LOCUS       40924_38473        3829 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT1-ocsAocsP-MCS-ocsT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Chung SM, Frankman EL, Tzfira T.
  TITLE     A versatile vector system for multiple gene expression in plants
  JOURNAL   Trends Plant Sci. 10 (8), 357-361 (2005)
  PUBMED    15993643
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Chung S-M., Tzfira T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2005) Department of Biochemistry and Cell Biology,
            State University of New York at Stony Brook, Stony Brook, NY 11794, 
            USA
REFERENCE   3  (bases 1 to 3829)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3829)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Trends 
            Plant Sci."; date: "2005"; volume: "10"; issue: "8"; pages: 
            "357-361"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-APR-2005) Department of Biochemistry and Cell Biology, State 
            University of New York at Stony Brook, Stony Brook, NY 11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    413..625
                     /label=OCS activator
                     /note="OCS activator"
     regulatory      632..772
                     /label=OCS promoter
                     /note="OCS promoter"
                     /regulatory_class="promoter"
     misc_feature    779..844
                     /label=MCS
                     /note="multiple cloning site"
     terminator      873..1580
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(1608..1624)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1632..1648)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1656..1686)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1701..1722)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2010..2598)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2772..3629)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3630..3734)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccgg cgcgccaccg ggctgaaagc
      421 gacgttggat gttaacatct acaaattgcc ttttcttatc gaccatgtac gtaagcgctt
      481 acgtttttgg tggacccttg aggaaactgg tagctgttgt gggcctgtgg tctcaagatg
      541 gatcattaat ttccaccttc acctacgatg gggggcatcg caccggtgag taatattgta
      601 cggctaagag cgaatttggc ctgtaagatc ctttttaccg acaactcatc cacattgatg
      661 gtaggcagaa agttaaagga ttatcgcaag tcaatacttg cccattcatt gatctattta
      721 aaggtgtggc ctcaaggata atcgccaaac cattatattt gcaatctacc aaccatggtc
      781 cggactcaga tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg
      841 atccacctag tctagaacta gtccctagag tcctgcttta atgagatatg cgagacgcct
      901 atgatcgcat gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt
      961 gtagctcaga tccttaccgc cggtttcggt tcattctaat gaatatatca cccgttacta
     1021 tcgtattttt atgaataata ttctccgttc aatttactga ttgtacccta ctacttatat
     1081 gtacaatatt aaaatgaaaa caatatattg tgctgaatag gtttatagcg acatctatga
     1141 tagagcgcca caataacaaa caattgcgtt ttattattac aaatccaatt ttaaaaaaag
     1201 cggcagaacc ggtcaaacct aaaagactga ttacataaat cttattcaaa tttcaaaagt
     1261 gccccagggg ctagtatcta cgacacaccg agcggcgaac taataacgct cactgaaggg
     1321 aactccggtt ccccgccggc gcgcatgggt gagattcctt gaagttgagt attggccgtc
     1381 cgctctaccg aaagttacgg gcaccattca acccggtcca gcacggcggc cgggtaaccg
     1441 acttgctgcc ccgagaatta tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg
     1501 caggtcaaac cttgacagtg acgacaaatc gttgggcggg tccagggcga attttgcgac
     1561 aacatgtcga ggctcagcag gagcggccgc ggcgcgccgt aatcatggtc atagctgttt
     1621 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     1681 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1741 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1801 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1861 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1921 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1981 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2041 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2101 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2161 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     2221 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2281 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2341 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2401 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     2461 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2521 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2581 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2641 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2701 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2761 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2821 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2881 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2941 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3001 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3061 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3121 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3181 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3241 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3301 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3361 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3421 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3481 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3541 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3601 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3661 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3721 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     3781 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
