LOCUS       40924_38703        4406 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT7A-actP-MCS-agsT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Chung SM, Frankman EL, Tzfira T.
  TITLE     A versatile vector system for multiple gene expression in plants
  JOURNAL   Trends Plant Sci. 10 (8), 357-361 (2005)
  PUBMED    15993643
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Chung S-M., Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2005) Department of Biochemistry and Cell Biology,
            State University of New York at Stony Brook, Stony Brook, NY 11794, 
            USA
REFERENCE   3  (bases 1 to 4406)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4406)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Trends 
            Plant Sci."; date: "2005"; volume: "10"; issue: "8"; pages: 
            "357-361"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-APR-2005) Department of Biochemistry and Cell Biology, State 
            University of New York at Stony Brook, Stony Brook, NY 11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      436..1652
                     /label=actin promoter
                     /note="actin promoter"
                     /regulatory_class="promoter"
     misc_feature    1653..1718
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1731..2136
                     /label=agropine synthase terminator
                     /note="agropine synthase terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2185..2201)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2209..2225)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2233..2263)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2278..2299)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2587..3175)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3349..4206)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4207..4311)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccca taaagccgtc agtgtccgca
      421 taaagaacca ccggtaacta tttttatgta tgcaagagtc agcatatgta taattgattc
      481 agaatcgttt tgacgagttc ggatgtagta gtagccatta tttaatgtac atactaatcg
      541 tgaatagtga atatgatgaa acattgtatc ttattgtata aatatccata aacacatcat
      601 gaaagacact ttctttcacg gtctgaatta attatgatac aattctaata gaaaacgaat
      661 taaattacgt tgaattgtat gaaatctaat tgaacaagcc aaccacgacg acgactaacg
      721 ttgcctggat tgactcggtt taagttaacc actaaaaaaa cggagctgtc atgtaacacg
      781 cggatcgagc aggtcacagt catgaagcca tcaaagcaaa agaactaatc caagggctga
      841 gatgattaat tagtttaaaa attagttaac acgagggaaa aggctgtctg acagccaggt
      901 cacgttatct ttacctgtgg tcgaaatgat tcgtgtctgt cgattttaat tatttttttg
      961 aaaggccgaa aataaagttg taagagataa acccgcctat ataaattcat atattttcct
     1021 ctccgctttg aattgtctcg ttgtcctcct cactttcatc agccgttttg aatctccggc
     1081 gacttgacag agaagaacaa ggaagaagac taagagagaa agtaagagat aatccaggag
     1141 attcattctc cgttttgaat cttcctcaat ctcatcttct tccgctcttt ctttccaagg
     1201 taataggaac tttctggatc tactttattt gctggatctc gatcttgctt tctcaatttc
     1261 gttgagatct ggaattcgtt taatttggat ctgtgaacct ccactaaatc ttttggtttt
     1321 actagaatcg atctaagttg accgatcagt tagctcgatt atagctacca gaatttggct
     1381 tgaccttgat ggagagatcc atgttcatgt tacctgggaa atgatttgta tatgtgaatt
     1441 gaaatctgaa ctgttgaagt tagattgaat ctgaacactg tcaatgttag attgaatctg
     1501 aacactgttt aaggttagat gaagtttgtg tatagattct tcgaaacttt aggatttgta
     1561 gtgtcgtacg ttgaacagaa agctatttct gattcaatca gggtttattt gactgtattg
     1621 aactcttttt gtgtgtttgc agctcataaa aatccggact cagatctcga gctcaagctt
     1681 cgaattctgc agtcgacggt accgcgggcc cgggatccac ctagtctaga gaattaacag
     1741 aggtggatgg acagacccgt tcttacaccg gactgggcgc gggataggat attcagattg
     1801 ggatgggatt gagcttaaag ccggcgctga gaccatgctc aaggtaggca atgtcctcag
     1861 cgtcgagccc ggcatctatg tcgagggcat tggtggagcg cgcttcgggg ataccgtgct
     1921 tgtaactgag accggatatg aggccctcac tccgcttgat cttggcaaag atatttgacg
     1981 catttattag tatgtgttaa ttttcatttg cagtgcagta ttttctattc gatctttatg
     2041 taattcgtta caattaataa atattcaaat cagattattg actgtcattt gtatcaaatc
     2101 gtgtttaatg gatattttta ttataatatt gatgatgcgg ccgccataaa gccgtcagtg
     2161 tccgcataaa gaaccgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     2221 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     2281 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     2341 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2401 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2461 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2521 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2581 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2641 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2701 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2761 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2821 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2881 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2941 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3001 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     3061 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3121 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3181 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3241 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3301 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3361 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3421 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3481 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3541 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3601 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3661 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3721 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3781 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3841 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3901 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3961 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     4021 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     4081 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     4141 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4201 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4261 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4321 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     4381 taggcgtatc acgaggccct ttcgtc
//
