LOCUS       62056_18850        7823 bp DNA     circular SYN 12-OCT-2021
DEFINITION  Binary vector pRiBIN1 DNA, complete sequence.
ACCESSION   LC652749
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Banno H.
  TITLE     A binary vector for transient expression in plant cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7823)
  AUTHORS   Banno H.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2021) Contact:Hiroharu Banno Chubu University, 
            College of Bioscience and Biotechnology; 1200 Matsumoto-cho, 
            Kasugai, Aichi 487-8501, Japan URL 
            :https://www3.chubu.ac.jp/biology/
REFERENCE   3  (bases 1 to 7823)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-OCT-2021) Contact:Hiroharu Banno Chubu University, College of 
            Bioscience and Biotechnology; 1200 Matsumoto-cho, Kasugai, Aichi 
            487-8501, Japan URL :https://www3.chubu.ac.jp/biology/"
FEATURES             Location/Qualifiers
     source          1..7823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      56..644
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1116..1137
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1152..1182
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1190..1206
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1214..1230
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1245..5434
                     /label=Ri plasmid replication origin
                     /note="Ri plasmid replication origin"
     misc_feature    complement(5912..5936)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    5947..5960
                     /label=cloning sites
                     /note="cloning sites"
     misc_feature    complement(6020..6044)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(6447..6463)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(6685..7497)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tcgacggatc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
       61 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      121 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      181 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
      241 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      301 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      361 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      421 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa
      481 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      541 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      601 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      661 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      721 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
      781 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagaagct cgcacattca
      841 gcagcgtttt tcagcgcgtt ttcgatcagc gtttcaatgt tggtatcaac accaggttta
      901 actttgaact tatcggcact gacggttact gattttgaac ttttgctttg ccacggaacg
      961 gtctgcgttg tcgggaagat gcgtgatctg atccttcaac tcagcaaaag ttcgccaata
     1021 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1081 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     1141 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     1201 taacaatttc acacaggaaa cagctatgac catgattacg aattcctagg gcagtaaata
     1261 ggcagtgctc cgcgactaca tgcctcggcc ggcaaaatac cgccgcatgt agagcaggct
     1321 ctccttcacg atcaacgatc ggcatggggc cttcgtgctt gttgagtaat gttatcgctc
     1381 ccatcagagc acgcttggta ctccgggaat cggatggtct gtcgatcatc caaaaaacgc
     1441 tcatgttttc aacctattag gtctgtggtc agctgaccac agaccatcct gctccatact
     1501 cgctaattct agccaaaccg caacgtcccc tgcccgctag ccttcaagag cgccattatc
     1561 atcgggccaa gtgaaaactt cccgagctcg ctccgccgtg tcagatctcg gagatagccc
     1621 ccgggcgaat tgatgaagtt cgctcgctcc aaaatgcacg ccatcgctgc tgccgcattc
