LOCUS       pRetroX-IRES-DsR        5934 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bicistronic retroviral vector for expressing a gene together with 
            the fluorescent protein DsRed-Express.
ACCESSION   .
VERSION     .
KEYWORDS    pRetroX-IRES-DsRedExpress
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..592
                     /label=5' LTR
                     /note="5' LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    655..1012
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     intron          1075..1278
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of
                     human EF-1-alpha"
     exon            1279..1308
                     /label=EF-1-alpha exon
                     /note="truncated exon 2 from human EF-1-alpha, beginning 
                     with a GT-to-CC mutation that reduces splicing efficiency"
     misc_feature    1362..1389
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    1393..1972
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             1967..2641
                     /label=DsRed-Express
                     /note="rapidly maturing tetrameric variant of DsRed
                     fluorescent protein (Bevis and Glick, 2002)"
     LTR             2697..3290
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     primer_bind     complement(3306..3322)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3330..3346)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3354..3384)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3399..3420)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3708..4296)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4470..5327)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5328..5432)
                     /label=AmpR promoter
     primer_bind     5906..5922
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag
      121 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      241 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      301 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      361 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgggctcaat
      421 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg
      481 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggcct cgctgttcct
      541 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      601 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
      661 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
      721 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
      781 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
      841 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
      901 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
      961 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
     1021 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatacg cgtgtcgagc
     1081 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     1141 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     1201 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     1261 ttttttcttc catttcagcc gtcgtgaaaa ctacccctaa aagccaaaaa aatcgaattc
     1321 ccaaacttaa gcttggtacc gagctcggat ctcacgtggg cccgcggccg cagatctatc
     1381 gatggatccg tgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     1441 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     1501 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     1561 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     1621 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     1681 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     1741 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tcacctcaag
     1801 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     1861 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc
     1921 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg cctcctccga
     1981 ggacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca
     2041 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     2101 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     2161 ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact acaagaagct
     2221 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     2281 gaccgtgacc caggactcct ccctgcagga cggctccttc atctacaagg tgaagttcat
     2341 cggcgtgaac ttcccctccg acggccccgt aatgcagaag aagactatgg gctgggaggc
     2401 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc acaaggccct
     2461 gaagctgaag gacggcggcc actacctggt ggagttcaag tctatctata tggccaagaa
     2521 gcccgtgcag ctgcccggct actactacgt ggactccaag ctggacatca cctcccacaa
     2581 cgaggactac accatcgtgg agcagtacga gcgcgccgag ggccgccacc acctgttcct
     2641 gtagaatgct cgaggataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
     2701 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
     2761 aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga tggaacagct
     2821 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
     2881 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     2941 ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag
     3001 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     3061 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     3121 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta
     3181 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
     3241 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt gaattcgtaa
     3301 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     3361 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3421 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     3481 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     3541 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     3601 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3661 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3721 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3781 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3841 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3901 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3961 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     4021 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     4081 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     4141 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     4201 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     4261 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4321 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4381 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4441 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4501 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4561 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     4621 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     4681 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4741 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     4801 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4861 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4921 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4981 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     5041 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     5101 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     5161 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     5221 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     5281 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     5341 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5401 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     5461 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     5521 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     5581 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     5641 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     5701 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     5761 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     5821 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     5881 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct tatg
//
