LOCUS       62056_19125        5311 bp DNA     circular SYN 16-AUG-2021
DEFINITION  Cloning vector pS+TAN__SAYQR, complete sequence.
ACCESSION   MZ198788
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Groseclose TM, Hersey AN, Huang BD, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (13-MAY-2021) Chemical "
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        83..160
                     /label=lacI promoter
     CDS             161..1207
                     /codon_start=1
                     /transl_table=11
                     /product="S+TAN"
                     /label=S+TAN
                     /protein_id="QYJ58373.1"
                     /translation="MKPVTLYDVAEYAGVSTATVSNVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAAQLAGKQSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNL
                     SGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGAGTPD
                     MPGGEAAMVKLLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIAR
                     YTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAI
                     TNSTNQAM"
     protein_bind    1337..1358
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1852..2396)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     protein_bind    complement(2587..2608)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2738..3784)
                     /codon_start=1
                     /transl_table=11
                     /product="SAYQR"
                     /label=SAYQR
                     /protein_id="QYJ58374.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAAQLAGKQSDTIGVVVMDVTDAFFGALVKAVDQVAQQHQKYVAIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNL
                     SGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGAGTPD
                     MPGGEAALVKLLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIAR
                     YTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAI
                     TNSTNQAM"
     promoter        complement(3785..3862)
                     /label=lacI promoter
     CDS             complement(4461..5117)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(5118..5220)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
       61 aagggagagc gtcgagatcc cggacaccat cgaatggcgc aaaacctttc gcggtatggc
      121 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata
      181 cgatgtcgca gagtatgccg gtgtctctac cgcgaccgtt tccaacgtgg tgaaccaggc
      241 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctcaatta
      301 cattcccaac cgcgtggcag cccaactggc gggcaaacag agcgacacca ttggcgtggt
      361 ggtgatggac gtttctgatg cgtttttcgg cgcgctggta aaagcggtgg atctggtcgc
      421 tcagcagcat cagaaatacg tgctaatcgg caatagctat catgaagcgg aaaaagagcg
      481 tcacgccatt gaggtgttaa ttcgccagcg ttgtaatgcg ttgattgttc actcaaaagc
      541 attgagtgac gatgaactgg cgcaatttat ggataacatt cccggtatgg tgttaatcaa
      601 ccgcgttgtg ccggggtacg cccatcgttg cgtttgcctg gataatctca gcggtgcccg
      661 aatggcgacg cgcatgttgc tgaataacgg tcatcaacgt attggttatc tttcttccag
      721 ccacggcatt gaagatgacg ccatgcgtaa agcaggctgg atgagtgcgt tgaaagagca
      781 ggatattatt ccgccggaaa gctggattgg cgctggtacg ccggacatgc cgggcggtga
      841 ggcggcgatg gttaaactgc tggggcgcaa tctacaactt accgctgtat ttgcttataa
      901 cgacaatatg gccgctggcg cactgacagc attaaaagat aatggcattg cgattccgtt
      961 acatctctca atcatcgggt tcgatgatat tcccatcgcc cgttacaccg acccgcaatt
     1021 aacgaccgtg cgttatccca ttgcttcaat ggcgaaatta gccaccgaac tggccttgca
     1081 gggggcagca ggcaatattg atcctcgtgc cagccactgt tttatgccga cgttagtgcg
     1141 tcgccattct gtcgcaacac gccagaatgc ggcggcgatc actaactcaa caaatcaggc
     1201 gatgtaactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     1261 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     1321 gtgagcgcaa cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga
     1381 tgcccttgag agccttcaac ccagtcagct ccttccgcag gtatttattc ggcgcaaagt
     1441 gcgtcgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc
     1501 agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa
     1561 cggcaaaagc accgccggac atcagcgcta gcggagtgta tactggctta ctatgttggc
     1621 actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc
     1681 gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct
     1741 cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag
     1801 atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag
     1861 gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc
     1921 gacaggacta taaagatacc aggcgtttcc cctggcggct ccctcgtgcg ctctcctgtt
     1981 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
     2041 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     2101 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
     2161 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga
     2221 agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa
     2281 gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca
     2341 aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag
     2401 atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc tcttcaaatg
     2461 tagcacctga agtcagcccc atacgatata agttgtaatt ctcatgttag tcatgccccg
     2521 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg
     2581 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc
     2641 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2701 gcgtattggg cgccagggtg gtttttcttt tcaccagtta catcgcctga tttgttgagt
     2761 tagtgatcgc cgccgcattc tggcgtgttg cgacagaatg gcgacgcact aacgtcggca
     2821 taaaacagtg gctggcacga ggatcaatat tgcctgctgc cccctgcaag gccagttcgg
     2881 tggctaattt cgccattgaa gcaatgggat aacgcacggt cgttaattgc gggtcggtgt
     2941 aacgggcgat gggaatatca tcgaacccga tgattgagag atgtaacgga atcgcaatgc
     3001 cattatcttt taatgctgtc agtgcgccag cggccatatt gtcgttataa gcaaatacag
     3061 cggtaagttg tagattgcgc cccagcagtt taaccaacgc cgcctcaccg cccggcatgt
     3121 ccggcgtacc agcgccaatc cagctttccg gcggaataat atcctgctct ttcaacgcac
     3181 tcatccagcc tgctttacgc atggcgtcat cttcaatgcc gtggctggaa gaaagataac
     3241 caatacgttg atgaccgtta ttcagcaaca tgcgcgtcgc cattcgggca ccgctgagat
     3301 tatccagaca aacgcaacga tgggcgtacc ccggcacaac gcggttgatt aacaccatac
     3361 cgggaatgtt atccataaat tgcgccagtt catcgtcact caatgctttt gagtgaacaa
     3421 tcaacgcatt acaacgctgg cgaattaaca cctcaatggc gtgacgctct ttttccgctt
     3481 catgatagct attgccgatt gccacgtatt tctgatgctg ctgagcgacc tgatccaccg
     3541 cttttaccag cgcgccgaaa aacgcatcag taacgtccat caccaccacg cctatggtgt
     3601 ccgactgttt gcccgccagt tgggctgcca cgcggttggg aatgtaattg agctccgcca
     3661 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
     3721 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt
     3781 tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
     3841 ttttgcgcca ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt
     3901 aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgtc
     3961 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     4021 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgccctgc
     4081 accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca
     4141 tctgtattaa cgaagcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca
     4201 tatcgtcaat tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga
     4261 agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag
     4321 cggctattta acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct
     4381 gccattcatc cgcttattat cacttattca ggcgtagcac caggcgttta agggcaccaa
     4441 taactgcctt aaaaaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca
     4501 ttaagcattc tgccgacatg gaagccatca cagacggcat gatgaacctg aatcgccagc
     4561 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag
     4621 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct
     4681 gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa
     4741 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc
     4801 cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta
     4861 tcccatatca ccagctcacc gtctttcatt gccatacgga attccggatg agcattcatc
     4921 aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc
     4981 tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac
     5041 tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca
     5101 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat
     5161 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca
     5221 acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg gacaccagga
     5281 tttatttatt ctgcgaagtg atcttccgtc a
//
