LOCUS       40924_36788        7021 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    14..282
                     /label=tetracycline response element
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        315..352
                     /note="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             complement(1017..1637)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     promoter        complement(1649..1978)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             complement(2085..2681)
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     CDS             complement(2699..3115)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    complement(3308..3507)
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     LTR             3572..4041
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     promoter        4072..4176
                     /label=AmpR promoter
     CDS             4177..5034
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5219..5807
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     LTR             complement(6179..6784)
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
ORIGIN
        1 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
       61 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaagt
      121 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
      181 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
      241 aaaagtgaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc
      301 ggtacccggg tcgagtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt
      361 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
      421 ggaccgatcc agcctgcggc cgcttaatta agtttaaacg gatcccccgg gaacaacaac
      481 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
      541 taaaacctct acaaatgtgg tatggctgat tatgatcctg caagcctcgt cgtctggccg
      601 gaccacgcta tctgtgcaag gtccccggac gcgcgctcca tgagcagagc gtcgcgcccc
      661 ctacccaccg tactcgtcaa ttccaagggc atcggtaaac agagcgccgt agggggcgga
      721 gtcgtggggg gtaaatcccg gacccgggga atccccgtcc cccaacatgt ccagatcgaa
      781 atcgtctagc gcgtcggcat gcgccatcgc cacgtcctcg ccgtataagt ggagctcgtc
      841 ccccaggctg acatcggtcg ggggggccgt cgacagtctg cgcgtgtgtc cgcggggaga
      901 aaggacaggc gcggagccgc cagccccgcc tcttcggggg cgtcgtcgtc cgggagatcg
      961 agcaggccct cgatggtaga cccgtaattg tttttcgtac gcgcgcggct gtacgcggac
     1021 ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag gccgaataag
     1081 aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa taatggcggc
     1141 atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc ttgatcttcc
     1201 aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc attctctagt
     1261 gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata ctgtttttct
     1321 gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa agcacatcta
     1381 aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa agggcaaaag
     1441 tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc ccgcttattt
     1501 tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag tttacgggtt
     1561 gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat cactttactt
     1621 ttatctaatc tagacatggt ggaagctttt tgcaaaagcc taggcctcca aaaaagcctc
     1681 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     1741 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     1801 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
     1861 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc
     1921 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat
     1981 tccacaggtc gactagatcg aattctcaat tgttttacgc ggcccgatgc atggggtcgt
     2041 gcgctccttt cggtcgggcg ctgcgggtcg tggggcgggc gtcaggcacc gggcttgcgg
     2101 gtcatgcacc aggtcgcgcg gtccttcggg cactcgacgt cggcggtgac ggtgaagccg
     2161 agccgctcgt agaaggggag gttgcggggc gcggaggtct ccaggaaggc gggcaccccg
     2221 gcgcgctcgg ccgcctccac tccggggagc acgacggcgc tgcccagacc cttgccctgg
     2281 tggtcgggcg agacgccgac ggtggccagg aaccacgcgg gctccttggg ccggtgcggc
     2341 gccaggaggc cttccatctg ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg
     2401 cgcgggccga tctcggcgaa caccgccccc gcttcgacgc tctccggcgt ggtccagacc
     2461 gccaccgcgg cgccgtcgtc cgcgacccac accttgccga tgtcgagccc gacgcgcgtg
     2521 aggaagagtt cttgcagctc ggtgacccgc tcgatgtggc ggtccggatc gacggtgtgg
     2581 cgcgtggcgg ggtagtcggc gaacgcggcg gcgagggtgc gtacggccct ggggacgtcg
     2641 tcgcgggtgg cgaggcgcac cgtgggcttg tactcggtca tggtaagctg atccggccgg
     2701 cgcctagaga aggagtgagg gctggataaa gggaggattg aggcggggtc gaaagaggag
     2761 gttcaagggg gagagacggc gcggatggaa gaagaggagg cggaggctta gggtgtacaa
     2821 agggcttgac ccagggaggg gggtcaaaag ccaaggcttc ccaggtcacg atgtagggga
