LOCUS       40924_38648        5238 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT6.35S.RNAi, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Dafny-Yelin M, Chung SM, Frankman EL, Tzfira T.
  TITLE     pSAT RNA interference vectors: a modular series for multiple gene 
            down-regulation in plants
  JOURNAL   Plant Physiol. 145 (4), 1272-1281 (2007)
  PUBMED    17766396
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Dafny-Yelin M, Chung S-M., Frankman EL, Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2007) Department of Molecular, Cellular and 
            Developmental Biology, The University of Michigan, Ann Arbor, MI 
            48109, USA
REFERENCE   3  (bases 1 to 5238)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5238)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1272-1281"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-JUL-2007) Department of Molecular, Cellular and Developmental 
            Biology, The University of Michigan, Ann Arbor, MI 48109, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        509..1180
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     5'UTR           1190..1320
                     /note="translational enhancer; 5' UTR from tobacco etch
                     virus"
     misc_feature    1321..1361
                     /note="multiple cloning site I; MCS I"
     intron          1364..2712
                     /label=chsA intron
                     /note="chalcone synthase A intron from Petunia hybrida"
     misc_feature    2715..2758
                     /note="multiple cloning site II; MCS II"
     regulatory      2759..2969
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(3017..3033)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3041..3057)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3065..3095)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3110..3131)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3419..4007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4181..5038)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5039..5143)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct
      421 gtttgccaac cggtcaacat gtggagcacg acacacttgt ctactccaaa aatatcaaag
      481 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa
      541 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg
      601 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct
      661 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
      721 acgttccaac cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca
      781 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
      841 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
      901 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
      961 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     1021 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     1081 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
     1141 cttcctctat ataaggaagt tcatttcatt tggagaggac gtcgagagtt ctcaacacaa
     1201 catatacaaa acaaacgaat ctcaagcaat caagcattct acttctattg cagcaattta
     1261 aatcatttct tttaaagcaa aagcaatttt ctgaaaattt tcaccattta cgaacgatag
     1321 ccatggtccg gactcagatc tcgagctcaa gcttcgaatt cgtgtaagaa tttcttatgt
     1381 tacattatta cattcaacgt tttatcttaa ttggctcttc atttgattga aatttgacaa
     1441 ttatttcttg tttttttttt tgtcacactc tttttgggtt ggggtggccg acgaattgtg
     1501 ggaaggtaga aagaggggag gacttttgtt atactccatt agtaattact gtttccgttt
     1561 caatttatgt gacaatattt cctttttagt cggttccaaa agaaaatgtc agcattataa
     1621 acaatttaat tttgaaatta caattttgcc attaataaaa tgatttacaa ccacaaaagt
     1681 atctatgagc ctgtttgggt gggcttataa gcagcttatt ttaagtggct tataagtcaa
     1741 aaagtgacac tttttgagaa gttagaaaat cctaacttct caaaaagtag cttttaagcc
     1801 acttatgact tataagtcca aaaattttta agttaccaaa catatattaa tgggtttata
     1861 agcttataag ccacttttaa gctcacccaa acgggttcta tgtctcactt tagactacaa
     1921 attttaaaag tcttcattta tttcttaatc tccgtggcga gtcaaactat aacacataaa
     1981 gtgaaacgga gggaataaga tggagtcata aactaatcca aatctatact ctctccgtta
     2041 atttgttttt tagtttgatt tggtacatta ataaaacaga tttttcgaag gttataaaca
     2101 cagacagatg tttcccagcg agctagcaaa attccaagat ttctgtcgaa aattcgtgtg
     2161 tttctagcta gtacttgatg ttatctttaa ccttttagta attttttgtc cttttctttc
     2221 tatttttcat cttacaatga attatgagca agttccttaa gtagcatcac acgtgagatg
     2281 ttttttatga tattgactaa atccaatctt taccattcct taactagtaa aatacaacac
     2341 atgttaattg atacattgct taacactgag gttagaaaat tttagaaatt agttgtccaa
     2401 atgctttgaa attagaaatc tttaatccct tatttttttt taaaatgttt tttctcactc
     2461 caaagaaaga gaaactgaca tgaaagctca aaagatcatg aatcttacta actttgtgga
     2521 actaaatgta catcagaatg tttctgacat gtgaaaatga aagctcttaa ttttcttctt
     2581 ttatttattg agggtttttg catgctatgc attcaatttg agtactttaa agcacctata
     2641 aacacttact tacacttgcc ttggagttta tgttttagtg ttttcttcac atcttttttg
     2701 gtcaatttgc aggtctgcag tcgacggtac cgcgggcccg ggatccacct agtctagagt
     2761 ccgcaaaaat caccagtctc tctctacaaa tctatctctc tctatttttc tccagaataa
     2821 tgtgtgagta gttcccagat aagggaatta gggttcttat agggtttcgc tcatgtgttg
     2881 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
     2941 tctaattcct aaaaccaaaa tccagtgacg cggccgcacc cataataccc ataatagctg
     3001 tttgccagta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     3061 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     3121 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     3181 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     3241 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     3301 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     3361 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3421 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3481 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3541 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3601 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3661 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3721 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3781 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3841 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3901 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3961 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4021 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4081 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     4141 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     4201 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     4261 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     4321 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     4381 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     4441 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     4501 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     4561 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     4621 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     4681 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     4741 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     4801 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     4861 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4921 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4981 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     5041 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     5101 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     5161 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     5221 tcacgaggcc ctttcgtc
//
