LOCUS       40924_38523        4760 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT3.masP.RNAi, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Dafny-Yelin M, Chung SM, Frankman EL, Tzfira T.
  TITLE     pSAT RNA interference vectors: a modular series for multiple gene 
            down-regulation in plants
  JOURNAL   Plant Physiol. 145 (4), 1272-1281 (2007)
  PUBMED    17766396
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Dafny-Yelin M, Chung S-M., Frankman EL, Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2007) Department of Molecular, Cellular and 
            Developmental Biology, The University of Michigan, Ann Arbor, MI 
            48109, USA
REFERENCE   3  (bases 1 to 4760)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4760)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2007"; volume: "145"; issue: "4"; pages: 
            "1272-1281"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-JUL-2007) Department of Molecular, Cellular and Developmental 
            Biology, The University of Michigan, Ann Arbor, MI 48109, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        426..806
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    808..848
                     /note="multiple cloning site I; MCS I"
     intron          851..2199
                     /label=chsA intron
                     /note="chalcone synthase A intron from Petunia hybrida"
     misc_feature    2202..2245
                     /note="multiple cloning site II; MCS II"
     terminator      2249..2501
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     primer_bind     complement(2539..2555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2563..2579)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2587..2617)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2632..2653)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2941..3529)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3703..4560)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4561..4665)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccgc taccttaaga gagaccggtt
      421 gagatttttc aaatcagtgc gcaagacgtg acgtaagtat ctgagctagt ttttattttt
      481 ctactaattt ggtcgtttat ttcggcgtgt aggacatggc aaccgggcct gaatttcgcg
      541 ggtattctgt ttctattcca actttttctt gatccgcagc cattaacgac ttttgaatag
      601 atacgctgac acgccaagcc tcgctagtca aaagtgtacc aaacaacgct ttacagcaag
      661 aacggaatgc gcgtgacgct cgcggtgacg ccatttcgcc ttttcagaaa tggataaata
      721 gccttgcttc ctattatatc ttcccaaatt accaatacat tacactagca tctgaatttc
      781 ataaccaatc tcgatacacc aaatcgacca tggtccggac tcagatctcg agctcaagct
      841 tcgaattcgt gtaagaattt cttatgttac attattacat tcaacgtttt atcttaattg
      901 gctcttcatt tgattgaaat ttgacaatta tttcttgttt ttttttttgt cacactcttt
      961 ttgggttggg gtggccgacg aattgtggga aggtagaaag aggggaggac ttttgttata
     1021 ctccattagt aattactgtt tccgtttcaa tttatgtgac aatatttcct ttttagtcgg
     1081 ttccaaaaga aaatgtcagc attataaaca atttaatttt gaaattacaa ttttgccatt
     1141 aataaaatga tttacaacca caaaagtatc tatgagcctg tttgggtggg cttataagca
     1201 gcttatttta agtggcttat aagtcaaaaa gtgacanttt ttgagaagtt agaaaatcct
     1261 aacttctcaa aaagtagctt ttaagccact tatgacttat aagtccaaaa atttttaagt
     1321 taccaaacat atattaatgg gtttataagc ttataagcca cttttaagct cacccaaacg
     1381 ggttctatgt ctcactttag actacaaatt ttaaaagtct tcatttattt cttaatctcc
     1441 gtggcgagtn aaactataac acataaagtg aaacggaggg aataagatgg agtcataaac
     1501 taatccaaat ctatactctc tccgttaatt tgttttttag tttgatttgg tacattaata
     1561 aaacagattt ttcgaaggtt ataaacacag acagatgttt cccagcgagc tagcaaaatt
     1621 ccaagatttc tgtcgaaaat tcgtgtgttt ctagctagta cttgatgtta tctttaacct
     1681 tttagtaatt ttttgtcctt ttctttctat ttttcatctt acaatgaatt atgagcaagt
     1741 tccttaagta gcatcacacg tgagatgttt tttatgatat tgactaaatc caatctttac
     1801 cattccttaa ctagtaaaat acaacacatg ttaattgata cattgcttaa cactgaggtt
     1861 agaaaatttt agaaattagt tgtccaaatg ctttgaaatt agaaatcttt aatcccttat
     1921 ttttttttaa aatgtttttt ctcactccaa agaaagagaa actgacatga aagctcaaaa
     1981 gatcatgaat cttactaact ttgtggaact aaatgtacat cagaatgttt ctgacatgtg
     2041 aaaatgaaag ctcttaattt tcttctttta tttattgagg gtttttgcat gctatgcatt
     2101 caatttgagt actttaaagc acctataaac acttacttac acttgccttg gagtttatgt
     2161 tttagtgttt tcttcacatc ttttttggtc aatttgcagg tctgcagtcg acggtaccgc
     2221 gggcccggga tccacctagt ctagaaatct tggactccca tgttggcaaa ggcaaccaaa
     2281 caaacaatga atgatccgct cctgcatatg gggcggtttg agtatttcaa ctgccatttg
     2341 ggctgaattg aagacatgct cctgtcagaa attccgtgat cttactcaat attcagtaat
     2401 ctcggccaat atcctaaatg tgcgtggctt tatctgtctt tgtattgttt catcaattca
     2461 tgtaacgttt gcttttctta tgaattttca aataaattat cagatcgcgg ccgcgctacc
     2521 ttaagagagg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2581 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2641 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2701 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2761 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2821 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2881 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2941 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3001 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3061 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3121 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3181 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3241 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3301 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3361 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     3421 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3481 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3541 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3601 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3661 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3721 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3781 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3841 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3901 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3961 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4021 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4081 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4141 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4201 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4261 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4321 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4381 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4441 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4501 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4561 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4621 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4681 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     4741 tatcacgagg ccctttcgtc
//
