LOCUS       40924_38528        3400 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT3A-masP-MCS-masT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Chung SM, Frankman EL, Tzfira T.
  TITLE     A versatile vector system for multiple gene expression in plants
  JOURNAL   Trends Plant Sci. 10 (8), 357-361 (2005)
  PUBMED    15993643
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Chung S-M., Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2005) Department of Biochemistry and Cell Biology,
            State University of New York at Stony Brook, Stony Brook, NY 11794, 
            USA
REFERENCE   3  (bases 1 to 3400)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3400)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Trends 
            Plant Sci."; date: "2005"; volume: "10"; issue: "8"; pages: 
            "357-361"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-APR-2005) Department of Biochemistry and Cell Biology, State 
            University of New York at Stony Brook, Stony Brook, NY 11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        426..806
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    808..873
                     /label=MCS
                     /note="multiple cloning site"
     terminator      889..1141
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     primer_bind     complement(1179..1195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1203..1219)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1227..1257)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1272..1293)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1581..2169)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2343..3200)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3201..3305)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccgc taccttaaga gagaccggtt
      421 gagatttttc aaatcagtgc gcaagacgtg acgtaagtat ctgagctagt ttttattttt
      481 ctactaattt ggtcgtttat ttcggcgtgt aggacatggc aaccgggcct gaatttcgcg
      541 ggtattctgt ttctattcca actttttctt gatccgcagc cattaacgac ttttgaatag
      601 atacgctgac acgccaagcc tcgctagtca aaagtgtacc aaacaacgct ttacagcaag
      661 aacggaatgc gcgtgacgct cgcggtgacg ccatttcgcc ttttcagaaa tggataaata
      721 gccttgcttc ctattatatc ttcccaaatt accaatacat tacactagca tctgaatttc
      781 ataaccaatc tcgatacacc aaatcgatcc ggactcagat ctcgagctca agcttcgaat
      841 tctgcagtcg acggtaccgc gggcccggga tccacctagt ctagaaatct tggactccca
      901 tgttggcaaa ggcaaccaaa caaacaatga atgatccgct cctgcatatg gggcggtttg
      961 agtatttcaa ctgccatttg ggctgaattg aagacatgct cctgtcagaa attccgtgat
     1021 cttactcaat attcagtaat ctcggccaat atcctaaatg tgcgtggctt tatctgtctt
     1081 tgtattgttt catcaattca tgtaacgttt gcttttctta tgaattttca aataaattat
     1141 cagatcgcgg ccgcgctacc ttaagagagg taatcatggt catagctgtt tcctgtgtga
     1201 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     1261 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     1321 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1381 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1501 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1561 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1621 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1681 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1741 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     1801 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1861 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1921 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1981 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     2041 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2101 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2161 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2221 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2281 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2341 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2401 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2461 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     2521 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2581 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2641 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2701 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2761 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2821 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2881 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     2941 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3001 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3061 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3121 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3181 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3241 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3301 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     3361 ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
