LOCUS       40924_38378        8570 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Signal sequence gene trap vector pSA-TMbgyg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8570)
  AUTHORS   Shirasawa S, Yoshimi M, Kamochi H, Muneta T, Kimura JH, Hook M, 
            Shinomura T.
  TITLE     Gene trap screening for cell surface and extracellular matrix 
            molecules produced by chondrocytes
  JOURNAL   J. Biochem. 137 (1), 79-85 (2005)
  PUBMED    15713886
REFERENCE   2  (bases 1 to 8570)
  AUTHORS   Shinomura T, Shirasawa S.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2004) Tamayuki Shinomura, Tokyo Medical and Dental
            University, Tissue Regeneration, Dept. of Hard Tissue Engineering; 
            1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan 
            (E-mail:t.shinomura.trg@tmd.ac.jp, Tel:81-3-5803-5450, 
            Fax:81-3-5803-0187)
REFERENCE   3  (bases 1 to 8570)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8570)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biochem."; date: "2005"; volume: "137"; issue: "1"; pages: "79-85"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-JUL-2004) Tamayuki Shinomura, Tokyo Medical and Dental 
            University, Tissue Regeneration, Dept. of Hard Tissue Engineering"; 
            volume: " 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan 
            (E-mail:t.shinomura.trg@tmd.ac.jp, Tel:81-3-5803-5450, Fax"; pages: 
            "81-3-5803-0187"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    17..120
                     /label=splicing acceptor
                     /note="splicing acceptor"
     misc_feature    144..804
                     /gene="TM(CD4)"
                     /product="transmembrane domain of rat CD4"
     gene            144..804
                     /gene="TM(CD4)"
                     /label=TM(CD4)
     CDS             814..3855
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             3871..4905
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4950..4998
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      5189..5408
                     /regulatory_class="terminator"
     promoter        complement(5439..5457)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5475..5491)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5499..5515)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5523..5553)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5568..5589)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5877..6465)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6639..7496)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7497..7601)
                     /label=AmpR promoter
     rep_origin      7935..8390
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     8531..8547
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8554..8570
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattc gagctcaagc ttgaggtgtg gcaggcttga gatctggcca tacacttgag
       61 tgacaatgac atccactttg cctttctctc cacaggtgtc cactcccagg tccaactgca
      121 gtcgacggta ccgcgggccc catgggagag ttgagatgga aggcagagaa ggctccttct
      181 tcccagtcct ggatcacctt ctccctaaag aaccaaaagg tgtctgtgca gaagtctacc
      241 agcaacccca agttccagct gtccgaaacg ctcccactca cccttcagat accccaggtc
      301 tcccttcagt ttgctggttc tggcaacctg accctgactc tggacagagg gatactgtat
      361 caggaagtga acctggtggt gatgaaagtg actcagcccg acagcaacac tttgacctgt
      421 gaggtgatgg gacctacctc acccaagatg agactgatct tgaagcagga gaatcaggag
      481 gccagggtct ccaggcagga gaaagtgatt caagtgcagg cccctgaagc aggggtgtgg
      541 caatgtctac tgagtgaagg tgaagaggtc aagatggact ccaagatcca ggttttatcc
      601 aaagggttga accagacaat gttcctggct gtcgtgctgg ggagcgcctt cagctttctg
      661 gttttcacgg ggctctgcat cctattctgt gtcaggtgcc ggcaccaaca gcgccaggca
      721 gcacggatgt ctcagatcaa gaggcttctc agtgagaaga agacttgcca gtgctcccac
      781 cggatgcaga aaagccacaa tctcatggat cccgtcgttt tacaacgtcg tgactgggaa
      841 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      901 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      961 tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct ggagtgcgat
     1021 cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg
     1081 cccatctaca ccaacgtaac ctatcccatt acggtcaatc cgccgtttgt tcccacggag
     1141 aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc
     1201 cagacgcgaa ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc
     1261 tgggtcggtt acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta
     1321 cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgtt ggagtgacgg cagttatctg
     1381 gaagatcagg atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcataaa
     1441 ccgactacac aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc
     1501 gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc gtgactacct acgggtaaca
     1561 gtttctttat ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa
     1621 attatcgatg agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac
     1681 ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg cggtggttga actgcacacc
     1741 gccgacggca cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt
     1801 gaaaatggtc tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac
     1861 gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg
     1921 ctgatgaagc agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg
     1981 tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc
     2041 cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc gctggctacc ggcgatgagc
     2101 gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg
     2161 ctggggaatg aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct
     2221 gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag ccgacaccac ggccaccgat
     2281 attatttgcc cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa
     2341 tggtccatca aaaaatggct ttcgctacct ggagagacgc gcccgctgat cctttgcgaa
     2401 tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta aatactggca ggcgtttcgt
     2461 cagtatcccc gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa
     2521 tatgatgaaa acggcaaccc gtggtcggct tacggcggtg attttggcga tacgccgaac
     2581 gatcgccagt tctgtatgaa cggtctggtc tttgccgacc gcacgccgca tccagcgctg
     2641 acggaagcaa aacaccagca gcagtttttc cagttccgtt tatccgggca aaccatcgaa
     2701 gtgaccagcg aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg
