LOCUS       40924_38803        5751 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSBFLPM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Bowden SD, Palani NP, Libourel IG.
  TITLE     Stringent control of FLP recombinase in Escherichia coli
  JOURNAL   J. Microbiol. Methods 133, 52-54 (2016)
  PUBMED    28024983
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Bowden SD, Palani NP, Libourel IG.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2016) Biotechnology Institute, University of 
            Minnesota, 1500 Gortner Avenue, Saint Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 5751)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5751)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Microbiol. Methods 133, 52-54 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-DEC-2016) Biotechnology Institute, University of Minnesota, 1500
            Gortner Avenue, Saint Paul, MN 55108, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /label=AmpR promoter
     CDS             132..989
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      996..1035
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     oriT            1182..1290
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      1841..2063
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2111..3058
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
     CDS             complement(3256..4524)
                     /label=FLP
                     /note="site-specific recombinase"
     regulatory      complement(4531..4536)
                     /regulatory_class="ribosome_binding_site"
     promoter        complement(4552..4836)
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             4863..5738
                     /label=araC
                     /note="L-arabinose regulatory protein"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagcatt cagcattttc gtgtggcgct gattccgttt tttgcggcgt
      181 tttgcctgcc ggtgtttgcg catccggaaa ccctggtgaa agtgaaagat gcggaagatc
      241 aactgggtgc gcgcgtgggc tatattgaac tggatctgaa cagcggcaaa attctggaat
      301 cttttcgtcc ggaagaacgt tttccgatga tgagcacctt taaagtgctg ctgtgcggtg
      361 cggttctgag ccgtgtggat gcgggccagg aacaactggg ccgtcgtatt cattatagcc
      421 agaacgatct ggtggaatat agcccggtga ccgaaaaaca tctgaccgat ggcatgaccg
      481 tgcgtgaact gtgcagcgcg gcgattacca tgagcgataa caccgcggcg aacctgctgc
      541 tgacgaccat tggcggtccg aaagaactga ccgcgtttct gcataacatg ggcgatcatg
      601 tgacccgtct ggatcgttgg gaaccggaac tgaacgaagc gattccgaac gatgaacgtg
      661 ataccaccat gccggcagca atggcgacca ccctgcgtaa actgctgacg ggtgagctgc
      721 tgaccctggc aagccgccag caactgattg attggatgga agcggataaa gtggcgggtc
      781 cgctgctgcg tagcgcgctg ccggctggct ggtttattgc ggataaaagc ggtgcgggcg
      841 aacgtggcag ccgtggcatt attgcggcgc tgggcccgga tggtaaaccg agccgtattg
      901 tggtgattta taccaccggc agccaggcga cgatggatga acgtaaccgt cagattgcgg
      961 aaattggcgc gagcctgatt aaacattggt aaaccgatac aattaaaggc tccttttgga
