LOCUS       62056_19320        4881 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             158..1807
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(1851..1972)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2391..2979)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3153..4010)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4011..4115)
                     /label=AmpR promoter
     rep_origin      4142..4597
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4728..4776
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4790..4881
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 atcgataggt accgagctcg gagtatgtct agactgacaa tgctagcccg ggctcgacag
       61 ctggacgtcg atatcgaatt cgggtatata atggatccgg tatcgagatc tgcgatctaa
      121 gtaagcttgg cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa
      181 gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa
      241 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
      301 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa
      361 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
      421 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
      481 ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt
      541 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa
      601 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt
      661 cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag
      721 ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt
      781 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg
      841 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
      901 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
      961 tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt
     1021 gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa
     1081 tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc
     1141 caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc
     1201 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
     1261 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg
     1321 cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac
     1381 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
     1441 agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca
     1501 ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg
     1561 tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
     1621 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
     1681 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
     1741 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat
     1801 cgccgtgtaa ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat
     1861 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     1921 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     1981 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     2041 gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg cccttgagag
     2101 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     2161 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc
     2221 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2281 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2341 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2401 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2461 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2521 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2581 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2641 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2701 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2761 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2821 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2881 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2941 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3001 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3061 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3121 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3181 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3241 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3301 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3361 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3421 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3481 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3541 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3601 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     3661 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3721 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3781 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3841 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3901 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3961 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4021 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4081 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4141 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4201 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4261 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4321 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4381 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4441 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4501 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4561 aacgcgaatt ttaacaaaat attaacgctt acaatttgcc attcgccatt caggctgcgc
     4621 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca
     4681 tgataagtaa gtaatattaa ggtacgggag gtacttggag cggccgcaat aaaatatctt
     4741 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
     4801 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
     4861 caggtgccag aacatttctc t
//
