LOCUS       62056_18765        5597 bp DNA     circular SYN 12-MAY-2020
DEFINITION  Cloning vector pRcTAL-C1, complete sequence.
ACCESSION   MT233540
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS.
  TITLE     Plants with genetically encoded autoluminescence
  JOURNAL   Nat. Biotechnol. (2020) In press
  PUBMED    32341562
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2020) Laboratory of Chemistry of Metabolic 
            Pathways, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia
REFERENCE   3  (bases 1 to 5597)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol. (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-MAR-2020) Laboratory of Chemistry of Metabolic Pathways, 
            Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     gene            603..2204
                     /gene="tal"
                     /label=tal
     CDS             603..2204
                     /codon_start=1
                     /transl_table=11
                     /gene="tal"
                     /product="TAL"
                     /label=tal
                     /note="derived from R. capsulatus"
                     /protein_id="QJQ48098.1"
                     /translation="MTLQSQTAKDCLALDGALTLVQCEAIATHRSRISVTPALRERCAR
                     AHARLEHAIAEQRHIYGITTGFGPLANRLIGADQGAELQQNLIYHLATGVGPKLSWAEA
                     RALMLARLNSILQGASGASPETIDRIVAVLNAGFAPEVPAQGTVGASGDLTPLAHMVLA
                     LQGRGRMIDPSGRVQEAGAVMDRLCGGPLTLAARDGLALVNGTSAMTAIAALTGVEAAR
                     AIDAALRHSAVLMEVLSGHAEAWHPAFAELRPHPGQLRATERLAQALDGAGRVCRTLTA
                     ARRLTAADLRPEDHPAQDAYSLRVVPQLVGAVWDTLDWHDRVVTCELNSVTDNPIFPEG
                     CAVPALHGGNFMGVHVALASDALNAALVTLAGLVERQIARLTDEKLNKGLPAFLHGGQA
                     GLQSGFMGAQVTATALLAEMRANATPVSVQSLSTNGANQDVVSMGTIAARRARAQLLPL
                     SQIQAILALALAQAMDLLDDPEGQAGWSLTARDLRDRIRAVSPGLRADRPLAGHIEAVA
                     QGLRHPSAAADPPASG"
     misc_feature    2205..2261
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    2385..2506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2513..2968)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2995..3099
                     /label=AmpR promoter
     promoter        3101..3458
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3493..4284
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4519..4566
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4895..5483
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcaccg
      601 ccatgactct tcaatcccaa actgctaagg attgcttggc actggatggt gcactgactt
      661 tggtgcagtg cgaggcaatc gccacgcatc gtagtcgtat ttccgttacg ccagccttgc
      721 gtgaacgttg tgctcgtgcc cacgcacgtt tggagcacgc aatcgcagag cagcgtcata
      781 tttacggtat caccactgga tttggacccc tggctaaccg tcttattgga gccgatcagg
      841 gcgctgagct gcaacagaat ctgatttacc atcttgcaac gggcgttgga cccaagctgt
      901 cctgggcaga ggcccgtgca cttatgttgg cacgtcttaa ttccattttg cagggcgcta
      961 gtggtgcttc tcctgaaacg atcgaccgta tcgtcgcagt acttaacgct ggctttgctc
     1021 ctgaagttcc cgcacaaggt actgttggcg caagtggcga cttgactccc cttgcccaca
     1081 tggtactggc cctgcaaggc cgtggacgta tgatcgatcc tagtggacgt gtgcaagagg
     1141 ctggagccgt catggatcgt ctttgcggag gccctcttac gttggccgca cgtgacggcc
     1201 ttgctctggt caacggtaca agtgctatga ctgctatcgc tgctcttact ggagtcgagg
     1261 ccgctcgtgc tatcgatgct gcacttcgtc actccgccgt tttgatggaa gtcctgtctg
     1321 gacatgcaga agcctggcac cccgcctttg cagagttgag accccaccca ggccagttgc
     1381 gtgctactga acgtttggcc caggccctgg atggcgcagg ccgtgtatgc cgtactctga
     1441 cggctgcccg tcgtcttact gcagccgacc tgcgtccaga ggaccacccc gcacaagacg
     1501 cttattctct gcgtgtcgtt cctcaactgg ttggtgccgt atgggatact cttgactggc
     1561 acgaccgtgt cgtgacctgt gagcttaatt cagtaaccga taaccccatt tttccagagg
     1621 gttgcgccgt tcctgcactt cacggaggaa acttcatggg agtccacgtc gcactggctt
     1681 cagacgcttt gaatgcagcc ctggttaccc ttgccggtct tgtagagcgt caaattgccc
     1741 gtcttaccga cgagaaactt aataaaggtc tgcctgcttt ccttcacggt ggacaggcag
     1801 gccttcagag tggcttcatg ggcgctcagg ttacagcaac tgccctgttg gctgagatgc
     1861 gtgcaaatgc aactccagtc tcagtacaat ccttgtccac aaacggtgca aaccaagacg
     1921 tcgtatctat gggaactatc gccgcacgtc gtgctcgtgc acaattgttg ccactgtcac
     1981 agattcaggc tatcttggct ttggcattgg cccaggctat ggatcttctt gacgatcccg
     2041 agggtcaagc cggatggtct ttgacagccc gtgatttgcg tgaccgtatc cgtgccgttt
     2101 ctcccggact gcgtgccgac cgtccactgg caggccatat cgaagctgtt gctcagggac
     2161 ttcgtcaccc atctgctgca gccgatccac cagcctcagg ttgaagatct cgagctcaag
     2221 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga
     2281 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     2341 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     2401 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     2461 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta
     2521 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     2581 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     2641 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     2701 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     2761 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     2821 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     2881 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     2941 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3001 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3061 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
     3121 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     3181 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     3241 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     3301 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     3361 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     3421 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
     3481 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     3541 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     3601 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     3661 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     3721 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     3781 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     3841 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     3901 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     3961 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     4021 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     4081 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     4141 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     4201 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     4261 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     4321 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     4381 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     4441 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
     4501 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
     4561 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     4621 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     4681 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
     4741 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     4801 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     4861 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4921 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4981 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5041 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5101 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5161 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5221 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5281 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5341 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5401 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5461 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5521 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5581 gtattaccgc catgcat
//
