LOCUS       62056_19235        3894 bp DNA     circular SYN 16-AUG-2021
DEFINITION  Cloning vector pSA_HQN, complete sequence.
ACCESSION   MZ198785
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Groseclose TM, Hersey AN, Huang BD, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (13-MAY-2021) Chemical "
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        596..673
                     /label=lacI promoter
     CDS             674..1720
                     /codon_start=1
                     /transl_table=11
                     /product="SA_HQN"
                     /label=SA_HQN
                     /protein_id="QYJ58364.1"
                     /translation="MKPVTLYDVAEYAGVSHQTVSNVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAAQLAGKQSDTIGVVVMDVTDAFFGALVKAVDQVAQQHQKYVAIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNL
                     SGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGAGTPD
                     MPGGEAALVKLLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIAR
                     YTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAI
                     TNSTNQAM"
     protein_bind    1850..1871
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      2062..2606
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        3132..3234
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             3235..3891
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata
       61 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca
      121 gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag
      181 cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga
      241 ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa aaacggttag
      301 cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag
      361 atccggaaca taatggtgca gggcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
      421 gccgggggac tgttgggcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca
      481 gtcataagtg cggcgacgac accattcctt gcggcggcgg tgctcaacgg cctcaaccta
      541 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaga tcccggacac
      601 catcgaatgg cgcaaaacct ttcgcggtat ggtatgatag cgcccggaag agagtcaatt
      661 cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
      721 tcatcagacc gtttccaatg tggtgaacca ggccagccac gtttctgcga aaacgcggga
      781 aaaagtggaa gcggcgatgg cggagctcaa ttacattccc aaccgcgtgg cagcccaact
      841 ggcgggcaaa cagtcggaca ccataggcgt ggtggtgatg gacgttactg atgcgttttt
      901 cggcgcgctg gtaaaagcgg tggatcaggt cgctcagcag catcagaaat acgtggcaat
      961 cggcaatagc tatcatgaag cggaaaaaga gcgtcacgcc attgaggtgt taattcgcca
     1021 gcgttgtaat gcgttgattg ttcactcaaa agcattgagt gacgatgaac tggcgcaatt
     1081 tatggataac attcccggta tggtgttaat caaccgcgtt gtgccggggt acgcccatcg
     1141 ttgcgtttgt ctggataatc tcagcggtgc ccgaatggcg acgcgcatgt tgctgaataa
     1201 cggtcatcaa cgtattggtt atctttcttc cagccacggc attgaagatg acgccatgcg
     1261 taaagcaggc tggatgagtg cgttgaaaga gcaggatatt attccgccgg aaagctggat
     1321 tggcgctggt acgccggaca tgccgggcgg tgaggcggcg ttggttaaac tgctggggcg
     1381 caatctacaa cttaccgctg tatttgctta taacgacaat atggccgctg gcgcactgac
     1441 agcattaaaa gataatggca ttgcgattcc gttacatctc tcaatcatcg ggttcgatga
     1501 tattcccatc gcccgttaca ccgacccgca attaacgacc gtgcgttatc ccattgcttc
     1561 aatggcgaaa ttagccaccg aactggcctt gcagggggca gcaggcaata ttgatcctcg
     1621 tgccagccac tgttttatgc cgacgttagt gcgtcgccat tctgtcgcaa cacgccagaa
     1681 tgcggcggcg atcactaact caacaaatca ggcgatgtaa ctggtgaaaa gaaaaaccac
     1741 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     1801 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtaagtt
     1861 agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc aacccagtca
     1921 gctccttccg gtgggcgcgg ggcatgacta acatgagaat tacaacttat atcgtatggg
     1981 gctgacttca ggtgctacat ttgaagagat aaattgcact gaaatctaga aatattttat
     2041 ctgattaata agatgatctt cttgagatcg ttttggtctg cgcgtaatct cttgctctga
     2101 aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt ctctgagcta ccaactcttt
     2161 gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc
     2221 ttaaccggcg catgacttca agactaactc ctctaaatca attaccagtg gctgctgcca
     2281 gtggtgcttt tgcatgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc
     2341 agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc
     2401 cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg
     2461 gtaaaccgaa aggcaggaac aggagagcgc acgagggagc cgccagggga aacgcctggt
     2521 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct
     2581 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt
     2641 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg
     2701 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta
     2761 tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca
     2821 ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag cgctgatgtc
     2881 cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc tgaacaggag ggacagctga
     2941 tagaaacaga agccactgga gcacctcaaa aacaccatca tacactaaat cagtaagttg
     3001 gcagcatcac ccgacgcact ttgcgccgaa taaatacctg tgacggaaga tcacttcgca
     3061 gaataaataa atcctggtgt ccctgttgat accgggaagc cctgggccaa cttttggcga
     3121 aaatgagacg ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca
     3181 ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag
     3241 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     3301 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     3361 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     3421 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
     3481 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     3541 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     3601 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     3661 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     3721 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     3781 gtgctgatgc cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc
     3841 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaa
//
