LOCUS       40924_38658        6062 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT6.rbcP.RNAi, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Dafny-Yelin M, Chung SM, Frankman EL, Tzfira T.
  TITLE     pSAT RNA interference vectors: a modular series for multiple gene 
            down-regulation in plants
  JOURNAL   Plant Physiol. 145 (4), 1272-1281 (2007)
  PUBMED    17766396
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Dafny-Yelin M, Chung S-M., Frankman EL, Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2007) Department of Molecular, Cellular and 
            Developmental Biology, The University of Michigan, Ann Arbor, MI 
            48109, USA
REFERENCE   3  (bases 1 to 6062)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6062)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1272-1281"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-JUL-2007) Department of Molecular, Cellular and Developmental 
            Biology, The University of Michigan, Ann Arbor, MI 48109, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      435..1437
                     /note="ribulose-1,5-bisphosphate carboxylase/oxygenase
                     (rubisco) small subunit promoter"
                     /regulatory_class="promoter"
     misc_feature    1438..1478
                     /note="multiple cloning site I; MCS I"
     intron          1481..2829
                     /label=chsA intron
                     /note="chalcone synthase A intron from Petunia hybrida"
     misc_feature    2832..2875
                     /note="multiple cloning site II; MCS II"
     regulatory      2876..3793
                     /note="ribulose-1,5-bisphosphate carboxylase/oxygenase
                     (rubisco) small subunit terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(3841..3857)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3865..3881)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3889..3919)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3934..3955)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4243..4831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5005..5862)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5863..5967)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct
      421 gtttgccaac cggtcttaga caaacacccc ttgttataca aagaatttcg ctttacaaaa
      481 tcaaattcga gaaaataata tatgcactaa ataagatcat tcggatctaa tctaaccaat
      541 tacgatacgc tttgggtaca cttgattttt gtttcagtgg ttacatatat cttgttttat
      601 atgctatctt taaggatctg cacaaagatt atttgttgat gttcttgatg gggctcagaa
      661 gatttgatat gatacactct aatctttagg agataccagc caggattata ttcagtaaga
      721 caatcaaatt ttacgtgttc aaactcgtta tcttttcatt caaaggatga gccagaatct
      781 ttatagaatg attgcaatcg agaatatgtt cggccgatat gcctttgttg gcttcaatat
      841 tctacatatc acacaagaat cgaccgtatt gtaccctctt tccataaagg aaaacacaat
      901 atgcagatgc ttttttccca catgcagtaa catataggta ttcaaaaatg gctaaaagaa
      961 gttggataac aaattgacaa ctatttccat ttctgttata taaatttcac aacacacaaa
     1021 agcccgtaat caagagtctg cccatgtacg aaataacttc tattatttgg tattgggcct
     1081 aagcccagct cagagtacgt gggggtacca catataggaa ggtaacaaaa tactgcaaga
     1141 tagccccata acgtaccagc ctctccttac cacgaagaga taagatataa gacccaccct
     1201 gccacgtgtc acatcgtcat ggtggttaat gataagggat tacatccttc tatgtttgtg
     1261 gacatgatgc atgtaatgtc atgagccaca agatccaatg gccacaggaa cgtaagaatg
     1321 tagatagatt tgattttgtc cgttagatag caaacaacat tataaaaggt gtgtatcaat
     1381 aggaactaat tcactcattg gattcataga agtccattcc tcctaagtat ctagaaacca
     1441 tggtccggac tcagatctcg agctcaagct tcgaattcgt gtaagaattt cttatgttac
     1501 attattacat tcaacgtttt atcttaattg gctcttcatt tgattgaaat ttgacaatta
     1561 tttcttgttt ttttttttgt cacactcttt ttgggttggg gtggccgacg aattgtggga
     1621 aggtagaaag aggggaggac ttttgttata ctccattagt aattactgtt tccgtttcaa
     1681 tttatgtgac aatatttcct ttttagtcgg ttccaaaaga aaatgtcagc attataaaca
     1741 atttaatttt gaaattacaa ttttgccatt aataaaatga tttacaacca caaaagtatc
     1801 tatgagcctg tttgggtggg cttataagca gcttatttta agtggcttat aagtcaaaaa
     1861 gtgacanttt ttgagaagtt agaaaatcct aacttctcaa aaagtagctt ttaagccact
     1921 tatgacttat aagtccaaaa atttttaagt taccaaacat atattaatgg gtttataagc
     1981 ttataagcca cttttaagct cacccaaacg ggttctatgt ctcactttag actacaaatt
     2041 ttaaaagtct tcatttattt cttaatctcc gtggcgagtn aaactataac acataaagtg
