LOCUS       40924_38628        3884 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT6-MCS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Tzfira T, Tian GW, Lacroix B, Vyas S, Li J, Leitner-Dagan Y, 
            Krichevsky A, Taylor T, Vainstein A, Citovsky V.
  TITLE     pSAT vectors: a modular series of plasmids for autofluorescent 
            protein tagging and expression of multiple genes in plants
  JOURNAL   Plant Mol. Biol. 57 (4), 503-516 (2005)
  PUBMED    15821977
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Tzfira T, Lacroix B, Citovsky V.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2004) Department of Biochemistry and Cell Biology,
            State University of New York Stony Brook, 100 Nicolls Rd, Stony 
            Brook, NY 11794, USA
REFERENCE   3  (bases 1 to 3884)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3884)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2005"; volume: "57"; issue: "4"; pages: "503-516"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-NOV-2004) Department of Biochemistry and Cell Biology, State 
            University of New York Stony Brook, 100 Nicolls Rd, Stony Brook, NY 
            11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        509..1180
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1190..1320
                     /note="5' UTR from tobacco etch virus; translational
                     enhancer"
     misc_feature    1327..1392
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1405..1615
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(1663..1679)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1687..1703)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1711..1741)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1756..1777)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2065..2653)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2827..3684)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3685..3789)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct
      421 gtttgccaac cggtcaacat gtggagcacg acacacttgt ctactccaaa aatatcaaag
      481 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa
      541 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg
      601 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct
      661 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
      721 acgttccaac cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca
      781 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
      841 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
      901 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
      961 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     1021 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     1081 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
     1141 cttcctctat ataaggaagt tcatttcatt tggagaggac gtcgagagtt ctcaacacaa
     1201 catatacaaa acaaacgaat ctcaagcaat caagcattct acttctattg cagcaattta
     1261 aatcatttct tttaaagcaa aagcaatttt ctgaaaattt tcaccattta cgaacgatag
     1321 ccatggtccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg
     1381 ggcccgggat ccacctagtc tagagtccgc aaaaatcacc agtctctctc tacaaatcta
     1441 tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg gaattagggt
     1501 tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt
     1561 tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtgacgcggc
     1621 cgcacccata atacccataa tagctgtttg ccagtaatca tggtcatagc tgtttcctgt
     1681 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     1741 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     1801 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     1861 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1921 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1981 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2041 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2101 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2161 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     2221 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     2281 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     2341 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     2401 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     2461 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     2521 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     2581 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     2641 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2701 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     2761 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2821 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     2881 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2941 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3001 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3061 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3121 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3181 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     3241 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     3301 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     3361 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     3421 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     3481 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     3541 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     3601 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     3661 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     3721 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     3781 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     3841 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
