LOCUS       40924_38368        3051 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pS18mob2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Tools for genetic engineering in the amino acid-producing bacterium 
            Corynebacterium glutamicum
  JOURNAL   J. Biotechnol. 104 (1-3), 287-299 (2003)
  PUBMED    12948646
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2003) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   3  (bases 1 to 3051)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3051)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol. 104 (1-3), 287-299 (2003)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2003) Department of Genetics, University of Bielefeld, 
            Universitaetsstrasse 25, Bielefeld D-33615, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3051
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(61..77)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             824..1591
                     /codon_start=1
                     /gene="aad9"
                     /product="Aad9"
                     /label=aad9
                     /note="spectinomycin adenylyltransferase"
                     /protein_id="AAO66596.1"
                     /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS
                     DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ
                     EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR
                     RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI
                     LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL"
     gene            824..1591
                     /gene="aad9"
                     /label=aad9
     oriT            complement(1733..1841)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2053..2641
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2929..2950
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2965..2995
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3003..3019
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3027..3043
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
       61 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      121 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      181 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc gataagctag cttcacgctg
      241 ccgcaagcac tcagggcgca agggctgcta aaggaagcgg aacacgtaga aagccagtcc
      301 gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa
      361 cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg
      421 ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt
      481 tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag
      541 gggatcaaga tctatcgatt ttcgttcgtg aatacatgtt ataataacta taactaataa
      601 cgtaacgtga ctggcaagag atatttttaa aacaatgaat aggtttacac ttactttagt
      661 tttatggaaa tgaaagatca tatcatatat aatctagaat aaaattaact aaaataatta
      721 ttatctagat aaaaaattta gaagccaatg aaatctataa ataaactaaa ttaagtttat
      781 ttaattaaca actatggata taaaataggt actaatcaaa atagtgagga ggatatattt
      841 gaatacatac gaacaaatta ataaagtgaa aaaaatactt cggaaacatt taaaaaataa
      901 ccttattggt acttacatgt ttggatcagg agttgagagt ggactaaaac caaatagtga
      961 tcttgacttt ttagtcgtcg tatctgaacc attgacagat caaagtaaag aaatacttat
     1021 acaaaaaatt agacctattt caaaaaaaat aggagataaa agcaacttac gatatattga
     1081 attaacaatt attattcagc aagaaatggt accgtggaat catcctccca aacaagaatt
     1141 tatttatgga gaatggttac aagagcttta tgaacaagga tacattcctc agaaggaatt
     1201 aaattcagat ttaaccataa tgctttacca agcaaaacga aaaaataaaa gaatatacgg
     1261 aaattatgac ttagaggaat tactacctga tattccattt tctgatgtga gaagagccat
     1321 tatggattcg tcagaggaat taatagataa ttatcaggat gatgaaacca actctatatt
     1381 aactttatgc cgtatgattt taactatgga cacgggtaaa atcataccaa aagatattgc
     1441 gggaaatgca gtggctgaat cttctccatt agaacatagg gagagaattt tgttagcagt
     1501 tcgtagttat cttggagaga atattgaatg gactaatgaa aatgtaaatt taactataaa
     1561 ctatttaaat aacagattaa aaaaattata aaaaaattga aaaaatggtg gaaacacttt
     1621 tttcaatttt tttgttttat tatttaatat ttgggaaata ttcattctaa ttggtaatca
     1681 gattttagaa aacaataaac ccttgcatat gatatcgatg tacagatctg atcggccagc
     1741 ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg
     1801 aacacccgct cgcgggtggg cctacttcac ctatcctgcc ccgctgacgc cgttggatac
     1861 accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg
     1921 gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagccat
     1981 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2041 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2101 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2161 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2221 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2281 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2341 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2401 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     2461 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2521 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2581 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2641 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2701 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2761 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     2821 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     2881 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     2941 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     3001 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta c
//