     2761 ctggatggta agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa
     2821 cagttgattg aactgcctga actaccgcag ccggagagcg ccgggcaact ctggctcaca
     2881 gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg
     2941 cagcagtggc gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc
     3001 ccgcatctga ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa
     3061 tttaaccgcc agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg
     3121 acgccgctgc gcgatcagtt cacccgtgca ccgctggata acgacattgg cgtaagtgaa
     3181 gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag
     3241 gccgaagcag cgttgttgca gtgcacggca gatacacttg ctgatgcggt gctgattacg
     3301 accgctcacg cgtggcagca tcaggggaaa accttattta tcagccggaa aacctaccgg
     3361 attgatggta gtggtcaaat ggcgattacc gttgatgttg aagtggcgag cgatacaccg
     3421 catccggcgc ggattggcct gaactgccag ctggcgcagg tagcagagcg ggtaaactgg
     3481 ctcggattag ggccgcaaga aaactatccc gaccgcctta ctgccgcctg ttttgaccgc
     3541 tgggatctgc cattgtcaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg
     3601 cgctgcggga cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc
     3661 aacatcagcc gctacagtca acagcaactg atggaaacca gccatcgcca tctgctgcac
     3721 gcggaagaag gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac
     3781 tcctggagcc cgtcagtatc ggcggaattc cagctgagcg ccggtcgcta ccattaccag
     3841 ttggtctggt gtcaggggat cctcgccacc atgaaaaagc ctgaactcac cgcgacgtct
     3901 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     3961 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     4021 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     4081 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     4141 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     4201 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     4261 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     4321 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     4381 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     4441 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     4501 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     4561 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     4621 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     4681 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     4741 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     4801 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     4861 cgacgcccca gcactcgtcc ggatcgggag atgggggagg ctaactgaaa cacggaagga
     4921 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     4981 gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata
     5041 ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc
     5101 ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca
     5161 tagccactgg ccccgtgggt tagggacggg gtcccccatg gggaatggtt tatggttcgt
     5221 gggggttatt attttgggcg ttgcgtgggg tcaggtccac gactggactg agcagacaga
     5281 cccatggttt ttggatggcc tgggcatgga ccgcatgtac tggcgcgaca cgaacaccgg
     5341 gcgtctgtgg ctgccaaaca cccccgaccc ccaaaaacca ccgcgcggat ttctggcgtg
     5401 ccaagctagt cgacctgcag gcatgcaagc ttgagtattc tatagtgtca cctaaatagc
     5461 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     5521 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     5581 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     5641 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     5701 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     5761 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     5821 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5881 gataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5941 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6001 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6061 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6121 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6181 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6241 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6301 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6361 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6421 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6481 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6541 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6601 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6661 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6721 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6781 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6841 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6901 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttggcattgc tacaggcatc
     6961 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7021 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7081 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7141 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7201 tcattctgag aataccgcgc ccggcgaccg agttgctctt gcccggcgtc aatacgggat
     7261 aatagtgtat gacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7321 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7381 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7441 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7501 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7561 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7621 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     7681 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     7741 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     7801 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     7861 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     7921 taccgcatca ggcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7981 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     8041 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     8101 agggttccga tttagagctt tacggcacct cgaccgcaaa aaacttgatt tgggtgatgg
     8161 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     8221 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     8281 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     8341 ttaacaaata tttaacgcga attttaacaa aatattaacg tttacaattt ccattcgcca
     8401 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     8461 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     8521 tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
//
