LOCUS       40924_38558        4678 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT5.nosP.RNAi, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Dafny-Yelin M, Chung SM, Frankman EL, Tzfira T.
  TITLE     pSAT RNA interference vectors: a modular series for multiple gene 
            down-regulation in plants
  JOURNAL   Plant Physiol. 145 (4), 1272-1281 (2007)
  PUBMED    17766396
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Dafny-Yelin M, Chung S-M., Frankman EL, Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2007) Department of Molecular, Cellular and 
            Developmental Biology, The University of Michigan, Ann Arbor, MI 
            48109, USA
REFERENCE   3  (bases 1 to 4678)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4678)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1272-1281"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-JUL-2007) Department of Molecular, Cellular and Developmental 
            Biology, The University of Michigan, Ann Arbor, MI 48109, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        514..693
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    716..756
                     /note="multiple cloning site I; MCS I"
     intron          759..2107
                     /label=chsA intron
                     /note="chalcone synthase A intron from Petunia hybrida"
     misc_feature    2110..2153
                     /note="multiple cloning site II; MCS II"
     terminator      2158..2410
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2457..2473)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2481..2497)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2505..2535)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2550..2571)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2859..3447)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3621..4478)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4479..4583)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcctc gctaccttag gaccgttata
      421 gttaaccggt gatcatgagc ggagaattaa gggagtcacg ttatgacccc cgccgatgac
      481 gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag gagccactca
      541 gccgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat tattgcgcgt
      601 tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa atgctccact
      661 gacgttccat aaattcccct cggtatccaa ttagagtctc atattcactc tcaatccatg
      721 gtccggactc agatctcgag ctcaagcttc gaattcgtgt aagaatttct tatgttacat
      781 tattacattc aacgttttat cttaattggc tcttcatttg attgaaattt gacaattatt
      841 tcttgttttt ttttttgtca cactcttttt gggttggggt ggccgacgaa ttgtgggaag
      901 gtagaaagag gggaggactt ttgttatact ccattagtaa ttactgtttc cgtttcaatt
      961 tatgtgacaa tatttccttt ttagtcggtt ccaaaagaaa atgtcagcat tataaacaat
     1021 ttaattttga aattacaatt ttgccattaa taaaatgatt tacaaccaca aaagtatcta
     1081 tgagcctgtt tgggtgggct tataagcagc ttattttaag tggcttataa gtcaaaaagt
     1141 gacanttttt gagaagttag aaaatcctaa cttctcaaaa agtagctttt aagccactta
     1201 tgacttataa gtccaaaaat ttttaagtta ccaaacatat attaatgggt ttataagctt
     1261 ataagccact tttaagctca cccaaacggg ttctatgtct cactttagac tacaaatttt
     1321 aaaagtcttc atttatttct taatctccgt ggcgagtnaa actataacac ataaagtgaa
     1381 acggagggaa taagatggag tcataaacta atccaaatct atactctctc cgttaatttg
     1441 ttttttagtt tgatttggta cattaataaa acagattttt cgaaggttat aaacacagac
     1501 agatgtttcc cagcgagcta gcaaaattcc aagatttctg tcgaaaattc gtgtgtttct
     1561 agctagtact tgatgttatc tttaaccttt tagtaatttt ttgtcctttt ctttctattt
     1621 ttcatcttac aatgaattat gagcaagttc cttaagtagc atcacacgtg agatgttttt
     1681 tatgatattg actaaatcca atctttacca ttccttaact agtaaaatac aacacatgtt
     1741 aattgataca ttgcttaaca ctgaggttag aaaattttag aaattagttg tccaaatgct
     1801 ttgaaattag aaatctttaa tcccttattt ttttttaaaa tgttttttct cactccaaag
     1861 aaagagaaac tgacatgaaa gctcaaaaga tcatgaatct tactaacttt gtggaactaa
     1921 atgtacatca gaatgtttct gacatgtgaa aatgaaagct cttaattttc ttcttttatt
     1981 tattgagggt ttttgcatgc tatgcattca atttgagtac tttaaagcac ctataaacac
     2041 ttacttacac ttgccttgga gtttatgttt tagtgttttc ttcacatctt ttttggtcaa
     2101 tttgcaggtc tgcagtcgac ggtaccgcgg gcccgggatc cacctagtct agatcccgat
     2161 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     2221 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     2281 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     2341 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     2401 ttactagatc ggggcggccg ctcgctacct taggaccgtt atagttagta atcatggtca
     2461 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     2521 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     2581 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2641 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2701 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2761 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2821 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2881 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2941 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3001 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     3061 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3121 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3181 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3241 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     3301 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3361 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3421 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3481 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3541 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3601 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3661 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3721 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3781 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3841 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3901 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3961 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     4021 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     4081 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     4141 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     4201 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     4261 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4321 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4381 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4441 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4501 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4561 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     4621 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
