LOCUS       40924_38373        3052 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pS19mob2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Tools for genetic engineering in the amino acid-producing bacterium 
            Corynebacterium glutamicum
  JOURNAL   J. Biotechnol. 104 (1-3), 287-299 (2003)
  PUBMED    12948646
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2003) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   3  (bases 1 to 3052)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3052)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol. 104 (1-3), 287-299 (2003)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2003) Department of Genetics, University of Bielefeld, 
            Universitaetsstrasse 25, Bielefeld D-33615, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..57)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(58..74)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             821..1588
                     /codon_start=1
                     /gene="aad9"
                     /product="Aad9"
                     /label=aad9
                     /note="spectinomycin adenylyltransferase"
                     /protein_id="AAO66597.1"
                     /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS
                     DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ
                     EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR
                     RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI
                     LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL"
     gene            821..1588
                     /gene="aad9"
                     /label=aad9
     oriT            complement(1730..1838)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2050..2638
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2926..2947
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2962..2992
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3000..3016
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3024..3040
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcact
       61 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      121 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      181 ttcccaacag ttgcgcagcc tgaatggcga atggcgcgat aagctagctt cacgctgccg
      241 caagcactca gggcgcaagg gctgctaaag gaagcggaac acgtagaaag ccagtccgca
      301 gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc
      361 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc
      421 ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg
      481 gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg
      541 atcaagatct atcgattttc gttcgtgaat acatgttata ataactataa ctaataacgt
      601 aacgtgactg gcaagagata tttttaaaac aatgaatagg tttacactta ctttagtttt
      661 atggaaatga aagatcatat catatataat ctagaataaa attaactaaa ataattatta
      721 tctagataaa aaatttagaa gccaatgaaa tctataaata aactaaatta agtttattta
      781 attaacaact atggatataa aataggtact aatcaaaata gtgaggagga tatatttgaa
      841 tacatacgaa caaattaata aagtgaaaaa aatacttcgg aaacatttaa aaaataacct
      901 tattggtact tacatgtttg gatcaggagt tgagagtgga ctaaaaccaa atagtgatct
      961 tgacttttta gtcgtcgtat ctgaaccatt gacagatcaa agtaaagaaa tacttataca
     1021 aaaaattaga cctatttcaa aaaaaatagg agataaaagc aacttacgat atattgaatt
     1081 aacaattatt attcagcaag aaatggtacc gtggaatcat cctcccaaac aagaatttat
     1141 ttatggagaa tggttacaag agctttatga acaaggatac attcctcaga aggaattaaa
     1201 ttcagattta accataatgc tttaccaagc aaaacgaaaa aataaaagaa tatacggaaa
     1261 ttatgactta gaggaattac tacctgatat tccattttct gatgtgagaa gagccattat
     1321 ggattcgtca gaggaattaa tagataatta tcaggatgat gaaaccaact ctatattaac
     1381 tttatgccgt atgattttaa ctatggacac gggtaaaatc ataccaaaag atattgcggg
     1441 aaatgcagtg gctgaatctt ctccattaga acatagggag agaattttgt tagcagttcg
     1501 tagttatctt ggagagaata ttgaatggac taatgaaaat gtaaatttaa ctataaacta
     1561 tttaaataac agattaaaaa aattataaaa aaattgaaaa aatggtggaa acactttttt
     1621 caattttttt gttttattat ttaatatttg ggaaatattc attctaattg gtaatcagat
     1681 tttagaaaac aataaaccct tgcatatgat atcgatgtac agatctgatc ggccagcctc
     1741 gcagagcagg attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac
     1801 acccgctcgc gggtgggcct acttcaccta tcctgccccg ctgacgccgt tggatacacc
     1861 aaggaaagtc tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat
     1921 ataccgaaaa aatcgctata atgaccccga agcagggtta tgcagcggaa aagccatgac
     1981 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2041 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2101 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2161 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     2221 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2281 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2341 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2401 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct
     2461 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2521 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2581 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2641 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2701 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2761 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2821 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     2881 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     2941 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     3001 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg cc
//
