LOCUS       62056_19305        3924 bp DNA     circular SYN 07-DEC-2019
DEFINITION  Cloning vector pSBe-2, complete sequence.
ACCESSION   MN436818
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, 
            Bielinski V, von Dassow P, Dupont CL, Federici F.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2019) Millennium Institute for Integrative Biology
            - iBio, Portugal 49, Santiago, RM 8331150, Chile
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2019) Millennium Institute for Integrative Biology - iBio, 
            Portugal 49, Santiago, RM 8331150, Chile"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..40
                     /label=UNS1
                     /note="UNS1"
     misc_feature    complement(44..49)
                     /label=BsaI
                     /note="BsaI"
     misc_feature    51..54
                     /label=B
                     /note="B"
     misc_feature    56..58
                     /label=Alpha
                     /note="Alpha"
     misc_feature    complement(60..66)
                     /label=SapI
                     /note="SapI"
     protein_bind    130..151
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        166..196
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    204..220
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     228..244
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(257..313)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(314..330)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    665..671
                     /label=SapI
                     /note="SapI"
     misc_feature    673..675
                     /label=Omega
                     /note="Omega"
     misc_feature    677..680
                     /label=C
                     /note="C"
     misc_feature    682..687
                     /label=BsaI
                     /note="BsaI"
     misc_feature    691..730
                     /label=UNSX
                     /note="UNSX"
     terminator      742..773
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     misc_feature    799
                     /label=G->T
                     /note="G->T"
     primer_bind     complement(801..820)
                     /label=VR
                     /note="VR"
     misc_feature    821..861
                     /label=BBa_B0062
                     /note="BBa_B0062"
     CDS             complement(875..1531)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     regulatory      complement(1532..1679)
                     /label=BBa_P1007 promoter
                     /note="BBa_P1007 promoter"
                     /regulatory_class="promoter"
     CDS             complement(1892..2839)
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     rep_origin      complement(2887..3109)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3601..3695)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      3732..3791
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     3804..3823
                     /label=VF2
                     /note="VF2"
     terminator      complement(3851..3880)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_feature    3902..3913
                     /label=STOP all frames
                     /note="STOP all frames"
     misc_feature    3914..3924
                     /label=BBa_B0043
                     /note="BBa_B0043"
ORIGIN
        1 cattactcgc atccattctc aggctgtctc gtctcgtctc cgaggtctca tactaatgtg
       61 aagagcttga cagcgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      121 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
      181 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
      241 tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac
      301 cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      361 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      421 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
      481 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
      541 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
      601 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg acactagtga
      661 gcacgctctt caggtaaatg tgagaccacc ccaggataca tagattacca caactccgag
      721 cccttccacc gattagcaga aagtcaaaag cctccgaccg gaggcttttg actaaaactt
      781 cccttggggt tatcattgtg gctcactcaa aggcggtaat cagataaaaa aaatccttag
      841 ctttcgctaa ggatgatttc tgctagtatt attacgcccc gccctgccac tcatcacagt
      901 actgttgtaa ttcattaagc atgcggccga catggaagcc atcacaaacg gcatgatgaa
      961 cctggatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatcgtga
     1021 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     1081 cccagggatt ggctgagaca aaaaacatat tctcaataaa ccctttaggg aaataggcca
     1141 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg gagaaactgc cggaaatcgt
     1201 cgtggtattc actccagagg gacgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     1261 aagggtgaac actatcccag atcaccagct caccgtcttt catggccata cgaaactccg
     1321 ggtgagcgtt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     1381 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     1441 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     1501 cggtggtata tcccgtgatt tttttctcca taacacccct tgtattactg tttatgtaag
     1561 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     1621 tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcagc
     1681 tctagtagtt acattgtcga tctgttcatg gtgaacagct ttaaatgcac caaaaactcg
     1741 taaaagctct gatgtatcta tcttttttac accgttttca tctgtgcata tggacagttt
     1801 tccctttgat atctaacggt gaacagttgt tctacttttg tttgttagtc ttgatgcttc
     1861 actgatagat acaagagcca taagaacctc agatccttcc gtatttagcc agtatgttct
     1921 ctagtgtggt tcgttgtttt tgcgtgagcc atgagaacga accattgaga tcatgcttac
     1981 tttgcatgtc actcaaaaat tttgcctcaa aactggtgag ctgaattttt gcagttaaag
     2041 catcgtgtag tgtttttctt agtccgttac gtaggtagga atctgatgta atggttgttg
     2101 gtattttgtc accattcatt tttatctggt tgttctcaag ttcggttacg agatccattt
     2161 gtctatctag ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc ttatcaacca
     2221 ccaatttcat attgctgtaa gtgtttaaat ctttacttat tggtttcaaa acccattggt
     2281 taagcctttt aaactcatgg tagttatttt caagcattaa catgaactta aattcatcaa
     2341 ggctaatctc tatatttgcc ttgtgagttt tcttttgtgt tagttctttt aataaccact
     2401 cataaatcct catagagtat ttgttttcaa aagacttaac atgttccaga ttatatttta
     2461 tgaatttttt taactggaaa agataaggca atatctcttc actaaaaact aattctaatt
     2521 tttcgcttga gaacttggca tagtttgtcc actggaaaat ctcaaagcct ttaaccaaag
     2581 gattcctgat ttccacagtt ctcgtcatca gctctctggt tgctttagct aatacaccat
     2641 aagcattttc cctactgatg ttcatcatct gagcgtattg gttataagtg aacgataccg
     2701 tccgttcttt ccttgtaggg ttttcaatcg tggggttgag tagtgccaca cagcataaaa
     2761 ttagcttggt ttcatgctcc gttaagtcat agcgactaat cgctagttca tttgctttga
     2821 aaacaactaa ttcagacata catctcaatt ggtctaggtg attttaatca ctataccaat
     2881 tgagatgggc tagtcaatga taattacatg tccttttcct ttgagttgtg ggtatctgta
     2941 aattctgcta gacctttgct ggaaaacttg taaattctgc tagaccctct gtaaattccg
     3001 ctagaccttt gtgtgttttt tttgtttata ttcaagtggt tataatttat agaataaaga
     3061 aagaataaaa aaagataaaa agaatagatc ccagccctgt gtataactca ctactttagt
     3121 cagttccgca gtattacaaa aggatgtcgc aaacgctgtt tgctcctcta caaaacagac
     3181 cttaaaaccc taaaggctta agtagcaccc tcgcaagctc gggcaaatcg ctgaatattc
     3241 cttttgtctc cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct
     3301 gcgctcacgg ctctggcagt gaatgggggt aaatggcact acaggcgcct tttatggatt
     3361 catgcaagga aactacccat aatacaagaa aagcccgtca cgggcttctc agggcgtttt
     3421 atggcgggtc tgctatgtgg tgctatctga ctttttgctg ttcagcagtt cctgccctct
     3481 gattttccag tctgaccact tcggattatc ccgtgacagg tcattcagac tggctaatgc
     3541 acccagtaag gcagcggtat catcaacagg cttacccgtc ttactgtccc tagtgcttgg
     3601 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
     3661 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgtaa acttggtctg
     3721 acagctctag ctccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa
     3781 aaccgaaaag attacttcgc gtttgccacc tgacgtctaa gaaaaggaat attcagcaat
     3841 ttgcccgtgc cgaagaaagg cccacccgtg aaggtgagcc agtgagttga ttgctacgta
     3901 attagttagt tagcccttag tgac
//
