LOCUS       40924_38538        4573 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT4-EGFP-C1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Tzfira T, Tian GW, Lacroix B, Vyas S, Li J, Leitner-Dagan Y, 
            Krichevsky A, Taylor T, Vainstein A, Citovsky V.
  TITLE     pSAT vectors: a modular series of plasmids for autofluorescent 
            protein tagging and expression of multiple genes in plants
  JOURNAL   Plant Mol. Biol. 57 (4), 503-516 (2005)
  PUBMED    15821977
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Tzfira T, Lacroix B, Citovsky V.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2004) Department of Biochemistry and Cell Biology,
            State University of New York Stony Brook, 100 Nicolls Rd, Stony 
            Brook, NY 11794, USA
REFERENCE   3  (bases 1 to 4573)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4573)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2005"; volume: "57"; issue: "4"; pages: "503-516"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-NOV-2004) Department of Biochemistry and Cell Biology, State 
            University of New York Stony Brook, 100 Nicolls Rd, Stony Brook, NY 
            11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        497..1168
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1178..1308
                     /note="5' UTR from tobacco etch virus; translational
                     enhancer"
     CDS             1311..2027
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    2028..2093
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      2106..2316
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2352..2368)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2376..2392)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2400..2430)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2445..2466)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2754..3342)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3516..4373)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4374..4478)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccat taccctgtta tccctaaccg
      421 gtcaacatgt ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag
      481 aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat
      541 tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct
      601 acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg
      661 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
      721 cgtcttcaaa gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact
      781 ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa
      841 gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga
      901 agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca
      961 tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     1021 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
     1081 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     1141 aaggaagttc atttcatttg gagaggacgt cgagagttct caacacaaca tatacaaaac
     1201 aaacgaatct caagcaatca agcattctac ttctattgca gcaatttaaa tcatttcttt
     1261 taaagcaaaa gcaattttct gaaaattttc accatttacg aacgatagcc atggtgagca
     1321 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1381 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     1441 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1501 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     1561 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1621 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1681 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     1741 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     1801 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1861 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1921 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1981 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat
     2041 ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccacctagt
     2101 ctagagtccg caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc
     2161 agaataatgt gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca
     2221 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     2281 taaaatttct aattcctaaa accaaaatcc agtgacgcgg ccgcattacc ctgttatccc
     2341 tagtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     2401 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     2461 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     2521 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     2581 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2641 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2701 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2761 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2821 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2881 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2941 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3001 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3061 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3121 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3181 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3241 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3301 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3361 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3421 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3481 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3541 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3601 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3661 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3721 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3781 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3841 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3901 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     3961 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4021 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     4081 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     4141 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4201 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4261 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4321 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4381 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4441 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4501 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     4561 aggccctttc gtc
//