     2101 aaacggaggg aataagatgg agtcataaac taatccaaat ctatactctc tccgttaatt
     2161 tgttttttag tttgatttgg tacattaata aaacagattt ttcgaaggtt ataaacacag
     2221 acagatgttt cccagcgagc tagcaaaatt ccaagatttc tgtcgaaaat tcgtgtgttt
     2281 ctagctagta cttgatgtta tctttaacct tttagtaatt ttttgtcctt ttctttctat
     2341 ttttcatctt acaatgaatt atgagcaagt tccttaagta gcatcacacg tgagatgttt
     2401 tttatgatat tgactaaatc caatctttac cattccttaa ctagtaaaat acaacacatg
     2461 ttaattgata cattgcttaa cactgaggtt agaaaatttt agaaattagt tgtccaaatg
     2521 ctttgaaatt agaaatcttt aatcccttat ttttttttaa aatgtttttt ctcactccaa
     2581 agaaagagaa actgacatga aagctcaaaa gatcatgaat cttactaact ttgtggaact
     2641 aaatgtacat cagaatgttt ctgacatgtg aaaatgaaag ctcttaattt tcttctttta
     2701 tttattgagg gtttttgcat gctatgcatt caatttgagt actttaaagc acctataaac
     2761 acttacttac acttgccttg gagtttatgt tttagtgttt tcttcacatc ttttttggtc
     2821 aatttgcagg tctgcagtcg acggtaccgc gggcccggga tccacctagt ctagatcata
     2881 agcccgatgg ctactaagtt ttactattta ccaagacttt tgaatattaa ccttcttgta
     2941 acgagtcggt taaatttgat tgtttagggt tttgtattat ttttttttgg tcttttaatt
     3001 catcacttta attccctaat tgtctgttca tttcgttgtt tgtttccgga tcgataatga
     3061 aatgtaagag atatcatata taaataataa attgtcgttt catatttgca atcttttttt
     3121 tacaaacctt taatcgttgt atgtatgaca ttttcttctt gttatattag ggggaaataa
     3181 tgttaaataa aagtacaaaa taaactacag tacatcgtac tgaataaatt acctagccaa
     3241 aaagtacacc tttccatata cttcctacat gaaggcattt tcaacatttt caaataagga
     3301 atgctacaac cgcataataa catccacaaa tttttttata aaataacatg tcagacagtg
     3361 attgaaagat tttattatag tttcgttatc ttcttttctc attaagcgaa tcactaccta
     3421 acacgtcatt ttgtgaaata ttttttgaat gtttttatat agttgtagca ttcctctttt
     3481 caaattaggg tttgtttgag atagcatttc agccggttca tacaacttaa aagcatactc
     3541 taatgctgga aaaaagacta aaaaatcttg taagttagcg cagaatattg acccaaatta
     3601 tatacacaca tgaccccata tagagactaa ttacactttt aaccactaat aattattact
     3661 gtattataac atctactaat taaacttgtg agtttttgct agaattatta tcatatatac
     3721 taaaaggcag gaacgcaaac attgccccgg tactgtagca actacggtag acgcattaat
     3781 tgtctatagt ggagcggccg cacccataat acccataata gctgtttgcc agtaatcatg
     3841 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     3901 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     3961 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     4021 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4081 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4141 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4201 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4261 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4321 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4381 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     4441 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4501 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4561 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4621 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4681 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4741 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4801 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4861 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4921 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4981 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5041 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5101 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5161 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5221 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5281 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5341 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5401 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5461 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5521 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5581 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5641 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5701 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5761 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5821 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5881 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5941 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     6001 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     6061 tc
//
