LOCUS       40924_38663        5947 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT6.supP.RNAi, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Dafny-Yelin M, Chung SM, Frankman EL, Tzfira T.
  TITLE     pSAT RNA interference vectors: a modular series for multiple gene 
            down-regulation in plants
  JOURNAL   Plant Physiol. 145 (4), 1272-1281 (2007)
  PUBMED    17766396
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Dafny-Yelin M, Chung S-M., Frankman EL, Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2007) Department of Molecular, Cellular and 
            Developmental Biology, The University of Michigan, Ann Arbor, MI 
            48109, USA
REFERENCE   3  (bases 1 to 5947)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5947)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1272-1281"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-JUL-2007) Department of Molecular, Cellular and Developmental 
            Biology, The University of Michigan, Ann Arbor, MI 48109, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1445..1827
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    1835..1875
                     /note="multiple cloning site I; MCS I"
     intron          1878..3226
                     /label=chsA intron
                     /note="chalcone synthase A intron from Petunia hybrida"
     misc_feature    3229..3272
                     /note="multiple cloning site II; MCS II"
     regulatory      3273..3678
                     /label=agropine synthase terminator
                     /note="agropine synthase terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(3726..3742)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3750..3766)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3774..3804)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3819..3840)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4128..4716)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4890..5747)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5748..5852)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct
      421 gtttgccaac cggggcgttg gccgattcat taatgcagct ggacatcatg ttggatatga
      481 aacaactatt atttatctac atgttttaga tgttatctga ttatttttat acgtagtctt
      541 ctattgatga ggagtctaag gctatagaat tatatatcta aatgattaat atatatatta
      601 ttaataatta acaataatta atatattata atttatatat atatatttta tattattata
      661 ataatattct tacaaatata attattatat tcgacggtat cgcgataagc tcgcggatcc
      721 ctgaaagcga cgttggatgt taacatctac aaattgcctt ttcttatcga ccatgtacgt
      781 aagcgcttac gtttttggtg gacccttgag gaaactggta gctgttgtgg gcctgtggtc
      841 tcaagatgga tcattaattt ccaccttcac ctacgatggg gggcatcgca ccggtgagta
      901 atattgtacg gctaagagcg aatttggcct gtaggatccc tgaaagcgac gttggatgtt
      961 aacatctaca aattgccttt tcttatcgac catgtacgta agcgcttacg tttttggtgg
     1021 acccttgagg aaactggtag ctgttgtggg cctgtggtct caagatggat cattaatttc
     1081 caccttcacc tacgatgggg ggcatcgcac cggtgagtaa tattgtacgg ctaagagcga
     1141 atttggcctg taggatccct gaaagcgacg ttggatgtta acatctacaa attgcctttt
     1201 cttatcgacc atgtacgtaa gcgcttacgt ttttggtgga cccttgagga aactggtagc
     1261 tgttgtgggc ctgtggtctc aagatggatc attaatttcc accttcacct acgatggggg
     1321 gcatcgcacc ggtgagtaat attgtacggc taagagcgaa tttggcctgt aggatccgcg
     1381 agctggtcaa tcccattgct tttgaagcag ctcaacattg atctctttct cgatcgaggg
     1441 agatttttca aatcagtgcg caagacgtga cgtaagtatc cgagtcagtt tttatttttc
     1501 tactaatttg gtcgtttatt tcggcgtgta ggacatggca accgggcctg aatttcgcgg
     1561 gtattctgtt tctattccaa ctttttcttg atccgcagcc attaacgact tttgaataga
     1621 tacgctgaca cgccaagcct cgctagtcaa aagtgtacca aacaacgctt tacagcaaga
     1681 acggaatgcg cgtgacgctc gcggtgacgc catttcgcct tttcagaaat ggataaatag
     1741 ccttgcttcc tattatatct tcccccaaat taccaataca ttacactagc atctgaattt
     1801 cataaccaat ctcgatacac caaatcgact ctagccatgg tccggactca gatctcgagc
     1861 tcaagcttcg aattcgtgta agaatttctt atgttacatt attacattca acgttttatc
     1921 ttaattggct cttcatttga ttgaaatttg acaattattt cttgtttttt tttttgtcac
     1981 actctttttg ggttggggtg gccgacgaat tgtgggaagg tagaaagagg ggaggacttt
     2041 tgttatactc cattagtaat tactgtttcc gtttcaattt atgtgacaat atttcctttt
     2101 tagtcggttc caaaagaaaa tgtcagcatt ataaacaatt taattttgaa attacaattt
