LOCUS       40924_38553        4589 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT5-EGFP-C1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Tzfira T, Tian GW, Lacroix B, Vyas S, Li J, Leitner-Dagan Y, 
            Krichevsky A, Taylor T, Vainstein A, Citovsky V.
  TITLE     pSAT vectors: a modular series of plasmids for autofluorescent 
            protein tagging and expression of multiple genes in plants
  JOURNAL   Plant Mol. Biol. 57 (4), 503-516 (2005)
  PUBMED    15821977
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Tzfira T, Lacroix B, Citovsky V.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2004) Department of Biochemistry and Cell Biology,
            State University of New York Stony Brook, 100 Nicolls Rd, Stony 
            Brook, NY 11794, USA
REFERENCE   3  (bases 1 to 4589)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4589)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2005"; volume: "57"; issue: "4"; pages: "503-516"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-NOV-2004) Department of Biochemistry and Cell Biology, State 
            University of New York Stony Brook, 100 Nicolls Rd, Stony Brook, NY 
            11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        505..1176
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1186..1316
                     /note="5' UTR from tobacco etch virus; translational
                     enhancer"
     CDS             1319..2035
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    2036..2101
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      2114..2324
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2368..2384)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2392..2408)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2416..2446)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2461..2482)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2770..3358)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3532..4389)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4390..4494)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcctc gctaccttag gaccgttata
      421 gttaaccggt caacatgtgg agcacgacac acttgtctac tccaaaaata tcaaagatac
      481 agtctcagaa gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct
      541 cctcggattc cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg
      601 tggctcctac aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc
      661 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt
      721 tccaaccacg tcttcaaagc aagtggattg atgtgataac atggtggagc acgacacact
      781 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt
      841 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt
      901 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
      961 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
     1021 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
     1081 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
     1141 ctctatataa ggaagttcat ttcatttgga gaggacgtcg agagttctca acacaacata
     1201 tacaaaacaa acgaatctca agcaatcaag cattctactt ctattgcagc aatttaaatc
     1261 atttctttta aagcaaaagc aattttctga aaattttcac catttacgaa cgatagccat
     1321 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1381 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1441 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1501 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1561 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1621 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1681 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1741 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1801 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1861 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1921 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1981 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg
     2041 actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc
     2101 cacctagtct agagtccgca aaaatcacca gtctctctct acaaatctat ctctctctat
     2161 ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt
     2221 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     2281 tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacgcggcc gctcgctacc
     2341 ttaggaccgt tatagttagt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     2401 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     2461 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     2521 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2581 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2641 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2701 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2761 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2821 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2881 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2941 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
     3001 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3061 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3121 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3181 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     3241 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3301 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3361 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3421 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3481 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3541 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3601 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3661 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3721 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3781 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3841 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3901 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3961 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4021 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4081 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4141 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4201 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4261 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4321 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4381 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4441 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4501 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     4561 aaataggcgt atcacgaggc cctttcgtc
//
