LOCUS       40924_38483        4823 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT1A-ocsAocsP-hpt-ocsT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Chung SM, Frankman EL, Tzfira T.
  TITLE     A versatile vector system for multiple gene expression in plants
  JOURNAL   Trends Plant Sci. 10 (8), 357-361 (2005)
  PUBMED    15993643
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Chung S-M., Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2005) Department of Biochemistry and Cell Biology,
            State University of New York at Stony Brook, Stony Brook, NY 11794, 
            USA
REFERENCE   3  (bases 1 to 4823)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4823)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Trends 
            Plant Sci."; date: "2005"; volume: "10"; issue: "8"; pages: 
            "357-361"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-APR-2005) Department of Biochemistry and Cell Biology, State 
            University of New York at Stony Brook, Stony Brook, NY 11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    413..625
                     /label=OCS activator
                     /note="OCS activator"
     regulatory      632..772
                     /label=OCS promoter
                     /note="OCS promoter"
                     /regulatory_class="promoter"
     CDS             801..1823
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     terminator      1867..2574
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(2602..2618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2626..2642)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2650..2680)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2695..2716)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3004..3592)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3766..4623)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4624..4728)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccgg cgcgccaccg ggctgaaagc
      421 gacgttggat gttaacatct acaaattgcc ttttcttatc gaccatgtac gtaagcgctt
      481 acgtttttgg tggacccttg aggaaactgg tagctgttgt gggcctgtgg tctcaagatg
      541 gatcattaat ttccaccttc acctacgatg gggggcatcg caccggtgag taatattgta
      601 cggctaagag cgaatttggc ctgtaagatc ctttttaccg acaactcatc cacattgatg
      661 gtaggcagaa agttaaagga ttatcgcaag tcaatacttg cccattcatt gatctattta
      721 aaggtgtggc ctcaaggata atcgccaaac cattatattt gcaatctacc aatccggact
      781 cagatctcga gctcaagctt atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
      841 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
      901 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
      961 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     1021 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     1081 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     1141 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     1201 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     1261 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     1321 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     1381 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     1441 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     1501 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     1561 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     1621 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     1681 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     1741 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     1801 gcactcgtcc gagggcaaag gaatagggat ccacctagtc tagatctaga actagtccct
     1861 agagtcctgc tttaatgaga tatgcgagac gcctatgatc gcatgatatt tgctttcaat
     1921 tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct cagatcctta ccgccggttt
     1981 cggttcattc taatgaatat atcacccgtt actatcgtat ttttatgaat aatattctcc
     2041 gttcaattta ctgattgtac cctactactt atatgtacaa tattaaaatg aaaacaatat
     2101 attgtgctga ataggtttat agcgacatct atgatagagc gccacaataa caaacaattg
     2161 cgttttatta ttacaaatcc aattttaaaa aaagcggcag aaccggtcaa acctaaaaga
     2221 ctgattacat aaatcttatt caaatttcaa aagtgcccca ggggctagta tctacgacac
     2281 accgagcggc gaactaataa cgctcactga agggaactcc ggttccccgc cggcgcgcat
     2341 gggtgagatt ccttgaagtt gagtattggc cgtccgctct accgaaagtt acgggcacca
     2401 ttcaacccgg tccagcacgg cggccgggta accgacttgc tgccccgaga attatgcagc
     2461 atttttttgg tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac agtgacgaca
     2521 aatcgttggg cgggtccagg gcgaattttg cgacaacatg tcgaggctca gcaggagcgg
     2581 ccgcggcgcg ccgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     2641 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     2701 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2761 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2821 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2881 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2941 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     3001 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     3061 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     3121 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     3181 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     3241 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     3301 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     3361 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     3421 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     3481 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3541 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3601 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3661 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3721 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3781 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3841 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3901 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3961 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4021 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4081 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4141 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4201 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4261 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4321 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     4381 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     4441 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4501 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4561 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4621 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4681 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4741 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     4801 gcgtatcacg aggccctttc gtc
//
