LOCUS       40924_38693        4412 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT7-actP-MCS-agsT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Chung SM, Frankman EL, Tzfira T.
  TITLE     A versatile vector system for multiple gene expression in plants
  JOURNAL   Trends Plant Sci. 10 (8), 357-361 (2005)
  PUBMED    15993643
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Chung S-M., Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2005) Department of Biochemistry and Cell Biology,
            State University of New York at Stony Brook, Stony Brook, NY 11794, 
            USA
REFERENCE   3  (bases 1 to 4412)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4412)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Trends 
            Plant Sci."; date: "2005"; volume: "10"; issue: "8"; pages: 
            "357-361"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-APR-2005) Department of Biochemistry and Cell Biology, State 
            University of New York at Stony Brook, Stony Brook, NY 11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      436..1652
                     /label=actin promoter
                     /note="actin promoter"
                     /regulatory_class="promoter"
     misc_feature    1659..1724
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1737..2142
                     /label=agropine synthase terminator
                     /note="agropine synthase terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2191..2207)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2215..2231)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2239..2269)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2284..2305)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2593..3181)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3355..4212)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4213..4317)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccca taaagccgtc agtgtccgca
      421 taaagaacca ccggtaacta tttttatgta tgcaagagtc agcatatgta taattgattc
      481 agaatcgttt tgacgagttc ggatgtagta gtagccatta tttaatgtac atactaatcg
      541 tgaatagtga atatgatgaa acattgtatc ttattgtata aatatccata aacacatcat
      601 gaaagacact ttctttcacg gtctgaatta attatgatac aattctaata gaaaacgaat
      661 taaattacgt tgaattgtat gaaatctaat tgaacaagcc aaccacgacg acgactaacg
      721 ttgcctggat tgactcggtt taagttaacc actaaaaaaa cggagctgtc atgtaacacg
      781 cggatcgagc aggtcacagt catgaagcca tcaaagcaaa agaactaatc caagggctga
      841 gatgattaat tagtttaaaa attagttaac acgagggaaa aggctgtctg acagccaggt
      901 cacgttatct ttacctgtgg tcgaaatgat tcgtgtctgt cgattttaat tatttttttg
      961 aaaggccgaa aataaagttg taagagataa acccgcctat ataaattcat atattttcct
     1021 ctccgctttg aattgtctcg ttgtcctcct cactttcatc agccgttttg aatctccggc
     1081 gacttgacag agaagaacaa ggaagaagac taagagagaa agtaagagat aatccaggag
     1141 attcattctc cgttttgaat cttcctcaat ctcatcttct tccgctcttt ctttccaagg
     1201 taataggaac tttctggatc tactttattt gctggatctc gatcttgctt tctcaatttc
     1261 gttgagatct ggaattcgtt taatttggat ctgtgaacct ccactaaatc ttttggtttt
     1321 actagaatcg atctaagttg accgatcagt tagctcgatt atagctacca gaatttggct
     1381 tgaccttgat ggagagatcc atgttcatgt tacctgggaa atgatttgta tatgtgaatt
     1441 gaaatctgaa ctgttgaagt tagattgaat ctgaacactg tcaatgttag attgaatctg
     1501 aacactgttt aaggttagat gaagtttgtg tatagattct tcgaaacttt aggatttgta
     1561 gtgtcgtacg ttgaacagaa agctatttct gattcaatca gggtttattt gactgtattg
     1621 aactcttttt gtgtgtttgc agctcataaa aaccatggtc cggactcaga tctcgagctc
     1681 aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccacctag tctagagaat
     1741 taacagaggt ggatggacag acccgttctt acaccggact gggcgcggga taggatattc
     1801 agattgggat gggattgagc ttaaagccgg cgctgagacc atgctcaagg taggcaatgt
     1861 cctcagcgtc gagcccggca tctatgtcga gggcattggt ggagcgcgct tcggggatac
     1921 cgtgcttgta actgagaccg gatatgaggc cctcactccg cttgatcttg gcaaagatat
     1981 ttgacgcatt tattagtatg tgttaatttt catttgcagt gcagtatttt ctattcgatc
     2041 tttatgtaat tcgttacaat taataaatat tcaaatcaga ttattgactg tcatttgtat
     2101 caaatcgtgt ttaatggata tttttattat aatattgatg atgcggccgc cataaagccg
     2161 tcagtgtccg cataaagaac cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     2221 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     2281 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2341 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2401 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2461 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2521 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2581 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2641 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2701 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2761 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     2821 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2881 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2941 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3001 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     3061 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3121 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3181 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3241 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3301 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3361 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3421 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3481 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3541 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3601 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3661 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3721 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3781 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3841 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3901 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3961 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4021 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4081 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4141 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4201 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4261 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4321 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     4381 taaaaatagg cgtatcacga ggccctttcg tc
//
