LOCUS       40924_38498        4560 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT2-EGFP-C1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Tzfira T, Tian GW, Lacroix B, Vyas S, Li J, Leitner-Dagan Y, 
            Krichevsky A, Taylor T, Vainstein A, Citovsky V.
  TITLE     pSAT vectors: a modular series of plasmids for autofluorescent 
            protein tagging and expression of multiple genes in plants
  JOURNAL   Plant Mol. Biol. 57 (4), 503-516 (2005)
  PUBMED    15821977
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Tzfira T, Lacroix B, Citovsky V.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2004) Department of Biochemistry and Cell Biology,
            State University of New York Stony Brook, 100 Nicolls Rd, Stony 
            Brook, NY 11794, USA
REFERENCE   3  (bases 1 to 4560)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4560)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2005"; volume: "57"; issue: "4"; pages: "503-516"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-NOV-2004) Department of Biochemistry and Cell Biology, State 
            University of New York Stony Brook, 100 Nicolls Rd, Stony Brook, NY 
            11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        494..1165
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1175..1305
                     /note="5' UTR from tobacco etch virus; translational
                     enhancer"
     CDS             1308..2024
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    2025..2090
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      2103..2313
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2339..2355)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2363..2379)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2387..2417)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2432..2453)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2741..3329)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3503..4360)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4361..4465)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccgc taccttaaga gagaccggtc
      421 aacatgtgga gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag
      481 accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc
      541 attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca
      601 aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc
      661 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
      721 cttcaaagca agtggattga tgtgataaca tggtggagca cgacacactt gtctactcca
      781 aaaatatcaa agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg
      841 taatatccgg aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga
      901 tagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg
      961 ttgaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
     1021 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gatatctcca
     1081 ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc tctatataag
     1141 gaagttcatt tcatttggag aggacgtcga gagttctcaa cacaacatat acaaaacaaa
     1201 cgaatctcaa gcaatcaagc attctacttc tattgcagca atttaaatca tttcttttaa
     1261 agcaaaagca attttctgaa aattttcacc atttacgaac gatagccatg gtgagcaagg
     1321 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1381 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1441 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1501 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     1561 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1621 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1681 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1741 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     1801 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     1861 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     1921 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     1981 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc
     2041 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc acctagtcta
     2101 gagtccgcaa aaatcaccag tctctctcta caaatctatc tctctctatt tttctccaga
     2161 ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt
     2221 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
     2281 aatttctaat tcctaaaacc aaaatccagt gacgcggccg cggcgcgccg taatcatggt
     2341 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     2401 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     2461 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     2521 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     2581 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2641 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2701 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2761 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2821 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2881 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     2941 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3001 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     3061 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     3121 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     3181 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     3241 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     3301 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3361 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3421 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3481 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3541 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3601 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3661 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3721 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3781 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3841 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3901 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3961 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4021 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4081 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     4141 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     4201 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     4261 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     4321 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4381 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     4441 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     4501 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
