LOCUS       pSB11.        6323 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Superbinary intermediate vector for plant cell transformation, with 
            a spectinomycin-resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    pSB11
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Komari T, Hiei Y, Saito Y, Murai N, Kumashiro T.
  TITLE     Vectors carrying two separate T-DNAs for co-transformation of higher
            plants mediated by Agrobacterium tumefaciens and segregation of 
            transformants free from selection markers.
  JOURNAL   Plant J. 1996;10:165-74.
  PUBMED    8758986
REFERENCE   2  (bases 1 to 6323)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6323)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "1996"; volume: "10"; pages: "165-74"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Homologous recombination with the acceptor vector occurs in the 
            region spanning the cos and bom sites.
FEATURES             Location/Qualifiers
     source          1..6323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..51
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    complement(104..128)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(1304..2092)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase"
                     /label=aadA
                     /note="SmR"
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(2150..2674)
                     /codon_start=1
                     /product="streptothricin acetyltransferase"
                     /label=streptothricin acetyltransferase
                     /note="streptothricin acetyltransferase"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSPY
                     RKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAHSL
                     IEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMY
                     WYWFSGAQDDA"
     misc_feature    3073..3213
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3399..3987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4495..4893
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     promoter        complement(5456..5560)
                     /label=AmpR promoter
     misc_feature    complement(6147..6171)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctcgagtc tagaggatcc ccgggtaccg agctcgaatt cagtacatta
       61 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
      121 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
      181 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
      241 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
      301 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
      361 tgatcaaata tcatctccct cgcagagatc cgaattatca gccttcttat tcatttctcg
      421 cttaaccgtg acaggctgtc gatcttgaga actatgccga cataatagga aatcgctgga
      481 taaagccgct gaggaagctg agtggcgcta tttctttaga agtgaacgtt gacgatcgtc
      541 gaccgtaccc cgatgaatta attcggacgt acgttctgaa cacagctgga tacttacttg
      601 ggcgattgtc atacatgaca tcaacaatgt acccgtttgt gtaaccgtct cttggaggtt
      661 cgtatgacac tagtggttcc cctcagcttg cgactagatg ttgaggccta acattttatt
      721 agagagcagg ctagttgctt agatacatga tcttcaggcc gttatctgtc agggcaagcg
      781 aaaattggcc atttatgacg accaatgccc cgcagaagct cccatctttg ccgccataga
      841 cgccgcgccc cccttttggg gtgtagaaca tccttttgcc agatgtggaa aagaagttcg
      901 ttgtcccatt gttggcaatg acgtagtagc cggcgaaagt gcgagaccca tttgcgctat
      961 atataagcct acgatttccg ttgcgactat tgtcgtaatt ggatgaacta ttatcgtagt
     1021 tgctctcaga gttgtcgtaa tttgatggac tattgtcgta attgcttatg gagttgtcgt
     1081 agttgcttgg agaaatgtcg tagttggatg gggagtagtc atagggaaga cgagcttcat
     1141 ccactaaaac aattggcagg tcagcaagtg cctgccccga tgccatcgca agtacgaggc
     1201 ttagaaccac cttcaacaga tcgcgcatag tcttccccag ctctctaacg cttgagttaa
     1261 gccgcgccgc gaagcggcgt cggcttgaac gaattgttag acattatttg ccgactacct
     1321 tggtgatctc gcctttcacg tagtgaacaa attcttccaa ctgatctgcg cgcgaggcca
     1381 agcgatcttc ttgtccaaga taagcctgcc tagcttcaag tatgacgggc tgatactggg
     1441 ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta
     1501 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt
     1561 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag
     1621 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg
     1681 cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa
     1741 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg
     1801 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc
     1861 caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa
     1921 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca
     1981 ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc
     2041 caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc atgatgttta
