNSX"
     terminator      743..774
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     primer_bind     complement(802..821)
                     /label=VR
                     /note="VR"
     misc_feature    822..862
                     /label=BBa_B0062
                     /note="BBa_B0062"
     CDS             complement(876..1688)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(1689..1836)
                     /label=BBa_P1007 promoter
                     /note="BBa_P1007 promoter"
                     /regulatory_class="promoter"
     CDS             complement(2049..2996)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(3044..3266)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3758..3852)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      3889..3948
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     3961..3980
                     /label=VF2
                     /note="VF2"
     terminator      complement(4008..4037)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_feature    4059..4070
                     /label=STOP all frames
                     /note="STOP all frames"
     misc_feature    4071..4081
                     /label=BBa_B0043
                     /note="BBa_B0043"
ORIGIN
        1 cattactcgc atccattctc aggctgtctc gtctcgtctc cgagctcttc agcaaggagt
       61 gagaccattg acagcgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
      121 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
      181 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
      241 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta
      301 ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      361 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      421 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
      481 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
      541 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
      601 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gacactagtg
      661 agcacggtct cacgctatac tgaagagcac cccaggatac atagattacc acaactccga
      721 gcccttccac cgattagcag aaagtcaaaa gcctccgacc ggaggctttt gactaaaact
      781 tcccttgggg ttatcattgg ggctcactca aaggcggtaa tcagataaaa aaaatcctta
      841 gctttcgcta aggatgattt ctgctagtat tattagaaaa actcatcgag catcaaatga
      901 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
      961 aatgaaggag aaaactcacc gaggcagttc caaagaatgg caaggtcctg gtaacggtct
     1021 gcgattccga cccgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     1081 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caagagcttg
     1141 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     1201 gcatcaacca aaccgttatt catgcgtgat tgcgcctgag caagacgaaa tacacgatcg
     1261 ctgttaaaag gacaattaca aacaggaatc gaatgtaacc ggcgcaggaa cacggccagc
     1321 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa ggctgttttc
     1381 ccaggaatcg cggtggtgag taaccacgca tcatcaggag tacggataaa atgcttgatg
     1441 gtcgggagag gcataaactc cgtcagccag ttgagacgga ccatctcatc tgtaacatca
     1501 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     1561 aagcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     1621 aaatcagcgt ccatgttgga gtttaagcgc ggacgggagc aagacgtttc ccgttgaata
     1681 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
     1741 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac gtggctttgt
     1801 tgaataaatc gaacttttgc tgagttgaag gatcagctct agtagttaca ttgtcgatct
     1861 gttcatggtg aacagcttta aatgcaccaa aaactcgtaa aagctctgat gtatctatct
     1921 tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgatatc taacggtgaa
     1981 cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca agagccataa
     2041 gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg ttgtttttgc
     2101 gtgagccatg agaacgaacc attgagatca tgcttacttt gcatgtcact caaaaatttt
     2161 gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt ttttcttagt
     2221 ccgttacgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc attcattttt
     2281 atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc aacttggaaa
     2341 atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt gctgtaagtg
     2401 tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa ctcatggtag
     2461 ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat atttgccttg
     2521 tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat agagtatttg
     2581 ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa ctggaaaaga
     2641 taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa cttggcatag
     2701 tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc cacagttctc
     2761 gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct actgatgttc
     2821 atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct tgtagggttt
     2881 tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc atgctccgtt
     2941 aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc agacatacat
     3001 ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag tcaatgataa
     3061 ttacatgtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac ctttgctgga
     3121 aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg tgtttttttt
     3181 gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa gataaaaaga
     3241 atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta ttacaaaagg
     3301 atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa aggcttaagt
     3361 agcaccctcg caagctcggg caaatcgctg aatattcctt ttgtctccga ccatcaggca
     3421 cctgagtcgc tgtctttttc gtgacattca gttcgctgcg ctcacggctc tggcagtgaa
     3481 tgggggtaaa tggcactaca ggcgcctttt atggattcat gcaaggaaac tacccataat
     3541 acaagaaaag cccgtcacgg gcttctcagg gcgttttatg gcgggtctgc tatgtggtgc
     3601 tatctgactt tttgctgttc agcagttcct gccctctgat tttccagtct gaccacttcg
     3661 gattatcccg tgacaggtca ttcagactgg ctaatgcacc cagtaaggca gcggtatcat
     3721 caacaggctt acccgtctta ctgtccctag tgcttggatt ctcaccaata aaaaacgccc
     3781 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     3841 tcaacaggag tccaagcgag ctcgtaaact tggtctgaca gctctagctc cggcaaaaaa
     3901 acgggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt
     3961 tgccacctga cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc
     4021 acccgtgaag gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga
     4081 c
//
