LOCUS       40924_38453        6348 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSAM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Belbahri L, Torche S, Mauch F.
  TITLE     Versatile cloning vectors for Phytophthora transformation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   Belbahri L, Torche S, Mauch F.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-OCT-2007) Laboratory of Applied Genetics, School of 
            Engineering of Lullier, 150 Route de Presinge, Jussy 1254, 
            Switzerland
REFERENCE   3  (bases 1 to 6348)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6348)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-OCT-2007) Laboratory of Applied Genetics, School of Engineering 
            of Lullier, 150 Route de Presinge, Jussy 1254, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     670..686
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(720..736)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             753..1466
                     /label=GFP (S65T)
                     /note="S65T variant of Aequorea victoria green fluorescent 
                     protein (Heim et al., 1995)"
     regulatory      1506..2026
                     /label=3' Bremia lactucae Ham34 terminator
                     /note="3' Bremia lactucae Ham34 terminator"
                     /regulatory_class="terminator"
     regulatory      2034..2617
                     /label=5' Bremia lactucae Hsp70 promoter
                     /note="5' Bremia lactucae Hsp70 promoter"
                     /regulatory_class="promoter"
     CDS             2633..3424
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4160..4178)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4199..4215)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4223..4239)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4247..4277)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4292..4313)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4601..5189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5363..6220)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6221..6325)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg
      721 atccactagt tctagagcgg ccgccaccgc ggatgggcaa gggcgaggaa ctgttcactg
      781 gcgtggtccc aatcctggtg gaactggatg gtgatgtgaa cgggcacaag ttctccgtca
      841 gcggagaggg tgaaggtgat gccacctacg gaaagctcac cctgaagttc atctgcacta
      901 ccggaaagct ccctgttccg tggccaaccc tcgtcaccac tttcacctac ggtgttcagt
      961 gcttctcccg gtacccagat cacatgaagc agcatgactt cttcaagagc gccatgcccg
     1021 aaggctacgt gcaagaaagg actatcttct tcaaggatga cgggaactac aagacacgtg
     1081 ccgaagtcaa gttcgaaggt gataccctgg tgaaccgcat cgagctgaaa ggcatcgatt
     1141 tcaaggaaga tggaaacatc cttggacaca agctggagta caactacaac tcccacaacg
     1201 tatacatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atcaggcaca
     1261 acatcgaaga tggaagcgtg caactggcgg accactacca gcagaacacg cccatcggcg
     1321 atggccctgt cctgctgccg gacaaccatt acctgtccac gcaatctgcc ctctccaagg
     1381 accccaacga gaagagggac cacatggtcc tgctggagtt cgtgacggct gctgggatca
     1441 cgcatggcat ggatgaactc tacaagtgaa gatctgaatt gggtaccggt gcctacttct
     1501 tgtaagccga aggtcgagct gtgtgcactg agtgcgaagt tgcagatttt gtgtctatgc
     1561 atttaataca atttcctttt ttccattttc gttgtttttt tttgtgaaag aacgattgtt
     1621 ttcaaaagaa ggccgtttgt agaacaattt ttttttatac tagcatttgg tattcgagtg
     1681 tgacgaatcg gtgatggcgc tttacaaatg aaatataacg ctgaacaagc gcagtgacag
     1741 ctttatttta taacagagaa taaagaccaa agtagagtag actgatggcg gcttgaaaca
     1801 aaactacgtg acttctaatg caaagaaaac cataccccac gctacttaga attgtttctc
     1861 tttggttgat gaccgctata gtcgtatgga gtgatccctt tttgtcaata tttcgattct
     1921 tgctaaaacc gtgactattt tgaggcaact gtgtgtactt aacatggtag aaatttctat
     1981 gtttaagcca gctgacaatc attctttatt tatcgtaaac caaccaccat atgcgtcaat
     2041 gcaattgact gtgtcctcga ttatttctat tattaatatt atttccatta aaaaagaaat
     2101 ccagttcgtg gcaaaataag acaaaggttt aaataaaaat ttatcgttat taaactcgta
     2161 acttaccttc ttcatggctc aacccaatct gtatatcgac ttatttaatt tggttaacaa
     2221 atcggttttc gtcgcaaata ggggatgaca actaaaatac caatttgtgt ccttgcgaca
     2281 cgtcaacagg tggctgccaa tatctcaggt tttgagcaca caacgctgtg attggctatc
     2341 tgtagatcgc ttcgagaact ttcggaccga catatcagaa cgcagtcatg tccaatccag
     2401 ctaacgattg gtcgaaaccg cacttcatcg cttcatttca accaattccc atctactaaa
     2461 acgagcgtcg gaatagtctc gaacgtaacc ccacgtagtg ggtggcagcg cccctcgtat
     2521 ctttcaatcg ccactcgagt ctcaagtttg ctcactttga aattttccat ctggactaca
     2581 catcaaatct aactcaactt ttatcaattg aaacacaagg gccccgtttc gcatgattga
     2641 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     2701 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     2761 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     2821 gccagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     2881 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     2941 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     3001 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     3061 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     3121 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     3181 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     3241 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     3301 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     3361 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     3421 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     3481 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     3541 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     3601 ccggatcccc ggggtcgact agaatactta gcaattggtt agtttgaaaa ttgtcaaagc
     3661 agagtttttt cgacaataaa atttattttc taaatatttg atttagcgtt gacttaattt
     3721 gttgattcgg ttgtcatctt ttgtgaagat gcccgagagt ctagtaaatc tcaagcgaaa
     3781 catagtgaga aggtcactat ggctggtttg catgagtctg ggcctattgt gcttacgccg
     3841 ccttagtaac aaacatgcca aaagaaggaa agctttcacc agctttcaag tttaatcaat
     3901 ctttgagagt cacaattgca agaataaatg aatttttaca acacagttcg atgcactgtg
     3961 tttcgtcttg atgaaaattg gacggcaaat cgagtatctt aaacaaatct cggcataggc
     4021 tgtccatttt gtggtcgcct ctttgttggt agtttgcagt agtttcatta gtagaagatc
     4081 tgcatcctca atctgctaaa cgtcttgagc agctatgaga cgatccgggg aattggggcc
     4141 cgagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
     4201 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     4261 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     4321 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     4381 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     4441 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     4501 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     4561 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     4621 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     4681 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     4741 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     4801 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     4861 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4921 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4981 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5041 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5101 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     5161 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     5221 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     5281 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     5341 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     5401 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     5461 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     5521 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     5581 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     5641 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     5701 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     5761 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     5821 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     5881 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     5941 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6001 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6061 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6121 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     6181 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     6241 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     6301 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
