LOCUS       40924_38493        4603 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT1A-ocsAocsP-nptII-ocsT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Chung SM, Frankman EL, Tzfira T.
  TITLE     A versatile vector system for multiple gene expression in plants
  JOURNAL   Trends Plant Sci. 10 (8), 357-361 (2005)
  PUBMED    15993643
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Chung S-M., Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2005) Department of Biochemistry and Cell Biology,
            State University of New York at Stony Brook, Stony Brook, NY 11794, 
            USA
REFERENCE   3  (bases 1 to 4603)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4603)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Trends 
            Plant Sci."; date: "2005"; volume: "10"; issue: "8"; pages: 
            "357-361"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-APR-2005) Department of Biochemistry and Cell Biology, State 
            University of New York at Stony Brook, Stony Brook, NY 11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    413..625
                     /label=OCS activator
                     /note="OCS activator"
     regulatory      632..772
                     /label=OCS promoter
                     /note="OCS promoter"
                     /regulatory_class="promoter"
     CDS             821..1609
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     terminator      1647..2354
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(2382..2398)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2406..2422)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2430..2460)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2475..2496)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2784..3372)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3546..4403)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4404..4508)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccgg cgcgccaccg ggctgaaagc
      421 gacgttggat gttaacatct acaaattgcc ttttcttatc gaccatgtac gtaagcgctt
      481 acgtttttgg tggacccttg aggaaactgg tagctgttgt gggcctgtgg tctcaagatg
      541 gatcattaat ttccaccttc acctacgatg gggggcatcg caccggtgag taatattgta
      601 cggctaagag cgaatttggc ctgtaagatc ctttttaccg acaactcatc cacattgatg
      661 gtaggcagaa agttaaagga ttatcgcaag tcaatacttg cccattcatt gatctattta
      721 aaggtgtggc ctcaaggata atcgccaaac cattatattt gcaatctacc aatccggact
      781 cagatctcga gctcaagctt cgaattctgc agtcgacatg attgaacaag atggattgca
      841 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      901 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      961 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
     1021 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     1081 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     1141 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     1201 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     1261 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     1321 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca
     1381 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1441 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1501 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1561 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaggatccac
     1621 ctagtctaga actagtccct agagtcctgc tttaatgaga tatgcgagac gcctatgatc
     1681 gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct
     1741 cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat
     1801 ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa
     1861 tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc
     1921 gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag
     1981 aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca
     2041 ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc
     2101 ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct
     2161 accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc
     2221 tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc
     2281 aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg
     2341 tcgaggctca gcaggagcgg ccgcggcgcg ccgtaatcat ggtcatagct gtttcctgtg
     2401 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     2461 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     2521 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     2581 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2641 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2701 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2761 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2821 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2881 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2941 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     3001 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3061 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3121 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3181 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     3241 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3301 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3361 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3421 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3481 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3541 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3601 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3661 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3721 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3781 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3841 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3901 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3961 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4021 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4081 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4141 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     4201 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4261 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4321 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4381 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4441 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4501 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     4561 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