     2881 cctggtctgg gtgtccatgc gggccaggtg aaaagacctt gatcttaacc tgggtgatga
     2941 ggtctcggtt aaaggtgccg tctcgcggcc atccgacgtt aaaggttggc cattctgcag
     3001 agcagaaggt aacccaacgt ctcttcttga catctaccga ctggttgtga gcgagccgct
     3061 cgacatcttt ccagtgatct aaggtcaaac ttaagggagt ggtaacagtc tggccctaat
     3121 tttcagacaa atacagaaac acagtcagac agagacaaca cagaacgatg ctgcagcaga
     3181 caagacgcgc ggcttcggtt ccaaaccgaa agcaaaaatt cagacggagg cgggaactgt
     3241 tttaggttct cgtctcctac cagaaccaca tatcctgacg gggtcggatt ccacatcgac
     3301 tcccttcctc aggtcgggcc acaaaaacgg cccccaaagt ccctgggacg tctcccaggg
     3361 ttgcggccgg gtgttcagaa ctcgtcagtt ccaccacggg tccgccagat acagagctag
     3421 ttagctaact agtaccgacg caggcgcata aaatcagtca tagacactag acaatcggac
     3481 agacacagat aagttgctgg ccagcttacc tcccggtggt gggtcggtgg tccctgggca
     3541 ggggtctccc gatcccggac gagcccccaa atgaaagacc cccgctgacg ggtagtcaat
     3601 cactcagagg agaccctccc aaggaacagc gagaccacaa gtcggatgca actgcaagag
     3661 ggtttattgg atacacgggt acccgggcga ctcagtcaat cggaggactg gcgccccgag
     3721 tgaggggttg tgggctcttt tattgagctc ggggagcaga agcgcgcgaa cagaagcgag
     3781 aagcgaactg attggttagt tcaaataagg cacagggtca tttcaggtcc ttggggcacc
     3841 ctggaaacat ctgatggttc tctagaaact gctgagggct ggaccgcatc tggggaccat
     3901 ctgttcttgg ccctgagccg gggcaggaac tgcttaccac agatatcctg tttggcccat
     3961 attcagctgt tccatctgtt cttggccctg agccggggca ggaactgctt accacagata
     4021 tcctgtttgg cccatattca ggctgcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4081 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     4141 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     4201 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     4261 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     4321 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     4381 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     4441 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     4501 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     4561 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     4621 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     4681 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     4741 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4801 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4861 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4921 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4981 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5041 tcagaccaag tttactcata tatactttag attgatttgc ggccggccgc aaacttcatt
     5101 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5161 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5221 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5281 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5341 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     5401 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5461 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5521 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5581 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     5641 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5701 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5761 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5821 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5881 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     5941 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgccaatacg
     6001 caaaccgcct ctccccgcgc gttggccgat tcattaatgc aactatggcc atttaatgta
     6061 aatacttaag aaaaaaaacc aaattaattt tgatacatgc tgcatgtgaa gacccccgct
     6121 gacgggtagt caatcactca gaggagaccc tcccaaggca gcgagaccac aagtcggaaa
     6181 tgaaagaccc ccgctgacgg gtagtcaatc actcagagga gaccctccca aggaacagcg
     6241 agaccacaag tcggatgcaa ctgcaagagg gtttattgga tacacgggta cccgggcgac
     6301 tcagtcaatc ggaggactgg cgccccgagt gaggggttgt gggctctttt attgagctcg
     6361 gggagcagaa gcgcgcgaac agaagcgaga agcgaactga ttggttagtt caaataaggc
     6421 acagggtcat ttcaggtcct tggggcaccc tggaaacatc tgatggttct ctagaaactg
     6481 ctgagggctg gaccgcatct ggggaccatc tgttcttggc cctgagccgg ggcaggaact
     6541 gcttaccaca gatatcctgt ttggcccata ttcagctgtt ccatctgttc ttggccctga
     6601 gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag ctgttccatc
     6661 tgttcctgac cttgatctga acttctctat tctcagttat gtatttttcc atgccttgca
     6721 aaatggcgtt acttaagcta gcagatctgc tagcttgcca aacctacagg tggggtcttt
     6781 cattcccccc tttttctgga gactaaataa aatcttttat tttatgcgca catttccccg
     6841 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     6901 gcgtatcacg aggccctttc gtccgcacat ttccccgaaa agtgccacct gacgtctaag
     6961 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     7021 c
//
