LOCUS       40924_38753        4342 bp DNA     circular SYN 18-DEC-2018
DEFINITION  BioBrick cloning vector pSB3T5-I52001, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Shetty RP, Endy D, Knight TF Jr.
  TITLE     Engineering BioBrick vectors from BioBrick parts
  JOURNAL   J Biol Eng 2, 5 (2008)
  PUBMED    18410688
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Shetty RP, Endy D, Knight TF Jr.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2008) Biological Engineering, MIT, 32 Vassar 
            Street Room 32-311, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 4342)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4342)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol Eng 
            2, 5 (2008)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-FEB-2008) Biological Engineering, MIT, 32 Vassar Street Room 
            32-311, Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..21
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    35..46
                     /note="translational stop sequence for BioBrick
                     vector-insert insulation, has stop codons in all 6 reading 
                     frames (BBa_B0042)"
     terminator      57..88
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     primer_bind     complement(116..135)
                     /note="VR reverse sequencing primer annealing site
                     (BBa_G00102)"
     regulatory      136..176
                     /note="derived from ribosomal RNA rrnC operon, reverse 
                     orientation (BBa_B0062); similar to Escherichia coli strain
                     MG1655 accession U00096"
                     /regulatory_class="terminator"
     stem_loop       complement(149..168)
                     /label=derived from ribosomal RNA rrnC operon
                     /note="derived from ribosomal RNA rrnC operon"
     rep_origin      599..1143
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        1759..1787
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             1835..3022
                     /label=TcR
                     /note="tetracycline efflux protein"
     terminator      3036..3095
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     3108..3127
                     /note="VF2 forward sequencing primer annealing site 
                     (BBa_G00100)"
     terminator      complement(3155..3184)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_feature    3206..3217
                     /note="translational stop sequence for BioBrick
                     vector-insert insulation, has stop codons in all 6 reading 
                     frames (BBa_B0042)"
     misc_feature    3231..3252
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     CDS             3360..3662
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     rep_origin      3695..4283
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc
       61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca
      121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta
      181 gagatggaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      241 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
      301 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
      361 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
      421 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
      481 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
      541 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cttaataaga tgatcttctt
      601 gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg
      661 gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag
      721 gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga
      781 ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc
      841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt
      901 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga
      961 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg
     1021 agagcgcacg agggagccgc caggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1081 cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg
     1141 aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca
     1201 ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta
     1261 gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg
     1321 gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac
     1381 atagtaagcc agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga aggtgttgct
     1441 gactcatacc aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg
     1501 atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa
     1561 cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt
     1621 attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     1681 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgtttac
     1741 tagaggagat tctcatgttt gacagcttat catcgataag ctttaatgcg gtagtttatc
     1801 acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt
     1861 cattctcggc accgtcaccc tggacgctgt aggcataggc ttggttatgc cggtactgcc
     1921 gggcctcttg cgggatatcg tccattccga cagtattgcc agtcactatg gcgtgctgct
     1981 tgcgctctat gcgttgatgc aatttctttg cgcacccgtt ctcggagccc tgtccgaccg
     2041 ctttggccgc cgtccagtcc tgctcgcttc gctccttgga gccactatcg actacgcgat
     2101 catggcgacc acacccgtcc tgtggattct ctacgccgga cgcatcgtgg cgggcatcac
     2161 gggtgccaca ggtgcggttg ctggtgccta tatcgccgac atcaccgatg gggaagatcg
     2221 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
     2281 ggccggggga ctgttgggtg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
     2341 caacggcctc aacctcctcc tgggctgctt ccttatgcag gaatcgcata agggagagcg
     2401 ccgtccgatg cccttgcgtg ccttcaatcc agtcagctcc ttccggtggg cgcggggcat
     2461 gactatcgtc gccgcactta tgactgtttt ctttatcatg caactcgtag gacaggttcc
     2521 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg
     2581 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcacgggccc
     2641 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcg ggcatggcgg ccgacgcgct
     2701 gggctacgtc ttgctggcgt tcgcgacgcg cggctggatg gccttcccca ttatgattct
     2761 tctcgcttcc ggcggcatcg gtatgcccgc gttgcaggcc atgctgtccc gccaagtaga
     2821 tgacgaccat cagggacagc ttcaagggtc gctcgcggct cttaccagcc tcacttcgat
     2881 cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt
     2941 ggcatggatt gtaggtgccg ccctttacct tgtctgcctc cccgcgttgc gtcgcggtgc
     3001 atggagccgg gccacctcga cctaataata ctagctccgg caaaaaaacg ggcaaggtgt
     3061 caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgtttgc cacctgacgt
     3121 ctaagaaaag gaatattcag caatttgccc gtgccgaaga aaggcccacc cgtgaaggtg
     3181 agccagtgag ttgattgcta cgtaattagt tagttagccc ttagtgactc gaattcgcgg
     3241 ccgcttctag agggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt
     3301 tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtatgcta
     3361 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     3421 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     3481 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     3541 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     3601 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     3661 tataataacc cgtagaaaag atcaaaagat cttcttgaga tccttttttt ctgcgcgtaa
     3721 tctgctactt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3781 agctaccaac tctttttccg aaggtaactg gctttagcag agcgcagata ccaaatactg
     3841 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3901 acctcgctct gctaatcctg ttaccagtag ctactgccag tagcgataag tcgtgtctta
     3961 ccgggttgga ttcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4021 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4081 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatctggtaa
     4141 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4201 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4261 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4321 tttgctggcc ttttgctcac at
//
