LOCUS       40924_38533        3860 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT4-35SP-MCS-35ST, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Chung SM, Frankman EL, Tzfira T.
  TITLE     A versatile vector system for multiple gene expression in plants
  JOURNAL   Trends Plant Sci. 10 (8), 357-361 (2005)
  PUBMED    15993643
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Chung S-M., Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2005) Department of Biochemistry and Cell Biology,
            State University of New York at Stony Brook, Stony Brook, NY 11794, 
            USA
REFERENCE   3  (bases 1 to 3860)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3860)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Trends 
            Plant Sci."; date: "2005"; volume: "10"; issue: "8"; pages: 
            "357-361"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-APR-2005) Department of Biochemistry and Cell Biology, State 
            University of New York at Stony Brook, Stony Brook, NY 11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        497..1168
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     regulatory      1178..1308
                     /note="translational enhancer 5'-UTR from tobacco etch
                     virus"
                     /regulatory_class="enhancer"
     misc_feature    1315..1380
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1393..1603
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(1639..1655)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1663..1679)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1687..1717)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1732..1753)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2041..2629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2803..3660)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3661..3765)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccat taccctgtta tccctaaccg
      421 gtcaacatgt ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag
      481 aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat
      541 tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct
      601 acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg
      661 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca
      721 cgtcttcaaa gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact
      781 ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa
      841 gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga
      901 agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca
      961 tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     1021 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
     1081 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     1141 aaggaagttc atttcatttg gagaggacgt cgagagttct caacacaaca tatacaaaac
     1201 aaacgaatct caagcaatca agcattctac ttctattgca gcaatttaaa tcatttcttt
     1261 taaagcaaaa gcaattttct gaaaattttc accatttacg aacgatagcc atggtccgga
     1321 ctcagatctc gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc
     1381 acctagtcta gagtccgcaa aaatcaccag tctctctcta caaatctatc tctctctatt
     1441 tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt
     1501 tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt
     1561 ctatcaataa aatttctaat tcctaaaacc aaaatccagt gacgcggccg cattaccctg
     1621 ttatccctag taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1681 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1741 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1801 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1861 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1921 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1981 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2041 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2101 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2161 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2221 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2281 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2341 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2401 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2461 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     2521 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2581 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2641 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2701 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2761 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2821 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2881 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2941 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3001 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3061 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3121 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3181 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3241 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3301 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3361 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3421 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3481 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3541 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3601 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3661 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3721 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3781 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     3841 tatcacgagg ccctttcgtc
//