     2161 tgccattaat aaaatgattt acaaccacaa aagtatctat gagcctgttt gggtgggctt
     2221 ataagcagct tattttaagt ggcttataag tcaaaaagtg acantttttg agaagttaga
     2281 aaatcctaac ttctcaaaaa gtagctttta agccacttat gacttataag tccaaaaatt
     2341 tttaagttac caaacatata ttaatgggtt tataagctta taagccactt ttaagctcac
     2401 ccaaacgggt tctatgtctc actttagact acaaatttta aaagtcttca tttatttctt
     2461 aatctccgtg gcgagtnaaa ctataacaca taaagtgaaa cggagggaat aagatggagt
     2521 cataaactaa tccaaatcta tactctctcc gttaatttgt tttttagttt gatttggtac
     2581 attaataaaa cagatttttc gaaggttata aacacagaca gatgtttccc agcgagctag
     2641 caaaattcca agatttctgt cgaaaattcg tgtgtttcta gctagtactt gatgttatct
     2701 ttaacctttt agtaattttt tgtccttttc tttctatttt tcatcttaca atgaattatg
     2761 agcaagttcc ttaagtagca tcacacgtga gatgtttttt atgatattga ctaaatccaa
     2821 tctttaccat tccttaacta gtaaaataca acacatgtta attgatacat tgcttaacac
     2881 tgaggttaga aaattttaga aattagttgt ccaaatgctt tgaaattaga aatctttaat
     2941 cccttatttt tttttaaaat gttttttctc actccaaaga aagagaaact gacatgaaag
     3001 ctcaaaagat catgaatctt actaactttg tggaactaaa tgtacatcag aatgtttctg
     3061 acatgtgaaa atgaaagctc ttaattttct tcttttattt attgagggtt tttgcatgct
     3121 atgcattcaa tttgagtact ttaaagcacc tataaacact tacttacact tgccttggag
     3181 tttatgtttt agtgttttct tcacatcttt tttggtcaat ttgcaggtct gcagtcgacg
     3241 gtaccgcggg cccgggatcc acctagtcta gagaattaac agaggtggat ggacagaccc
     3301 gttcttacac cggactgggc gcgggatagg atattcagat tgggatggga ttgagcttaa
     3361 agccggcgct gagaccatgc tcaaggtagg caatgtcctc agcgtcgagc ccggcatcta
     3421 tgtcgagggc attggtggag cgcgcttcgg ggataccgtg cttgtaactg agaccggata
     3481 tgaggccctc actccgcttg atcttggcaa agatatttga cgcatttatt agtatgtgtt
     3541 aattttcatt tgcagtgcag tattttctat tcgatcttta tgtaattcgt tacaattaat
     3601 aaatattcaa atcagattat tgactgtcat ttgtatcaaa tcgtgtttaa tggatatttt
     3661 tattataata ttgatgatgc ggccgcaccc ataataccca taatagctgt ttgccagtaa
     3721 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     3781 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3841 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     3901 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     3961 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     4021 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     4081 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     4141 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     4201 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     4261 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     4321 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     4381 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4441 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4501 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4561 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4621 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4681 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4741 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4801 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4861 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4921 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4981 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     5041 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     5101 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     5161 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     5221 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     5281 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     5341 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     5401 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     5461 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     5521 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     5581 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     5641 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     5701 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     5761 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5821 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     5881 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     5941 tttcgtc
//
