LOCUS       40924_38683        4693 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT6A-rbcP-MCS-rbcT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Chung SM, Frankman EL, Tzfira T.
  TITLE     A versatile vector system for multiple gene expression in plants
  JOURNAL   Trends Plant Sci. 10 (8), 357-361 (2005)
  PUBMED    15993643
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Chung S-M., Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2005) Department of Biochemistry and Cell Biology,
            State University of New York at Stony Brook, Stony Brook, NY 11794, 
            USA
REFERENCE   3  (bases 1 to 4693)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4693)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Trends 
            Plant Sci."; date: "2005"; volume: "10"; issue: "8"; pages: 
            "357-361"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-APR-2005) Department of Biochemistry and Cell Biology, State 
            University of New York at Stony Brook, Stony Brook, NY 11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      435..1437
                     /note="RbcS1; rubisco small subunit promoter"
                     /regulatory_class="promoter"
     misc_feature    1438..1494
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1507..2424
                     /note="RbcS1; rubisco small subunit terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2472..2488)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2496..2512)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2520..2550)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2565..2586)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2874..3462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3636..4493)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4494..4598)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct
      421 gtttgccaac cggtcttaga caaacacccc ttgttataca aagaatttcg ctttacaaaa
      481 tcaaattcga gaaaataata tatgcactaa ataagatcat tcggatctaa tctaaccaat
      541 tacgatacgc tttgggtaca cttgattttt gtttcagtgg ttacatatat cttgttttat
      601 atgctatctt taaggatctg cacaaagatt atttgttgat gttcttgatg gggctcagaa
      661 gatttgatat gatacactct aatctttagg agataccagc caggattata ttcagtaaga
      721 caatcaaatt ttacgtgttc aaactcgtta tcttttcatt caaaggatga gccagaatct
      781 ttatagaatg attgcaatcg agaatatgtt cggccgatat gcctttgttg gcttcaatat
      841 tctacatatc acacaagaat cgaccgtatt gtaccctctt tccataaagg aaaacacaat
      901 atgcagatgc ttttttccca catgcagtaa catataggta ttcaaaaatg gctaaaagaa
      961 gttggataac aaattgacaa ctatttccat ttctgttata taaatttcac aacacacaaa
     1021 agcccgtaat caagagtctg cccatgtacg aaataacttc tattatttgg tattgggcct
     1081 aagcccagct cagagtacgt gggggtacca catataggaa ggtaacaaaa tactgcaaga
     1141 tagccccata acgtaccagc ctctccttac cacgaagaga taagatataa gacccaccct
     1201 gccacgtgtc acatcgtcat ggtggttaat gataagggat tacatccttc tatgtttgtg
     1261 gacatgatgc atgtaatgtc atgagccaca agatccaatg gccacaggaa cgtaagaatg
     1321 tagatagatt tgattttgtc cgttagatag caaacaacat tataaaaggt gtgtatcaat
     1381 aggaactaat tcactcattg gattcataga agtccattcc tcctaagtat ctagaaaaga
     1441 tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccacctag
     1501 tctagatcat aagcccgatg gctactaagt tttactattt accaagactt ttgaatatta
     1561 accttcttgt aacgagtcgg ttaaatttga ttgtttaggg ttttgtatta tttttttttg
     1621 gtcttttaat tcatcacttt aattccctaa ttgtctgttc atttcgttgt ttgtttccgg
     1681 atcgataatg aaatgtaaga gatatcatat ataaataata aattgtcgtt tcatatttgc
     1741 aatctttttt ttacaaacct ttaatcgttg tatgtatgac attttcttct tgttatatta
     1801 gggggaaata atgttaaata aaagtacaaa ataaactaca gtacatcgta ctgaataaat
     1861 tacctagcca aaaagtacac ctttccatat acttcctaca tgaaggcatt ttcaacattt
     1921 tcaaataagg aatgctacaa ccgcataata acatccacaa atttttttat aaaataacat
     1981 gtcagacagt gattgaaaga ttttattata gtttcgttat cttcttttct cattaagcga
     2041 atcactacct aacacgtcat tttgtgaaat attttttgaa tgtttttata tagttgtagc
     2101 attcctcttt tcaaattagg gtttgtttga gatagcattt cagccggttc atacaactta
     2161 aaagcatact ctaatgctgg aaaaaagact aaaaaatctt gtaagttagc gcagaatatt
     2221 gacccaaatt atatacacac atgaccccat atagagacta attacacttt taaccactaa
     2281 taattattac tgtattataa catctactaa ttaaacttgt gagtttttgc tagaattatt
     2341 atcatatata ctaaaaggca ggaacgcaaa cattgccccg gtactgtagc aactacggta
     2401 gacgcattaa ttgtctatag tggagcggcc gcacccataa tacccataat agctgtttgc
     2461 cagtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     2521 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     2581 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     2641 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2701 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2761 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2821 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2881 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2941 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3001 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3061 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3121 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3181 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3241 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3301 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3361 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3421 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3481 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3541 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3601 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3661 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3721 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3781 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3841 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3901 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3961 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     4021 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     4081 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4141 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     4201 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     4261 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4321 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4381 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4441 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4501 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4561 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4621 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     4681 aggccctttc gtc
//