     1021 gccttttttt ttggacgacc cttgtccttt tccgctgcat aaccctgctt cggggtcatt
     1081 atagcgattt tttcggtata tccatccttt ttcgcacgat atacaggatt ttgccaaagg
     1141 gttcgtgtag actttccttg gtgtatccaa cggcgtcagc cgggcaggat aggtgaagta
     1201 ggcccacccg cgagcgggtg ttccttcttc actgtccctt attcgcacct ggcggtgctc
     1261 aacgggaatc ctgctctgcg aggctggccg taggccggcc ggcgcgccga tctgaagatc
     1321 agcagttcaa cctgttgagt tttcgttcca ctgagcgtca gaccccgttg atgataccgc
     1381 tgccttactg ggtgcattag ccagtctgaa tgacctgtca cgggataatc cgaagtggtc
     1441 agactggaaa atcagagggc aggaactgct gaacagcaaa aagtcagata gcaccacata
     1501 gcagacccgc cataaaacgc cctgagaagc ccgtgacggg cttttcttgt attatgggta
     1561 gtttccttgc atgaatccat aaaaggcgcc tgtagtgcca tttaccccca ttcactgcca
     1621 gagccgtgag cgcagcgaac tgaatgtcac gaaaaagaca gcgactcagg tgcctgatgg
     1681 tcggagacaa aaggaatatt cagcgatttg cccgattgcg gccgcaaccg agcttgcgag
     1741 ggtgctactt aagcctttag ggttttaagg tctgttttgt agaggagcaa acagcgtttg
     1801 cgacatcctt ttgtaatact gcggaactga ctaaagtagt gagttataca cagggctggg
     1861 atctattctt tttatctttt tttattcttt ctttattcta taaattataa ccacttgaat
     1921 ataaacaaaa aaaacacaca aaggtctagc ggaatttaca gagggtctag cagaatttac
     1981 aagttttcca gcaaaggtct agcagaattt acagataccc acaactcaaa ggaaaaggac
     2041 tagtaattat cattgactag cccatctcaa ttggtatagt gattaaaatc acctagacca
     2101 attgagatgt atgtctgaat tagttgtttt caaagcaaat gaactagcga ttagtcgcta
     2161 tgacttaacg gagcatgaaa ccaagctaat tttatgctgt gtggcactac tcaaccccac
     2221 gattgaaaac cctacaagga aagaacggac ggtatcgttc acttataacc aatacgctca
     2281 gatgatgaac atcagtaggg aaaatgctta tggtgtatta gctaaagcaa ccagagagct
     2341 gatgacgaga actgtggaaa tcaggaatcc tttggttaaa ggctttgaga ttttccagtg
     2401 gacaaactat gccaagttct caagcgaaaa attagaatta gtttttagtg aagagatatt
     2461 gccttatctt ttccagttaa aaaaattcat aaaatataat ctggaacatg ttaagtcttt
     2521 tgaaaacaaa tactctatga ggatttatga gtggttatta aaagaactaa cacaaaagaa
     2581 aactcacaag gcaaatatag agattagcct tgatgaattt aagttcatgt taatgcttga
     2641 aaataactac catgagttta aaaggcttaa ccaatgggtt ttgaaaccaa taagtaaaga
     2701 tttaaacact tacagcaata tgaaattggt ggttgataag cgaggccgcc cgactgatac
     2761 gttgattttc caagttgaac tagatagaca aatggatctc gtaaccgaac ttgagaacaa
     2821 ccagataaaa atgaatggtg acaaaatacc aacaaccatt acatcagatt cctacctacg
     2881 taacggacta agaaaaacac tacacgatgc tttaactgca aaaattcagc tcaccagttt
     2941 tgaggcaaaa tttttgagtg acatgcaaag taagtatgat ctcaatggtt cgttctcatg
     3001 gctcacgcaa aaacaacgaa ccacactaga gaacatactg gctaaatacg gaaggatctg
     3061 aggttcttat ggctcttgta tctatcagtg aagcatcaag actaacaaac aaaagtagaa
     3121 caactgttca ccgttacata tcaaagggaa aactgtccat acccatggat tcttcgtctg
     3181 tttctactgg tattggcaca aacctgattc caatttgagc aaggctatgt gccatctcga
     3241 tactcgtcta gattatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg
     3301 tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg
