LOCUS       62056_19205        4378 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        497..1168
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1309..1365
                     /label=MCS
                     /note="multiple cloning site"
     CDS             1368..1886
                     /codon_start=1
                     /label=VN173
                     /note="N-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(2157..2173)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2181..2197)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2205..2235)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2250..2271)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2559..3147)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3321..4178)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4179..4283)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccat taccctgtta tccctaaccg
      421 gtcaacatgt ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag
      481 aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat
      541 tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct
      601 acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg
      661 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
      721 cgtcttcaaa gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact
      781 ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa
      841 gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga
      901 agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca
      961 tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     1021 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
     1081 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     1141 aaggaagttc atttcatttg gagaggacgt cgagagttct caacacaaca tatacaaaac
     1201 aaacgaatct caagcaatca agcattctac ttctattgca gcaatttaaa tcatttcttt
     1261 taaagcaaaa gcaattttct gaaaattttc accatttacg aacgatagag atctcgagct
     1321 caagcttcga attctgcagt cgacggtacc gcgggcccgg gatcctgatg gtgagcaagg
     1381 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1441 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1501 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct
     1561 tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     1621 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1681 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1741 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1801 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     1861 acttcaagat ccgccacaac atcgaggact gaagatccac ctagtctaga gtccgcaaaa
     1921 atcaccagtc tctctctaca aatctatctc tctctatttt tctccagaat aatgtgtgag
     1981 tagttcccag ataagggaat tagggttctt atagggtttc gctcatgtgt tgagcatata
     2041 agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc
     2101 ctaaaaccaa aatccagtga cgcggccgca ttaccctgtt atccctagta atcatggtca
     2161 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     2221 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     2281 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2341 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2401 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2461 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2521 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2581 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2641 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2701 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     2761 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2821 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2881 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2941 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     3001 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3061 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3121 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3181 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3241 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3301 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3361 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3421 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3481 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3541 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3601 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3661 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     3721 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     3781 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3841 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3901 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3961 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     4021 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     4081 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     4141 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4201 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4261 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     4321 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
