LOCUS       40924_38573        4546 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT6-DsRed2-C1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Tzfira T, Tian GW, Lacroix B, Vyas S, Li J, Leitner-Dagan Y, 
            Krichevsky A, Taylor T, Vainstein A, Citovsky V.
  TITLE     pSAT vectors: a modular series of plasmids for autofluorescent 
            protein tagging and expression of multiple genes in plants
  JOURNAL   Plant Mol. Biol. 57 (4), 503-516 (2005)
  PUBMED    15821977
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Tzfira T, Lacroix B, Citovsky V.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2004) Department of Biochemistry and Cell Biology,
            State University of New York Stony Brook, 100 Nicolls Rd, Stony 
            Brook, NY 11794, USA
REFERENCE   3  (bases 1 to 4546)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4546)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2005"; volume: "57"; issue: "4"; pages: "503-516"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-NOV-2004) Department of Biochemistry and Cell Biology, State 
            University of New York Stony Brook, 100 Nicolls Rd, Stony Brook, NY 
            11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        509..1180
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1190..1320
                     /note="5' UTR from tobacco etch virus; translational
                     enhancer"
     CDS             1323..1997
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     misc_feature    1998..2054
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      2067..2277
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2325..2341)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2349..2365)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2373..2403)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2418..2439)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2727..3315)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3489..4346)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4347..4451)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccac ccataatacc cataatagct
      421 gtttgccaac cggtcaacat gtggagcacg acacacttgt ctactccaaa aatatcaaag
      481 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa
      541 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg
      601 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct
      661 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
      721 acgttccaac cacgtcttca aagcaagtgg attgatgtga taacatggtg gagcacgaca
      781 cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga
      841 cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc
      901 actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata
      961 aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     1021 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     1081 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc
     1141 cttcctctat ataaggaagt tcatttcatt tggagaggac gtcgagagtt ctcaacacaa
     1201 catatacaaa acaaacgaat ctcaagcaat caagcattct acttctattg cagcaattta
     1261 aatcatttct tttaaagcaa aagcaatttt ctgaaaattt tcaccattta cgaacgatag
     1321 ccatggcctc ctccgagaac gtcatcaccg agttcatgcg cttcaaggtg cgcatggagg
     1381 gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg
     1441 gccacaacac cgtgaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca
     1501 tcctgtcccc ccagttccag tacggctcca aggtgtacgt gaagcacccc gccgacatcc
     1561 ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg
     1621 aggacggcgg cgtggcgacc gtgacccagg actcctccct gcaggacggc tgcttcatct
     1681 acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg ccccgtgatg cagaagaaga
     1741 ccatgggctg ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg
     1801 agacccacaa ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagtcca
     1861 tctacatggc caagaagccc gtgcagctgc ccggctacta ctacgtggac gccaagctgg
     1921 acatcacctc ccacaacgag gactacacca tcgtggagca gtacgagcgc accgagggcc
     1981 gccaccacct gttcctgaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg
     2041 cgggcccggg atccacctag tctagagtcc gcaaaaatca ccagtctctc tctacaaatc
     2101 tatctctctc tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg
     2161 gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta
     2221 tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgacgcg
     2281 gccgcaccca taatacccat aatagctgtt tgccagtaat catggtcata gctgtttcct
     2341 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     2401 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     2461 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     2521 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2581 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2641 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2701 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2761 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2821 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2881 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     2941 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3001 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3061 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3121 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     3181 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3241 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3301 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3361 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3421 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3481 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3541 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3601 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     3661 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3721 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3781 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3841 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3901 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3961 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4021 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4081 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4141 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4201 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4261 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4321 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4381 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4441 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     4501 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