     1681 tccggtccca ttgcctcaca cgcgtcttgg taagccgacg ggctgacccc cagcatagac
     1741 cgaaccacca ccgcagccga catgaggtca cgccagctag caaccgcacc gctcggccca
     1801 taattgccaa tggtcgggca tgctttcagg atcatcccga gggggaacgc ttttatcggc
     1861 tcgctccttg cccggtctat ttcactcggc ttagcgccct gctccttttc agagcgaggt
     1921 tcaagttcat taacggattc gggttttgaa ttctgtatgt gctgctcgct ctgggcagca
     1981 ttggtgctat tattttctga attgtctcta atttccaacc ggttgattat ctcttcctgg
     2041 agcatccaca tctcttcgag aattgactct acatcagcaa gcgtcggggc gcgtggaatt
     2101 ctacccacaa gttccacata gacttcctcg acagcttgcc agtcgccctc cgctccctct
     2161 tccatagctg ccgtaattag cttccgaacg tcccgtcggc aaatcgtcag actttctttg
     2221 gccatcctga atgctgctcg atcggccatc acctgctgtg ccatcatcgc tagctcttcg
     2281 gaccgcgcga gaagcggaga caaatcgaag ccaaacgcgc gctcgatctg accagcgcca
     2341 tccttacgag cgtaacgctt tccgttggcg ctatccttcc ggacgatcaa gcctgactcc
     2401 acgagcatgg cgatgtgcct acgcaaagtc gcgccagcca tcccatgcgc ccgaagggca
     2461 agctgagcat tcgacgggaa gacgatcagc tgtgcctcct gacgcaactc cgtttccggg
     2521 tgaaagctca atagcgcatc aaggacggca agactgttgg actggattcc aagtagttcc
     2581 atggccgcgg acgcgtctct aaagaccttc cacttgtccg ctgtcttgcc ttgtttgata
     2641 tcggccagcg ccgtctggcg ccgcacaagc gcaagcgtca ttggccgccg cccgaatggc
     2701 gtcgttacac ttcctgtctg catcatcttt cacctttcag caggcaaagg aaatcagctc
     2761 accaaaacgg cgctaaaaac tcttgacgag gattcgagga aatgcgattc tgttcgcgct
     2821 agagagacag aagggcttcc gcgacggcga cgttgagggg gctcttttct tttgcggttt
     2881 actctccccg tttccgttgg ttctcagcgt ggtacgcttg atacagcgct ggcacatgat
     2941 cgagcacgaa ggtcgcaaaa tcgggcgtcg ccttcctgtc aatcgtgatt tccagtttgg
     3001 ccttgctctg cgtcacctgt gcaattctgg tgccgtctgg ggtggccatg acctcgggaa
     3061 gtccacgcgc aacccgactg ggcttcagac tagcgatcac cgccttgaat cgttctgccg
     3121 atggcagcgc ttgaacttcc tccgacatag catatttagc cacgtcggcc ggtgaagaaa
     3181 ctttctcaat cagctcggca agttgttgcc aactcggccg tccaacacca ggagcggcac
     3241 caatagcatc ggtcagttca gaggggaggg cgtcgacgag cagaagcatc ttggacaaat
     3301 tgctcttgtc gatcgacatc gcggcgatga caatctctcg agaaaactgc ctgttcaggc
     3361 gatgtgcgaa gcgcgccttt tcgatgaagg taagatcttc gcgctcattg ttttcctgac
     3421 cctgtgctac gaccacttgc tcgtccgtca gttcgcgaac gaccgctctg accggaagtc
     3481 cgagttctga aacggcgcgt agccggcggt ggccgaaggc aacctgatat cggcccggct
     3541 ggctcggatg cggtcgcaca aggattggga cttgctgtcc ttgttcccgg atcgaagtaa
     3601 ggagcccgtc aatgtcccct cgcatacgat cctgcacgaa agacggttct attgacgagg
     3661 catccaactc tatcactgcc tgaccttcag cgagacgccg ctcgatctct tcggcacggc
     3721 taagacgatc gttttgctct cgcagtgcgt taccaatgtt cgctgtgagc ttcgttgccg
     3781 gatcgcgctc cttccttgtt acgccgagga gcggcatgga gcggttcttt gccgtcctat
     3841 tgtcggcggg cgacgtctca ggggcgtcag ttgagacgcc aaggatgtgc ttccggctca
     3901 tgtgggccta ccccatgctt ttttgatcag tgtttcgatc tcgtcgttga cggcgttcat
     3961 cgcctccaag gctcgatcat aggtcgagcg cgtgaacagg ccacgctcca cttcgaatag
     4021 agtctggttt gtcaggccag cgtccgaaac cgcggtggtt ttaagcatcg gaaaattgag
     4081 gacattttcg ccaaaaatcg accgcagata acctaccatt tggttctgtg gtccgtcgct
     4141 cggttcgaaa cgggttatca gatagcgcat ccaattaaac ttgaacttgg cgccagcatt
     4201 ctcgatttca cgcaaaaggt tcgatgtcat tgccagaaac tggttcatcg acatcacatc
     4261 cagcatctgc ggatggaccg tgacaagaat ggacgtcgcc gcagtcaatg cggatagcgt
     4321 gagataccca agctggggag ggcagtcgat gaccacgacg tcatagttat ccgcgatatc
     4381 ttcaattact tggctgatgc gaccataaaa gagcgtgtcg ccctctttgc ggttcatcag
     4441 cgcgcgtggc gtatcgtgtt caaactccat cagctcaagg ttaccaggaa tcaggtggag
     4501 gtcgggaatg taagtccctc ggacgactcg ttcgattgcc acctgctcat catcatacct
     4561 tatagcgccg tagagcgttt cgttcgggcc aacgtccgtc tccggttggc tcccaaagag
     4621 tgcagaaagg ctcgcttgag gatcgagatc aatggccaag actcgatatc cgcgcatagc
