LOCUS       V013016                 2263 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013016
VERSION     V013016
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..20
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that start with 'ATG'"
     CDS             21..209
                     /gene="CECB1"
                     /label="Cecropin-B"
                     /note="Cecropin-B from Bombyx mori. Accession#: P04142"
     misc_feature    216..236
                     /label="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      237..294
                     /label="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during
                     synthesis. Its efficiency has not been determined."
     rep_origin      complement(489..1077)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(1260..1354)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(1378..2034)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2035..2138)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(2218..2261)
                     /label="bacterial terminator"
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag atgaacttcg ctaaaatcct gtctttcgtt ttcgctctgg
       61 ttctggctct gtctatgacc tctgctgctc cggaaccgcg ttggaaaatc ttcaaaaaaa
      121 tcgaaaaaat gggtcgtaac atccgtgacg gtatcgttaa agctggtccg gctatcgaag
      181 ttctgggttc tgctaaagct atcggtaaat aataatacta gtagcggccg ctgcagtccg
      241 gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa aagattactt
      301 cgcgttatgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      361 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      421 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      481 ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      541 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      601 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      661 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      721 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      781 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      841 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      901 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      961 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1021 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1081 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1141 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1201 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagctc gaggcttgga
     1261 ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt
     1321 tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc
     1381 cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa
     1441 gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg
     1501 cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
     1561 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
     1621 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
     1681 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
     1741 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
     1801 tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
     1861 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
     1921 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
     1981 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
     2041 ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc
     2101 attatggtga aagttggaac ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct
     2161 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt
     2221 tcagataaaa aaaatcctta gctttcgcta aggatgattt ctg
//
