LOCUS       40924_38743        3828 bp DNA     circular SYN 18-DEC-2018
DEFINITION  BioBrick cloning vector pSB3C5-I52001, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Shetty RP, Endy D, Knight TF Jr.
  TITLE     Engineering BioBrick vectors from BioBrick parts
  JOURNAL   J Biol Eng 2, 5 (2008)
  PUBMED    18410688
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Shetty RP, Endy D, Knight TF Jr.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2008) Biological Engineering, MIT, 32 Vassar 
            Street Room 32-311, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 3828)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3828)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol Eng 
            2, 5 (2008)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-FEB-2008) Biological Engineering, MIT, 32 Vassar Street Room 
            32-311, Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..21
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    35..46
                     /note="translational stop sequence for BioBrick
                     vector-insert insulation, has stop codons in all 6 reading 
                     frames (BBa_B0042)"
     terminator      57..88
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     primer_bind     complement(116..135)
                     /note="VR reverse sequencing primer annealing site
                     (BBa_G00102)"
     regulatory      136..176
                     /note="derived from ribosomal RNA rrnC operon, reverse 
                     orientation (BBa_B0062); similar to Escherichia coli strain
                     MG1655 accession U00096"
                     /regulatory_class="terminator"
     stem_loop       complement(149..168)
                     /label=derived from ribosomal RNA rrnC operon
                     /note="derived from ribosomal RNA rrnC operon"
     rep_origin      599..1143
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        1748..1851
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1852..2508
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      2522..2581
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     2594..2613
                     /note="VF2 forward sequencing primer annealing site 
                     (BBa_G00100)"
     terminator      complement(2641..2670)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     misc_feature    2692..2703
                     /note="translational stop sequence for BioBrick
                     vector-insert insulation, has stop codons in all 6 reading 
                     frames (BBa_B0042)"
     misc_feature    2717..2738
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      2779..2784
                     /gene="ccdB"
                     /regulatory_class="minus_35_signal"
     regulatory      2802..2807
                     /gene="ccdB"
                     /regulatory_class="minus_10_signal"
     CDS             2846..3148
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     rep_origin      3181..3769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc
       61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca
      121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta
      181 gagatggaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
      241 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
      301 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
      361 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
      421 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
      481 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
      541 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cttaataaga tgatcttctt
      601 gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg
      661 gcggtttttc gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag
      721 gagcgcagtc accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga
      781 ctaactcctc taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc
      841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt
      901 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga
      961 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg
     1021 agagcgcacg agggagccgc caggggaaac gcctggtatc tttatagtcc tgtcgggttt
     1081 cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg gagcctatgg
     1141 aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg gcatcttcca
     1201 ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg accgagcgta
     1261 gcgagtcagt gagcgaggaa gcggaatata tcctgtatca catattctgc tgacgcaccg
     1321 gtgcagcctt ttttctcctg ccacatgaag cacttcactg acaccctcat cagtgccaac
     1381 atagtaagcc agtatacact ccgctagcgc tgaggtctgc ctcgtgaaga aggtgttgct
     1441 gactcatacc aggcctgaat cgccccatca tccagccaga aagtgaggga gccacggttg
     1501 atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt tgccacggaa
     1561 cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt
     1621 attcaacaaa gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     1681 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgtttac
     1741 tagaggttga tcgggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta
     1801 ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatggagaaa
     1861 aaaatcacgg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     1921 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     1981 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     2041 gcccgcctga tgaacgctca cccggagttt cgtatggcca tgaaagacgg tgagctggtg
     2101 atctgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttcg
     2161 tccctctgga gtgaatacca cgacgatttc cggcagtttc tccacatata ttcgcaagat
     2221 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     2281 tttgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     2341 gacaacttct tcgcccccgt tttcacgatg ggcaaatatt atacgcaagg cgacaaggtg
     2401 ctgatgccgc tggcgatcca ggttcatcat gccgtttgtg atggcttcca tgtcggccgc
     2461 atgcttaatg aattacaaca gtactgtgat gagtggcagg gcggggcgta ataatactag
     2521 ctccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
     2581 attacttcgc gtttgccacc tgacgtctaa gaaaaggaat attcagcaat ttgcccgtgc
     2641 cgaagaaagg cccacccgtg aaggtgagcc agtgagttga ttgctacgta attagttagt
     2701 tagcccttag tgactcgaat tcgcggccgc ttctagaggg cttactaaaa gccagataac
     2761 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
     2821 gaagtatgtc aaaaagaggt atgctatgca gtttaaggtt tacacctata aaagagagag
     2881 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
     2941 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
     3001 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
     3061 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
     3121 cgccattaac ctgatgttct ggggaatata ataacccgta gaaaagatca aaagatcttc
     3181 ttgagatcct ttttttctgc gcgtaatctg ctacttgcaa acaaaaaaac caccgctacc
     3241 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3301 tagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     3361 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtagctac
     3421 tgccagtagc gataagtcgt gtcttaccgg gttggattca agacgatagt taccggataa
     3481 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3541 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3601 gagaaaggcg gacaggtatc tggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3661 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3721 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3781 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacat
//
