LOCUS       40924_38543        5214 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT4.35S.RNAi, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Dafny-Yelin M, Chung SM, Frankman EL, Tzfira T.
  TITLE     pSAT RNA interference vectors: a modular series for multiple gene 
            down-regulation in plants
  JOURNAL   Plant Physiol. 145 (4), 1272-1281 (2007)
  PUBMED    17766396
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Dafny-Yelin M, Chung S-M., Frankman EL, Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2007) Department of Molecular, Cellular and 
            Developmental Biology, The University of Michigan, Ann Arbor, MI 
            48109, USA
REFERENCE   3  (bases 1 to 5214)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5214)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1272-1281"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-JUL-2007) Department of Molecular, Cellular and Developmental 
            Biology, The University of Michigan, Ann Arbor, MI 48109, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        497..1168
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     5'UTR           1178..1308
                     /note="translational enhancer; 5' UTR from tobacco etch
                     virus"
     misc_feature    1309..1349
                     /note="multiple cloning site I; MCS I"
     intron          1352..2700
                     /label=chsA intron
                     /note="chalcone synthase A intron from Petunia hybrida"
     misc_feature    2703..2746
                     /note="multiple cloning site II; MCS II"
     regulatory      2747..2957
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2993..3009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3017..3033)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3041..3071)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3086..3107)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3395..3983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4157..5014)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5015..5119)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccat taccctgtta tccctaaccg
      421 gtcaacatgt ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag
      481 aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat
      541 tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct
      601 acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg
      661 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
      721 cgtcttcaaa gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact
      781 ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa
      841 gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga
      901 agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca
      961 tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     1021 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
     1081 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     1141 aaggaagttc atttcatttg gagaggacgt cgagagttct caacacaaca tatacaaaac
     1201 aaacgaatct caagcaatca agcattctac ttctattgca gcaatttaaa tcatttcttt
     1261 taaagcaaaa gcaattttct gaaaattttc accatttacg aacgatagcc atggtccgga
     1321 ctcagatctc gagctcaagc ttcgaattcg tgtaagaatt tcttatgtta cattattaca
     1381 ttcaacgttt tatcttaatt ggctcttcat ttgattgaaa tttgacaatt atttcttgtt
     1441 tttttttttg tcacactctt tttgggttgg ggtggccgac gaattgtggg aaggtagaaa
     1501 gaggggagga cttttgttat actccattag taattactgt ttccgtttca atttatgtga
     1561 caatatttcc tttttagtcg gttccaaaag aaaatgtcag cattataaac aatttaattt
     1621 tgaaattaca attttgccat taataaaatg atttacaacc acaaaagtat ctatgagcct
     1681 gtttgggtgg gcttataagc agcttatttt aagtggctta taagtcaaaa agtgacactt
     1741 tttgagaagt tagaaaatcc taacttctca aaaagtagct tttaagccac ttatgactta
     1801 taagtccaaa aatttttaag ttaccaaaca tatattaatg ggtttataag cttataagcc
     1861 acttttaagc tcacccaaac gggttctatg tctcacttta gactacaaat tttaaaagtc
     1921 ttcatttatt tcttaatctc cgtggcgagt caaactataa cacataaagt gaaacggagg
     1981 gaataagatg gagtcataaa ctaatccaaa tctatactct ctccgttaat ttgtttttta
     2041 gtttgatttg gtacattaat aaaacagatt tttcgaaggt tataaacaca gacagatgtt
     2101 tcccagcgag ctagcaaaat tccaagattt ctgtcgaaaa ttcgtgtgtt tctagctagt
     2161 acttgatgtt atctttaacc ttttagtaat tttttgtcct tttctttcta tttttcatct
     2221 tacaatgaat tatgagcaag ttccttaagt agcatcacac gtgagatgtt ttttatgata
     2281 ttgactaaat ccaatcttta ccattcctta actagtaaaa tacaacacat gttaattgat
     2341 acattgctta acactgaggt tagaaaattt tagaaattag ttgtccaaat gctttgaaat
     2401 tagaaatctt taatccctta ttttttttta aaatgttttt tctcactcca aagaaagaga
     2461 aactgacatg aaagctcaaa agatcatgaa tcttactaac tttgtggaac taaatgtaca
     2521 tcagaatgtt tctgacatgt gaaaatgaaa gctcttaatt ttcttctttt atttattgag
     2581 ggtttttgca tgctatgcat tcaatttgag tactttaaag cacctataaa cacttactta
     2641 cacttgcctt ggagtttatg ttttagtgtt ttcttcacat cttttttggt caatttgcag
     2701 gtctgcagtc gacggtaccg cgggcccggg atccacctag tctagagtcc gcaaaaatca
     2761 ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg tgtgagtagt
     2821 tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag catataagaa
     2881 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     2941 aaccaaaatc cagtgacgcg gccgcattac cctgttatcc ctagtaatca tggtcatagc
     3001 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     3061 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     3121 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     3181 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     3241 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     3301 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3361 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3421 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3481 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3541 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     3601 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3661 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3721 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3781 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     3841 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3901 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3961 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4021 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     4081 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4141 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4201 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4261 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4321 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4381 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4441 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4501 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4561 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4621 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4681 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4741 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4801 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4861 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4921 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4981 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     5041 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5101 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     5161 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
