LOCUS       40924_38513        4567 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT3-EGFP-C1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Tzfira T, Tian GW, Lacroix B, Vyas S, Li J, Leitner-Dagan Y, 
            Krichevsky A, Taylor T, Vainstein A, Citovsky V.
  TITLE     pSAT vectors: a modular series of plasmids for autofluorescent 
            protein tagging and expression of multiple genes in plants
  JOURNAL   Plant Mol. Biol. 57 (4), 503-516 (2005)
  PUBMED    15821977
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Tzfira T, Lacroix B, Citovsky V.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2004) Department of Biochemistry and Cell Biology,
            State University of New York Stony Brook, 100 Nicolls Rd, Stony 
            Brook, NY 11794, USA
REFERENCE   3  (bases 1 to 4567)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4567)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2005"; volume: "57"; issue: "4"; pages: "503-516"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-NOV-2004) Department of Biochemistry and Cell Biology, State 
            University of New York Stony Brook, 100 Nicolls Rd, Stony Brook, NY 
            11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        494..1165
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1175..1305
                     /note="5' UTR from tobacco etch virus; translational
                     enhancer"
     CDS             1308..2024
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    2025..2090
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      2103..2313
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2346..2362)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2370..2386)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2394..2424)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2439..2460)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2748..3336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3510..4367)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4368..4472)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccgc taccttaaga gagaccggtc
      421 aacatgtgga gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag
      481 accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc
      541 attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca
      601 aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc
      661 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
      721 cttcaaagca agtggattga tgtgataaca tggtggagca cgacacactt gtctactcca
      781 aaaatatcaa agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg
      841 taatatccgg aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga
      901 tagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg
      961 ttgaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
     1021 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca
     1081 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
     1141 gaagttcatt tcatttggag aggacgtcga gagttctcaa cacaacatat acaaaacaaa
     1201 cgaatctcaa gcaatcaagc attctacttc tattgcagca atttaaatca tttcttttaa
     1261 agcaaaagca attttctgaa aattttcacc atttacgaac gatagccatg gtgagcaagg
     1321 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1381 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1441 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1501 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     1561 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1621 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1681 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1741 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     1801 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     1861 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     1921 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1981 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc
     2041 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc acctagtcta
     2101 gagtccgcaa aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga
     2161 ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt
     2221 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
     2281 aatttctaat tcctaaaacc aaaatccagt gacgcggccg cgctacctta agagaggtaa
     2341 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     2401 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     2461 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     2521 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2581 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2641 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2701 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2761 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2821 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2881 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2941 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3001 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3061 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3121 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3181 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3241 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3301 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3361 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3421 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3481 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3541 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3601 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3661 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3721 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3781 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3841 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3901 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3961 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4021 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4081 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4141 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4201 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4261 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4321 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4381 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4441 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4501 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     4561 tttcgtc
//
