LOCUS       40924_38488        3823 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT1A-ocsAocsP-MCS-ocsT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Chung SM, Frankman EL, Tzfira T.
  TITLE     A versatile vector system for multiple gene expression in plants
  JOURNAL   Trends Plant Sci. 10 (8), 357-361 (2005)
  PUBMED    15993643
REFERENCE   2  (bases 1 to 3823)
  AUTHORS   Chung S-M., Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2005) Department of Biochemistry and Cell Biology,
            State University of New York at Stony Brook, Stony Brook, NY 11794, 
            USA
REFERENCE   3  (bases 1 to 3823)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3823)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Trends 
            Plant Sci."; date: "2005"; volume: "10"; issue: "8"; pages: 
            "357-361"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-APR-2005) Department of Biochemistry and Cell Biology, State 
            University of New York at Stony Brook, Stony Brook, NY 11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    413..625
                     /label=OCS activator
                     /note="OCS activator"
     regulatory      632..772
                     /label=OCS promoter
                     /note="OCS promoter"
                     /regulatory_class="promoter"
     misc_feature    773..838
                     /label=MCS
                     /note="multiple cloning site"
     terminator      867..1574
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(1602..1618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1626..1642)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1650..1680)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1695..1716)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2004..2592)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2766..3623)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3624..3728)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccgg cgcgccaccg ggctgaaagc
      421 gacgttggat gttaacatct acaaattgcc ttttcttatc gaccatgtac gtaagcgctt
      481 acgtttttgg tggacccttg aggaaactgg tagctgttgt gggcctgtgg tctcaagatg
      541 gatcattaat ttccaccttc acctacgatg gggggcatcg caccggtgag taatattgta
      601 cggctaagag cgaatttggc ctgtaagatc ctttttaccg acaactcatc cacattgatg
      661 gtaggcagaa agttaaagga ttatcgcaag tcaatacttg cccattcatt gatctattta
      721 aaggtgtggc ctcaaggata atcgccaaac cattatattt gcaatctacc aatccggact
      781 cagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc cgggatccac
      841 ctagtctaga actagtccct agagtcctgc tttaatgaga tatgcgagac gcctatgatc
      901 gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct
      961 cagatcctta ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat
     1021 ttttatgaat aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa
     1081 tattaaaatg aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc
     1141 gccacaataa caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag
     1201 aaccggtcaa acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca
     1261 ggggctagta tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc
     1321 ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct
     1381 accgaaagtt acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc
     1441 tgccccgaga attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc
     1501 aaaccttgac agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg
     1561 tcgaggctca gcaggagcgg ccgcggcgcg ccgtaatcat ggtcatagct gtttcctgtg
     1621 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1681 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1741 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1801 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1861 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1921 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1981 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2041 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2101 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2161 tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     2221 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2281 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2341 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2401 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     2461 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2521 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2581 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2641 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2701 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2761 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2821 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2881 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2941 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3001 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3061 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3121 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3181 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3241 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3301 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3361 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3421 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3481 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3541 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3601 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3661 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3721 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     3781 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
