LOCUS       40924_38578        4570 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT6-DsRed2-N1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Tzfira T, Tian GW, Lacroix B, Vyas S, Li J, Leitner-Dagan Y, 
            Krichevsky A, Taylor T, Vainstein A, Citovsky V.
  TITLE     pSAT vectors: a modular series of plasmids for autofluorescent 
            protein tagging and expression of multiple genes in plants
  JOURNAL   Plant Mol. Biol. 57 (4), 503-516 (2005)
  PUBMED    15821977
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Tzfira T, Lacroix B, Citovsky V.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2004) Department of Biochemistry and Cell Biology,
            State University of New York Stony Brook, 100 Nicolls Rd, Stony 
            Brook, NY 11794, USA
REFERENCE   3  (bases 1 to 4570)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4570)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2005"; volume: "57"; issue: "4"; pages: "503-516"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-NOV-2004) Department of Biochemistry and Cell Biology, State 
            University of New York Stony Brook, 100 Nicolls Rd, Stony Brook, NY 
            11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        509..1180
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1190..1320
                     /note="5' UTR from tobacco etch virus; translational
                     enhancer"
     misc_feature    1327..1392
                     /label=MCS
                     /note="multiple cloning site"
     CDS             1395..2069
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     regulatory      2091..2301
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2349..2365)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2373..2389)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2397..2427)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2442..2463)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2751..3339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3513..4370)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4371..4475)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct
      421 gtttgccaac cggtcaacat gtggagcacg acacacttgt ctactccaaa aatatcaaag
      481 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa
      541 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg
      601 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct
      661 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
      721 acgttccaac cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca
      781 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
      841 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
      901 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
      961 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     1021 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     1081 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
     1141 cttcctctat ataaggaagt tcatttcatt tggagaggac gtcgagagtt ctcaacacaa
     1201 catatacaaa acaaacgaat ctcaagcaat caagcattct acttctattg cagcaattta
     1261 aatcatttct tttaaagcaa aagcaatttt ctgaaaattt tcaccattta cgaacgatag
     1321 ccatggtccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg
     1381 ggcccgggat cctgatggcc tcctccgaga acgtcatcac cgagttcatg cgcttcaagg
     1441 tgcgcatgga gggcaccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     1501 gcccctacga gggccacaac accgtgaagc tgaaggtgac caagggcggc cccctgccct
     1561 tcgcctggga catcctgtcc ccccagttcc agtacggctc caaggtgtac gtgaagcacc
     1621 ccgccgacat ccccgactac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
     1681 tgatgaactt cgaggacggc ggcgtggcga ccgtgaccca ggactcctcc ctgcaggacg
     1741 gctgcttcat ctacaaggtg aagttcatcg gcgtgaactt cccctccgac ggccccgtga
     1801 tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg
     1861 tgctgaaggg cgagacccac aaggccctga agctgaagga cggcggccac tacctggtgg
     1921 agttcaagtc catctacatg gccaagaagc ccgtgcagct gcccggctac tactacgtgg
     1981 acgccaagct ggacatcacc tcccacaacg aggactacac catcgtggag cagtacgagc
     2041 gcaccgaggg ccgccaccac ctgttcctgt gaagatccac ctagtctaga gtccgcaaaa
     2101 atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag
     2161 tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata
     2221 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
     2281 ctaaaaccaa aatccagtga cgcggccgca cccataatac ccataatagc tgtttgccag
     2341 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     2401 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     2461 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     2521 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     2581 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2641 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2701 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2761 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2821 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2881 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2941 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3001 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3061 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3121 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3181 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3241 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3301 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3361 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3421 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3481 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3541 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3601 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3661 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3721 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3781 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3841 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3901 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     3961 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4021 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4081 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4141 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4201 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4261 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4321 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4381 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4441 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4501 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     4561 ccctttcgtc
//