     3361 ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt
     3421 tgatattgga tcatatgcat agtaccgaga aactagtgcg aagtagtgat caggtattgc
     3481 tgttatctga tgagtatacg ttgtcctggc cacggcagaa gcacgcttat cgctccaatt
     3541 tcccacaaca ttagtcaact ccgttaggcc cttcattgaa agaaatgagg tcatcaaatg
     3601 tcttccaatg tgagattttg ggccattttt tatagcaaag attgaataag gcgcattttt
     3661 cttcaaagct ttattgtacg atctgactaa gttatctttt aataattggt attcctgttt
     3721 attgcttgaa gaattgccgg tcctatttac tcgttttagg actggttcag aattcctcaa
     3781 aaattcatcc aaatatacaa gtggatcgat cctacccctt gcgctaaaga agtatatgtg
     3841 cctactaacg cttgtctttg tctctgtcac taaacactgg attattactc ccagatactt
     3901 attttggact aatttaaatg atttcggatc aacgttctta atatcgctga atcttccaca
     3961 attgatgaaa gtagctagga agaggaattg gtataaagtt tttgtttttg taaatctcga
     4021 agtatactca aacgaattta gtattttctc agtgatctcc cagatgcttt caccctcact
     4081 tagaagtgct ttaagcattt ttttactgtg gctatttccc ttatctgctt cttccgatga
     4141 ttcgaactgt aattgcaaac tacttacaat atcagtgata tcagattgat gtttttgtcc
     4201 atagtaagga ataattgtaa attcccaagc aggaatcaat ttctttaatg aggcttccag
     4261 aattgttgct ttttgcgtct tgtatttaaa ctggagtgat ttattgacaa tatcgaaact
     4321 cagcgaattg cttatgatag tattatagct catgaatgtg gctctcttga ttgctgttcc
     4381 gttatgtgta atcatccaac ataaataggt tagttcagca gcacataatg ctattttctc
     4441 acctgaaggt ctttcaaacc tttccacaaa ctgacgaaca agcaccttag gtggtgtttt
     4501 acataatata tcaaattgtg gcatcgtttc actccatcca aaaaaacggg tatggagaaa
     4561 cagtagagag ttgcgataaa aagcgtcagg taggatccgc taatcttatg gataaaaatg
     4621 ctatggcata gcaaagtgtg acgccgtgca aataatcaat gtggactttt ctgccgtgat
     4681 tatagacact tttgttacgc gtttttgtca tggctttggt cccgctttgt tacagaatgc
     4741 ttttaataag cggggttacc ggtttggtta gcgagaagag ccagtaaaag acgcagtgac
     4801 ggcaatgtct gatgcaatat ggacaattgg tttcttctct gaatggcggg agtatgaaaa
     4861 gtatggctga agcgcaaaat gatcccctgc tgccgggata ctcgtttaat gcccatctgg
     4921 tggcgggttt aacgccgatt gaggccaacg gttatctcga tttttttatc gaccgaccgc
     4981 tgggaatgaa aggttatatt ctcaatctca ccattcgcgg tcagggggtg gtgaaaaatc
     5041 agggacgaga atttgtttgc cgaccgggtg atattttgct gttcccgcca ggagagattc
     5101 atcactacgg tcgtcatccg gaggctcgcg aatggtatca ccagtgggtt tactttcgtc
     5161 cgcgcgccta ctggcatgaa tggcttaact ggccgtcaat atttgccaat acggggttct
     5221 ttcgcccgga tgaagcgcac cagccgcatt tcagcgacct gtttgggcaa atcattaacg
     5281 ccgggcaagg ggaagggcgc tattcggagc tgctggcgat aaatctgctt gagcaattgt
     5341 tactgcggcg catggaagcg attaacgagt cgctccatcc accgatggat aatcgggtac
     5401 gcgaggcttg tcagtacatc agcgatcacc tggcagacag caattttgat atcgccagcg
     5461 tcgcacagca tgtttgcttg tcgccgtcgc gtctgtcaca tcttttccgc cagcagttag
     5521 ggattagcgt cttaagctgg cgcgaggacc aacgtatcag ccaggcgaag ctgcttttga
     5581 gcaccacccg gatgcctatc gccaccgtcg gtcgcaatgt tggttttgac gatcaactct
     5641 atttctcgcg ggtatttaaa aaatgcaccg gggccagccc gagcgagttc cgtgccggtt
     5701 gtgaagaaaa agtgaatgat gtagccgtca agttgtcata ataaatcgat g
//
