LOCUS       40924_38478        4340 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT1A-ocsAocsP-bar-ocsT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Chung SM, Frankman EL, Tzfira T.
  TITLE     A versatile vector system for multiple gene expression in plants
  JOURNAL   Trends Plant Sci. 10 (8), 357-361 (2005)
  PUBMED    15993643
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Chung S-M., Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2005) Department of Biochemistry and Cell Biology,
            State University of New York at Stony Brook, Stony Brook, NY 11794, 
            USA
REFERENCE   3  (bases 1 to 4340)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4340)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Trends 
            Plant Sci."; date: "2005"; volume: "10"; issue: "8"; pages: 
            "357-361"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-APR-2005) Department of Biochemistry and Cell Biology, State 
            University of New York at Stony Brook, Stony Brook, NY 11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    413..625
                     /label=OCS activator
                     /note="OCS activator"
     regulatory      632..772
                     /label=OCS promoter
                     /note="OCS promoter"
                     /regulatory_class="promoter"
     CDS             794..1342
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     terminator      1384..2091
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(2119..2135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2143..2159)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2167..2197)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2212..2233)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2521..3109)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3283..4140)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4141..4245)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccgg cgcgccaccg ggctgaaagc
      421 gacgttggat gttaacatct acaaattgcc ttttcttatc gaccatgtac gtaagcgctt
      481 acgtttttgg tggacccttg aggaaactgg tagctgttgt gggcctgtgg tctcaagatg
      541 gatcattaat ttccaccttc acctacgatg gggggcatcg caccggtgag taatattgta
      601 cggctaagag cgaatttggc ctgtaagatc ctttttaccg acaactcatc cacattgatg
      661 gtaggcagaa agttaaagga ttatcgcaag tcaatacttg cccattcatt gatctattta
      721 aaggtgtggc ctcaaggata atcgccaaac cattatattt gcaatctacc aatccggact
      781 cagatctcga gctatgagcc cagaacgacg cccggccgac atccgccgtg ccaccgaggc
      841 ggacatgccg gcggtctgca ccatcgtcaa ccactacatc gagacaagca cggtcaactt
      901 ccgtaccgag ccgcaggaac cgcaggagtg gacggacgac ctcgtccgtc tgcgggagcg
      961 ctatccctgg ctcgtcgccg aggtggacgg cgaggtcgcc ggcatcgcct acgcgggccc
     1021 ctggaaggca cgcaacgcct acgactggac ggccgagtcg accgtgtacg tctccccccg
     1081 ccaccagcgg acgggactgg gctccacgct ctacacccac ctgctgaagt ccctggaggc
     1141 acagggcttc aagagcgtgg tcgctgtcat cgggctgccc aacgacccga gcgtgcgcat
     1201 gcacgaggcg ctcggatatg ccccccgcgg catgctgcgg gcggccggct tcaagcacgg
     1261 gaactggcat gacgtgggtt tctggcagct ggacttcagc ctgccggtac cgccccgtcc
     1321 ggtcctgccc gtcaccgaga tctgacccgg gatccaccta gtctagaact agtccctaga
     1381 gtcctgcttt aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct
     1441 gttgtgcacg ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg
     1501 ttcattctaa tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt
     1561 caatttactg attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt
     1621 gtgctgaata ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt
     1681 tttattatta caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg
     1741 attacataaa tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc
     1801 gagcggcgaa ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg
     1861 tgagattcct tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc
     1921 aacccggtcc agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt
     1981 tttttggtgt atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat
     2041 cgttgggcgg gtccagggcg aattttgcga caacatgtcg aggctcagca ggagcggccg
     2101 cggcgcgccg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2161 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2221 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2281 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2341 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2401 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2461 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2521 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2581 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2641 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2701 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2761 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2821 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2881 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2941 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     3001 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3061 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3121 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3181 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3241 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3301 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3361 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3421 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3481 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3541 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3601 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3661 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3721 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3781 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3841 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3901 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3961 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4021 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4081 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4141 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4201 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4261 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     4321 tatcacgagg ccctttcgtc
//
