LOCUS       40924_38413        5190 bp DNA     circular SYN 18-DEC-2018
DEFINITION  SEAP expression vector pSA928, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Auslander S, Stucheli P, Rehm C, Auslander D, Hartig JS, Fussenegger
            M.
  TITLE     A general design strategy for protein-responsive riboswitches in 
            mammalian cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Auslander S, Stucheli P, Rehm C, Auslander D, Hartig JS, Fussenegger
            M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-AUG-2014) Department of Biosystems Science and 
            Engineering, D-BSSE, ETH Zurich, Mattenstrasse 26, Basel, BS 4058, 
            Switzerland
REFERENCE   3  (bases 1 to 5190)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5190)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-AUG-2014) Department of Biosystems Science and Engineering, 
            D-BSSE, ETH Zurich, Mattenstrasse 26, Basel, BS 4058, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..244
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             266..1774
                     /codon_start=1
                     /label=SEAP
                     /note="secreted alkaline phosphatase from human placenta"
                     /translation="PTMLLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKL
                     QPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNV
                     DKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVG
                     VVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGG
                     RKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSV
                     THLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHE
                     SRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPG
                     KARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAV
                     FARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     misc_feature    1858..1932
                     /label=HHR Env14_U1hpII
                     /note="HHR Env14_U1hpII"
     polyA_signal    complement(1947..2028)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2693..3281)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3455..4312)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4313..4417)
                     /label=AmpR promoter
     rep_origin      4444..4899
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5030..5078
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5092..5183
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
       61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
      181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      241 caaaaagctt cgaatcgcga attcgcccac catgctgctg ctgctgctgc tgctgggcct
      301 gaggctacag ctctccctgg gcatcatccc agttgaggag gagaacccgg acttctggaa
      361 ccgcgaggca gccgaggccc tgggtgccgc caagaagctg cagcctgcac agacagccgc
      421 caagaacctc atcatcttcc tgggcgatgg gatgggggtg tctacggtga cagctgccag
      481 gatcctaaaa gggcagaaga aggacaaact ggggcctgag atacccctgg ccatggaccg
      541 cttcccatat gtggctctgt ccaagacata caatgtagac aaacatgtgc cagacagtgg
      601 agccacagcc acggcctacc tgtgcggggt caagggcaac ttccagacca ttggcttgag
      661 tgcagccgcc cgctttaacc agtgcaacac gacacgcggc aacgaggtca tctccgtgat
      721 gaatcgggcc aagaaagcag ggaagtcagt gggagtggta accaccacac gagtgcagca
      781 cgcctcgcca gccggcacct acgcccacac ggtgaaccgc aactggtact cggacgccga
      841 cgtgcctgcc tcggcccgcc aggaggggtg ccaggacatc gctacgcagc tcatctccaa
      901 catggacatt gacgtgatcc taggtggagg ccgaaagtac atgtttcgca tgggaacccc
      961 agaccctgag tacccagatg actacagcca aggtgggacc aggctggacg ggaagaatct
     1021 ggtgcaggaa tggctggcga agcgccaggg tgcccggtat gtgtggaacc gcactgagct
     1081 catgcaggct tccctggacc cgtctgtgac ccatctcatg ggtctctttg agcctggaga
     1141 catgaaatac gagatccacc gagactccac actggacccc tccctgatgg agatgacaga
     1201 ggctgccctg cgcctgctga gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg
     1261 tcgcatcgac catggtcatc atgaaagcag ggcttaccgg gcactgactg agacgatcat
     1321 gttcgacgac gccattgaga gggcgggcca gctcaccagc gaggaggaca cgctgagcct
     1381 cgtcactgcc gaccactccc acgtcttctc cttcggaggc taccccctgc gagggagctc
     1441 catcttcggg ctggcccctg gcaaggcccg ggacaggaag gcctacacgg tcctcctata
     1501 cggaaacggt ccaggctatg tgctcaagga cggcgcccgg ccggatgtta ccgagagcga
     1561 gagcgggagc cccgagtatc ggcagcagtc agcagtgccc ctggacgaag agacccacgc
     1621 aggcgaggac gtggcggtgt tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca
     1681 ggagcagacc ttcatagcgc acgtcatggc cttcgccgcc tgcctggagc cctacaccgc
     1741 ctgcgacctg gcgccccccg ccggcaccac cgacgccgcg cacccgggtt actctagagt
     1801 cggggcggcc ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaagtt
     1861 taaaccctcc tcgtccacac attgcacaag gtgtgctgac gagtcgtaaa cgacgaaacg
     1921 aggaggacta gtaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     1981 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2041 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     2101 aacctctaca aatgtggtaa aatcgataag gatctgaacg atggagcgga gaatgggcgg
     2161 aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact
     2221 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
     2281 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
     2341 actttccaca ccctaactga cacacattcc acagcggatc cgtcgaccga tgcccttgag
     2401 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     2461 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt
     2521 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2581 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2641 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2701 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2761 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2821 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2881 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2941 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3001 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3061 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3121 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3181 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3241 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3301 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3361 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3421 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3481 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3541 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3601 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3661 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3721 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3781 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3841 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     3901 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     3961 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4021 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4081 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4141 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4201 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4261 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4321 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4381 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     4441 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4501 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4561 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4621 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4681 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4741 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4801 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4861 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ccattcgcca ttcaggctgc
     4921 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac
     4981 catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc
     5041 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
     5101 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
     5161 tgcaggtgcc agaacatttc tctatcgata
//
