LOCUS       40924_38318        7039 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Chloroplast transformation vector pRvdB1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Van Den Bekerom RJM., Hogg B, McCabe MS.
  TITLE     Transformation vector with loxp-sites and aadA for targeting the 
            trnA and trnI region of the tobacco chloroplast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7039)
  AUTHORS   Van Den Bekerom RJM., Hogg B, McCabe MS.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2005) Biology Department, National University of 
            Ireland, Maynooth, Maynooth, County Kildare, Ireland
REFERENCE   3  (bases 1 to 7039)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7039)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2005) Biology Department, National University of Ireland, 
            Maynooth, Maynooth, County Kildare, Ireland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            46..827
                     /pseudo
                     /gene="trnA"
                     /label=trnA
     promoter        complement(847..865)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(872..888)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1029..1457
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             1801..2592
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             2613..3470
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3644..4232
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4520..4541
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4556..4586
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4594..4610
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4618..4634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4652..4670
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     gene            4750..5528
                     /pseudo
                     /gene="trnI"
                     /label=trnI
     protein_bind    5545..5578
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      5626..5759
                     /label=Prrn promoter in tobacco plastome
                     /note="Prrn promoter in tobacco plastome"
                     /regulatory_class="promoter"
     CDS             5760..6548
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     3'UTR           6564..6956
                     /label=PsbA-3'UTR
                     /note="PsbA-3'UTR"
     misc_feature    6998..7033
                     /label=loxP site
                     /note="loxP site"
     protein_bind    complement(6999..7032)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
ORIGIN
        1 gtttaaacct gcaggccggc catttaaatg cggccgcggc gcgccgggga tatagctcag
       61 ttggtagagc tccgctcttg caattgggtc gttgcgatta cgggttggat gtctaattgt
      121 ccaggcggta atgatagtat cttgtacctg aaccggtggc tcactttttc taagtaatgg
      181 ggaagaggac cgaaacgtgc cactgaaaga ctctactgag acaaagatgg gctgtcaaga
      241 acgtagagga ggtaggatgg gcagttggtc agatctagta tggatcgtac atggacggta
      301 gttggagtcg gcggctctcc cagggttccc tcatctgaga tctctgggga agaggatcaa
      361 gttggccctt gcgaacagct tgatgcacta tctcccttca accctttgag cgaaatgcgg
      421 caaaagaaaa ggaaggaaaa tccatggacc gaccccatca tctccacccc gtaggaacta
      481 cgagatcacc ccaaggacgc cttcggcatc caggggtcac ggaccgacca tagaaccctg
      541 ttcaataagt ggaacgcatt agctgtccgc tctcaggttg ggcagtcagg gtcggagaag
      601 ggcaatgact cattcttagt tagaatggga ttccaactca gcaccttttg agtgagattt
      661 tgagaagagt tgctctttgg agagcacagt acgatgaaag ttgtaagctg tgttcggggg
      721 ggagttattg tctatcgttg gcctctatgg tagaatcagt cgggggacct gagaggcggt
      781 ggtttaccct gcggcggatg tcagcggttc gagtccgctt atctccatct agagggccca
      841 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
      901 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      961 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     1021 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     1081 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     1141 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     1201 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     1261 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     1321 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     1381 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     1441 aaaatttaac gcgaatttta acaaaattca gggcgcaagg gctgctaaag gaagcggaac
     1501 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct
     1561 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca
     1621 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
     1681 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca
     1741 aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatgagg atcgtttcgc
     1801 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     1861 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     1921 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     1981 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     2041 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     2101 gatctcctgt catcccacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     2161 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     2221 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     2281 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     2341 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     2401 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     2461 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     2521 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     2581 gacgagttct tctgaattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     2641 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2701 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2761 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2821 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     2881 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2941 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     3001 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     3061 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3121 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     3181 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     3241 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     3301 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3361 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3421 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3481 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3541 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3601 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3661 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3721 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3781 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3841 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3901 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3961 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4021 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4081 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4141 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     4201 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     4261 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     4321 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     4381 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     4441 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     4501 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     4561 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     4621 gaaacagcta tgaccatgat tacgccaagc tatttaggtg acactataga atactcaagc
     4681 tatgcatcaa gcttggtacc gagctcggat ccactagtaa cggccgccag tgtgctggaa
     4741 ttcgcccttg ggctattagc tcagtggtag agcgcgcccc tgataattgc gtcgttgtgc
     4801 ctgggctgtg agggctctca gccacatgga tagttcaatg tgctcatcgg cgcctgaccc
     4861 tgagatgtgg atcatccaag gcacattagc atggcgtact cctcctgttc gaaccggggt
     4921 ttgaaaccaa actcctcctc aggaggatag atggggcgat tcgggtgaga tccaatgtag
     4981 atccaacttt cgattcactc gtgggatccg ggcggtccgg gggggaccac cacggctcct
     5041 ctcttctcga gaatccatac atcccttatc agtgtatgga cagctatctc tcgagcacag
     5101 gtttagcaat gggaaaataa aatggagcac ctaacaacgc atcttcacag accaagaact
     5161 acgagatcgc ccctttcatt ctggggtgac ggagggatcg taccattcga gccgtttttt
     5221 tcttgactcg aaatgggagc aggtttgaaa aaggatctta gagtgtctag ggttgggcca
     5281 ggagggtctc ttaacgcctt cttttttctt ctcatcggag ttatttcaca aagacttgcc
     5341 agggtaagga agaagggggg aacaagcaca cttggagagc gcagtacaac ggagagttgt
     5401 atgctgcgtt cgggaaggat gaatcgctcc cgaaaaggaa tctattgatt ctctcccaat
     5461 tggttggacc gtaggtgcga tgatttactt cacgggcgag gtctctggtt caagtccagg
     5521 atggcccatt aattaagtct aggtataact tcgtataatg tatgctatac gaagttatta
     5581 attaggatct agtcgacaga tcccgaattg ggatcggtac ccaaagctcc cccgccgtcg
     5641 ttcaatgaga atggataaga ggctcgtggg attgacgtga gggggcaggg atggctatat
     5701 ttctgggagc gaactccggg cgaatacgaa gcgcttggat acgttgtagg gagggattta
     5761 tggcagaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     5821 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     5881 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     5941 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     6001 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     6061 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     6121 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     6181 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     6241 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     6301 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     6361 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     6421 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     6481 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag
     6541 tcggcaaata atgtctagag cgatcctggc ctagtctata ggaggttttg aaaagaaagg
     6601 agcaataatc attttcttgt tctatcaaga gggtgctatt gctcctttct ttttttcttt
     6661 ttatttattt actagtattt tacttacata gacttttttg tttacattat agaaaaagaa
     6721 ggagaggtta ttttcttgca tttattcatg attgagtatt ctattttgat tttgtatttg
     6781 tttaaaattg tagaaataga acttgtttct cttcttgcta atgttactat atctttttga
     6841 tttttttttt ccaaaaaaaa aatcaaattt tgacttcttc ttatctctta tctttgaata
     6901 tctcttatct ttgaaataat aatatcattg aaataagaaa gaagagctat attcgacctg
     6961 caggcatgca agctatccca attcgggatc ttaagcttat aacttcgtat aatgtatgct
     7021 atacgaagtt attgaattc
//