     2101 actcctgaat taagccgcgc cgcgaagcgg tgtcggcttg aatgaattgt taggcgtcat
     2161 cctgtgctcc cgagaaccag taccagtaca tcgctgtttc gttcgagact tgaggtctag
     2221 ttttatacgt gaacaggtca atgccgccga gagtaaagcc acattttgcg tacaaattgc
     2281 aggcaggtac attgttcgtt tgtgtctcta atcgtatgcc aaggagctgt ctgcttagtg
     2341 cccacttttt cgcaaattcg atgagactgt gcgcgactcc tttgcctcgg tgcgtgtgcg
     2401 acacaacaat gtgttcgata gaggctagat cgttccatgt tgagttgagt tcaatcttcc
     2461 cgacaagctc ttggtcgatg aatgcgccat agcaagcaga gtcttcatca gagtcatcat
     2521 ccgagatgta atccttccgg taggggctca cacttctggt agatagttca aagccttggt
     2581 cggataggtg cacatcgaac acttcacgaa caatgaaatg gttctcagca tccaatgttt
     2641 ccgccacctg ctcagggatc accgaaatct tcatatgacg cctaacgcct ggcacagcgg
     2701 atcgcaaacc tggcgcggct tttggcacaa aaggcgtgac aggtttgcga atccgttgct
     2761 gccacttgtt aacccttttg ccagatttgg taactataat ttatgttaga ggcgaagtct
     2821 tgggtaaaaa ctggcctaaa attgctgggg atttcaggaa agtaaacatc accttccggc
     2881 tcgatgtcta ttgtagatat atgtagtgta tctacttgat cgggggatct gctgcctcgc
     2941 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     3001 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     3061 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
     3121 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     3181 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     3241 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3301 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3361 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3421 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3481 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3541 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3601 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3661 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3721 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3781 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     3841 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3901 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3961 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4021 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4081 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4141 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4201 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4261 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     4321 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4381 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     4441 gttaatagtt tgcgcaacgt tgttgccatt gctgcagggg gggggggggg ggggttccat
     4501 tgttcattcc acggacaaaa acagagaaag gaaacgacag aggccaaaaa gctcgctttc
     4561 agcacctgtc gtttcctttc ttttcagagg gtattttaaa taaaaacatt aagttatgac
     4621 gaagaagaac ggaaacgcct taaaccggaa aattttcata aatagcgaaa acccgcgagg
     4681 tcgccgcccc gtaacctgtc ggatcaccgg aaaggacccg taaagtgata atgattatca
     4741 tctacatatc acaacgtgcg tggaggccat caaaccacgt caaataatca attatgacgc
     4801 aggtatcgta ttaattgatc tgcatcaact taacgtaaaa acaacttcag acaatacaaa
     4861 tcagcgacac tgaatacggg gcaacctcat gtcccccccc ccccccccct gcaggcatcg
     4921 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     4981 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     5041 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     5101 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     5161 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     5221 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     5281 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     5341 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     5401 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     5461 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     5521 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     5581 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     5641 cgaggccctt tcgtcttcaa gaattggtcg acgatcttgc tgcgttcgga tattttcgtg
     5701 gagttcccgc cacagacccg gattgaaggc gagatccagc aactcgcgcc agatcatcct
     5761 gtgacggaac tttggcgcgt gatgactggc caggacgtcg gccgaaagag cgacaagcag
     5821 atcacgcttt tcgacagcgt cggatttgcg atcgaggatt tttcggcgct gcgctacgtc
     5881 cgcgaccgcg ttgagggatc aagccacagc agcccactcg accttctagc cgacccagac
     5941 gagccaaggg atctttttgg aatgctgctc cgtcgtcagg ctttccgacg tttgggtggt
     6001 tgaacagaag tcattatcgc acggaatgcc aagcactccc gaggggaacc ctgtggttgg
     6061 catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta
     6121 ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat
     6181 agtttaaact gaaggcggga aacgacaatc tgatcatgag cggagaatta agggagtcac
     6241 gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact gacagaaccg
     6301 caacgttgaa ggagccactc agc
//
