LOCUS       40924_38468        4553 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAT1-EGFP-C1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Tzfira T, Tian GW, Lacroix B, Vyas S, Li J, Leitner-Dagan Y, 
            Krichevsky A, Taylor T, Vainstein A, Citovsky V.
  TITLE     pSAT vectors: a modular series of plasmids for autofluorescent 
            protein tagging and expression of multiple genes in plants
  JOURNAL   Plant Mol. Biol. 57 (4), 503-516 (2005)
  PUBMED    15821977
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Tzfira T, Lacroix B, Citovsky V.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2004) Department of Biochemistry and Cell Biology,
            State University of New York Stony Brook, 100 Nicolls Rd, Stony 
            Brook, NY 11794, USA
REFERENCE   3  (bases 1 to 4553)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4553)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2005"; volume: "57"; issue: "4"; pages: "503-516"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-NOV-2004) Department of Biochemistry and Cell Biology, State 
            University of New York Stony Brook, 100 Nicolls Rd, Stony Brook, NY 
            11794, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        487..1158
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    1168..1298
                     /note="5' UTR from tobacco etch virus; translational
                     enhancer"
     CDS             1301..2017
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    2018..2083
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      2096..2306
                     /label=CaMV 35S terminator
                     /note="CaMV 35S terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(2332..2348)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2356..2372)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2380..2410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2425..2446)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2734..3322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3496..4353)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4354..4458)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccgg cgcgccaccg gtcaacatgt
      421 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag
      481 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc
      541 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca
      601 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga
      661 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
      721 gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat
      781 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc
      841 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga
      901 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga
      961 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa
     1021 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt
     1081 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc
     1141 atttcatttg gagaggacgt cgagagttct caacacaaca tatacaaaac aaacgaatct
     1201 caagcaatca agcattctac ttctattgca gcaatttaaa tcatttcttt taaagcaaaa
     1261 gcaattttct gaaaattttc accatttacg aacgatagcc atggtgagca agggcgagga
     1321 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1381 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1441 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     1501 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     1561 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1621 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1681 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1741 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1801 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1861 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     1921 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1981 cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat ctcgagctca
     2041 agcttcgaat tctgcagtcg acggtaccgc gggcccggga tccacctagt ctagagtccg
     2101 caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt
     2161 gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc
     2221 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct
     2281 aattcctaaa accaaaatcc agtgacgcgg ccgcggcgcg ccgtaatcat ggtcatagct
     2341 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     2401 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     2461 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     2521 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2581 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2641 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2701 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2761 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2821 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2881 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
     2941 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3001 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3061 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3121 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     3181 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3241 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3301 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3361 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3421 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3481 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3541 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3601 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3661 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3721 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3781 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3841 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3901 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     3961 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     4021 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4081 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     4141 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4201 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4261 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4321 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4381 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4441 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     4501 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
