LOCUS       40924_38633        4708 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT6-rbcP-MCS-rbcT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Chung SM, Frankman EL, Tzfira T.
  TITLE     A versatile vector system for multiple gene expression in plants
  JOURNAL   Trends Plant Sci. 10 (8), 357-361 (2005)
  PUBMED    15993643
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Chung S-M., Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2005) Department of Biochemistry and Cell Biology,
            State University of New York at Stony Brook, Stony Brook, NY 11794, 
            USA
REFERENCE   3  (bases 1 to 4708)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4708)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Trends 
            Plant Sci."; date: "2005"; volume: "10"; issue: "8"; pages: 
            "357-361"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-APR-2005) Department of Biochemistry and Cell Biology, State 
            University of New York at Stony Brook, Stony Brook, NY 11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      435..1437
                     /note="RbcS1; rubisco small subunit promoter"
                     /regulatory_class="promoter"
     misc_feature    1444..1509
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1522..2439
                     /note="RbcS1; rubisco small subunit terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2487..2503)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2511..2527)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2535..2565)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2580..2601)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2889..3477)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3651..4508)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4509..4613)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct
      421 gtttgccaac cggtcttaga caaacacccc ttgttataca aagaatttcg ctttacaaaa
      481 tcaaattcga gaaaataata tatgcactaa ataagatcat tcggatctaa tctaaccaat
      541 tacgatacgc tttgggtaca cttgattttt gtttcagtgg ttacatatat cttgttttat
      601 atgctatctt taaggatctg cacaaagatt atttgttgat gttcttgatg gggctcagaa
      661 gatttgatat gatacactct aatctttagg agataccagc caggattata ttcagtaaga
      721 caatcaaatt ttacgtgttc aaactcgtta tcttttcatt caaaggatga gccagaatct
      781 ttatagaatg attgcaatcg agaatatgtt cggccgatat gcctttgttg gcttcaatat
      841 tctacatatc acacaagaat cgaccgtatt gtaccctctt tccataaagg aaaacacaat
      901 atgcagatgc ttttttccca catgcagtaa catataggta ttcaaaaatg gctaaaagaa
      961 gttggataac aaattgacaa ctatttccat ttctgttata taaatttcac aacacacaaa
     1021 agcccgtaat caagagtctg cccatgtacg aaataacttc tattatttgg tattgggcct
     1081 aagcccagct cagagtacgt gggggtacca catataggaa ggtaacaaaa tactgcaaga
     1141 tagccccata acgtaccagc ctctccttac cacgaagaga taagatataa gacccaccct
     1201 gccacgtgtc acatcgtcat ggtggttaat gataagggat tacatccttc tatgtttgtg
     1261 gacatgatgc atgtaatgtc atgagccaca agatccaatg gccacaggaa cgtaagaatg
     1321 tagatagatt tgattttgtc cgttagatag caaacaacat tataaaaggt gtgtatcaat
     1381 aggaactaat tcactcattg gattcataga agtccattcc tcctaagtat ctagaaacca
     1441 tggtccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
     1501 ccgggatcca cctagtctag atcataagcc cgatggctac taagttttac tatttaccaa
     1561 gacttttgaa tattaacctt cttgtaacga gtcggttaaa tttgattgtt tagggttttg
     1621 tattattttt ttttggtctt ttaattcatc actttaattc cctaattgtc tgttcatttc
     1681 gttgtttgtt tccggatcga taatgaaatg taagagatat catatataaa taataaattg
     1741 tcgtttcata tttgcaatct tttttttaca aacctttaat cgttgtatgt atgacatttt
     1801 cttcttgtta tattaggggg aaataatgtt aaataaaagt acaaaataaa ctacagtaca
     1861 tcgtactgaa taaattacct agccaaaaag tacacctttc catatacttc ctacatgaag
     1921 gcattttcaa cattttcaaa taaggaatgc tacaaccgca taataacatc cacaaatttt
     1981 tttataaaat aacatgtcag acagtgattg aaagatttta ttatagtttc gttatcttct
     2041 tttctcatta agcgaatcac tacctaacac gtcattttgt gaaatatttt ttgaatgttt
     2101 ttatatagtt gtagcattcc tcttttcaaa ttagggtttg tttgagatag catttcagcc
     2161 ggttcataca acttaaaagc atactctaat gctggaaaaa agactaaaaa atcttgtaag
     2221 ttagcgcaga atattgaccc aaattatata cacacatgac cccatataga gactaattac
     2281 acttttaacc actaataatt attactgtat tataacatct actaattaaa cttgtgagtt
     2341 tttgctagaa ttattatcat atatactaaa aggcaggaac gcaaacattg ccccggtact
     2401 gtagcaacta cggtagacgc attaattgtc tatagtggag cggccgcacc cataataccc
     2461 ataatagctg tttgccagta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     2521 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     2581 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     2641 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     2701 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2761 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2821 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2881 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2941 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3001 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3061 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     3121 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3181 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3241 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3301 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     3361 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3421 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3481 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3541 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3601 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3661 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3721 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3781 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3841 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3901 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3961 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4021 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4081 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4141 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4201 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4261 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4321 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4381 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4441 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4501 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4561 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4621 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     4681 aataggcgta tcacgaggcc ctttcgtc
//