     4681 gaggtactgc gccagatgcg cggcggtggt ggtcttaccc gacccacctt tgaaattcat
     4741 cacagagata acctgaagct gctcgccgcc tcgacgatgt ggcaggtagc gccggttccc
     4801 gcggccgacc tgatccatat acttccgaat cacatggata tcttcaattg agaacattcg
     4861 cctgccaccc gggctcatgc taacattcaa ctctggcatc tcagacgcgg tctgccgtaa
     4921 atatgactcg ccaacgccga gcagcttgga cgcctccgat ggcccgaatg ttcgaatgcc
     4981 cttctcggaa tgcggcggga aaaccttaag atgatgtgct tgaagttggc tcgagagggc
     5041 atcggcatga cgctccatca aggccgtcaa ccctacaact acaggcgctg cttttaggac
     5101 agacttcgcc atctcaaacc cattccttgc cagtggcgat atttttcgcg aaactggaaa
     5161 agttccgccg ctggcaatta gcgccgattc tgctgtttgg gcaagagctt ttaggttaac
     5221 agaaggttaa cgccctcagg tcgaaaaact ccacccaact gttatttgta tttatttcca
     5281 atgccttaga gagattgcca tttgaatatg ttcatgtatt gttttagtga taatcctaca
     5341 atcgtaaccc aaaaagaggt cgccctctgc gcgccgtcgt ccaatatagg cgaagtcacc
     5401 cttgcgactc aggcggattc taccttgtag gatcgacgat cttgctgcgt tcggatattt
     5461 tcgtggagtt cccgccacag acccggattg aaggcgagat ccagcaactc gcgccagatc
     5521 atcctgtgac ggaactttgg cgcgtgatga ctggccagga cgtcggccga aagagcgaca
     5581 agcagatcac gcttttcgac agcgtcggat ttgcgatcga ggatttttcg gcgctgcgct
     5641 acgtccgcga ccgcgttgag ggatcaagcc acagcagccc actcgacctt ctagccgacc
     5701 cagacgagcc aagggatctt tttggaatgc tgctccgtcg tcaggctttc cgacgtttgg
     5761 gtggttgaac agaagtcatt atcgcacgga atgccaagca ctcccgaagg gaaccctgtg
     5821 gttggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc
     5881 gattattcta ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca
     5941 ctgatagttt aaacggatcc tcctcgaggt acattaaaaa cgtccgcaat gtgttattaa
     6001 gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc
     6061 tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg
     6121 acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat
     6181 tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc
     6241 atgttgattg taacgatgac agagcgttgc tgcctgtgat caaatatcat ctccctcgca
     6301 gagatccgaa ttatcagcct tcttattcat ttctcgctta accgtgacag gctgtcgatc
     6361 ttgagaacta tgccgacata ataggaaatc gctggataaa gccgctgagg aagctgagtg
     6421 gcgctatttc tgcatgcaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa
     6481 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     6541 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     6601 tgcgatttat tcaacaaagc cgccgtcccg tcaagtcagc gtaatgctct gccagtgtta
     6661 caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt
     6721 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga
     6781 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac
     6841 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga
     6901 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt
     6961 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa
     7021 accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg
     7081 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat
     7141 attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc
     7201 agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg
     7261 cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct
     7321 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat
     7381 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc
     7441 catgttggaa tttaatcgcg gcttcgagca agacgtttcc cgttgaatat ggctcataac
     7501 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt
     7561 atcttgtgca atgtaacatc agagattttg agacacaacg tggctttgtt gaataaatcg
     7621 aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt
     7681 ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcatcaaccg
     7741 tggctccctc actttctggc tggatgatgg ggcgattcag gcctggtatg agtcagcaac
     7801 accttcttca cgaggcagac ctc
//
